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Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis

SIMPLE SUMMARY: Senotainia tricuspis is a dipterian endoparasitoid of the honey bee. It is responsible for the severe damage (called senotainiosis) of apiaries in several European, North African and Middle Eastern countries. Despite the availability of data on the infestation percentages and the inc...

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Autores principales: Bedini, Gianluca, Boni, Chiara Benedetta, Coppola, Francesca, Sagona, Simona, Giusti, Matteo, Pinzauti, Mauro, Felicioli, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10231110/
https://www.ncbi.nlm.nih.gov/pubmed/37233043
http://dx.doi.org/10.3390/insects14050415
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author Bedini, Gianluca
Boni, Chiara Benedetta
Coppola, Francesca
Sagona, Simona
Giusti, Matteo
Pinzauti, Mauro
Felicioli, Antonio
author_facet Bedini, Gianluca
Boni, Chiara Benedetta
Coppola, Francesca
Sagona, Simona
Giusti, Matteo
Pinzauti, Mauro
Felicioli, Antonio
author_sort Bedini, Gianluca
collection PubMed
description SIMPLE SUMMARY: Senotainia tricuspis is a dipterian endoparasitoid of the honey bee. It is responsible for the severe damage (called senotainiosis) of apiaries in several European, North African and Middle Eastern countries. Despite the availability of data on the infestation percentages and the increasingly growing awareness of the senotainiosis damage in beekeeping, the aggression and parasitization behavior of S. tricuspis towards A. mellifera remains poorly investigated. In this study, a description of parasitisation behavior, as well as data on the pupation and emergence of S. tricuspis in an apiary in the province of Pisa (Central Italy), is provided. The categories of aggression, beecatcher, chase and parasitization in the attack behavior of S. tricuspis toward western honey bees were identified and described. Moreover, the daily temporal pattern of the number of aggressions showed two main peaks: one during the morning hours and one in the afternoon. Data on the sinking depth of larvae and successful pupation allowed us to hypothesize that mulch and/or minimum soil tillage could prevent severe senotainiosis in apiaries. ABSTRACT: Senotainia tricuspis (Meigen, 1838) is a Sarcophagid dipteran endoparasitoid of Apis mellifera L., and myiasis, caused by this fly, is reported in several European, North African and Middle Eastern countries. Nevertheless, very little knowledge concerning the aggression and parasitisation behavior of S. tricuspis toward A. mellifera is available in the scientific literature, and the temporal pattern of aggression remains unclear. The aim of this investigation was to describe the aggressive behavior of S. tricuspis and to provide data on pupation and adult emergence in order to identify further tools for the control of senotainiosis in beekeeping. Data were collected in an apiary in Pisa province (Tuscany, Italy), where observations of aggressive behavior were conducted indirectly by using a VHS camera and also directly by an observer. Four behavioral categories of the attack were described. A total of 55 aggressions, 21 beecatchers, 104 chases and 6 parasitization events were recorded with the camera. Slow-motion recording analyses of the parasitization episodes resulted in contact of at least 1/6 s between the parasitoid and the host. Through four days of direct observations, a total of 1633 aggression events were recorded. The daily temporal pattern of the number of aggressions showed two main peaks: one during the morning hours (i.e., from 10:00 to 11:00) and one in the afternoon (i.e., from 15:00 to 17:00). The morphometric data on the first-instars of S. tricuspis allowed us to hypothesize a penetration in the bee through its prothoracic spiracle as a modality of entrance in the host body. Third-instars successfully pupate when sinking in topsoil or clay soil, and adults emerge when left to a 4 °C overwintering period of six months. Furthermore, the high mortality rate of those larvae that did not sink and did not pupate successfully suggests that reaching a certain depth in the soil is a determining factor for larvae survival and that mulch and/or minimum soil tillage could prevent severe senotainiosis in apiaries.
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spelling pubmed-102311102023-06-01 Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis Bedini, Gianluca Boni, Chiara Benedetta Coppola, Francesca Sagona, Simona Giusti, Matteo Pinzauti, Mauro Felicioli, Antonio Insects Article SIMPLE SUMMARY: Senotainia tricuspis is a dipterian endoparasitoid of the honey bee. It is responsible for the severe damage (called senotainiosis) of apiaries in several European, North African and Middle Eastern countries. Despite the availability of data on the infestation percentages and the increasingly growing awareness of the senotainiosis damage in beekeeping, the aggression and parasitization behavior of S. tricuspis towards A. mellifera remains poorly investigated. In this study, a description of parasitisation behavior, as well as data on the pupation and emergence of S. tricuspis in an apiary in the province of Pisa (Central Italy), is provided. The categories of aggression, beecatcher, chase and parasitization in the attack behavior of S. tricuspis toward western honey bees were identified and described. Moreover, the daily temporal pattern of the number of aggressions showed two main peaks: one during the morning hours and one in the afternoon. Data on the sinking depth of larvae and successful pupation allowed us to hypothesize that mulch and/or minimum soil tillage could prevent severe senotainiosis in apiaries. ABSTRACT: Senotainia tricuspis (Meigen, 1838) is a Sarcophagid dipteran endoparasitoid of Apis mellifera L., and myiasis, caused by this fly, is reported in several European, North African and Middle Eastern countries. Nevertheless, very little knowledge concerning the aggression and parasitisation behavior of S. tricuspis toward A. mellifera is available in the scientific literature, and the temporal pattern of aggression remains unclear. The aim of this investigation was to describe the aggressive behavior of S. tricuspis and to provide data on pupation and adult emergence in order to identify further tools for the control of senotainiosis in beekeeping. Data were collected in an apiary in Pisa province (Tuscany, Italy), where observations of aggressive behavior were conducted indirectly by using a VHS camera and also directly by an observer. Four behavioral categories of the attack were described. A total of 55 aggressions, 21 beecatchers, 104 chases and 6 parasitization events were recorded with the camera. Slow-motion recording analyses of the parasitization episodes resulted in contact of at least 1/6 s between the parasitoid and the host. Through four days of direct observations, a total of 1633 aggression events were recorded. The daily temporal pattern of the number of aggressions showed two main peaks: one during the morning hours (i.e., from 10:00 to 11:00) and one in the afternoon (i.e., from 15:00 to 17:00). The morphometric data on the first-instars of S. tricuspis allowed us to hypothesize a penetration in the bee through its prothoracic spiracle as a modality of entrance in the host body. Third-instars successfully pupate when sinking in topsoil or clay soil, and adults emerge when left to a 4 °C overwintering period of six months. Furthermore, the high mortality rate of those larvae that did not sink and did not pupate successfully suggests that reaching a certain depth in the soil is a determining factor for larvae survival and that mulch and/or minimum soil tillage could prevent severe senotainiosis in apiaries. MDPI 2023-04-26 /pmc/articles/PMC10231110/ /pubmed/37233043 http://dx.doi.org/10.3390/insects14050415 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bedini, Gianluca
Boni, Chiara Benedetta
Coppola, Francesca
Sagona, Simona
Giusti, Matteo
Pinzauti, Mauro
Felicioli, Antonio
Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis
title Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis
title_full Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis
title_fullStr Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis
title_full_unstemmed Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis
title_short Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis
title_sort host-parasitoid relationship between apis mellifera (linnaeus, 1758) and senotainia tricuspis (meigen, 1838) (diptera, sarcophagidae): fly aggression behavior and infestation rates of senotainiosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10231110/
https://www.ncbi.nlm.nih.gov/pubmed/37233043
http://dx.doi.org/10.3390/insects14050415
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