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Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains

Neural stem cell aging is a fundamental question in neurogenesis. Premature nuclear Pros is considered as an indicator of early neural stem cell aging in Drosophila. The underlying mechanism of how neural stem cells prevent premature nuclear Pros remains largely unknown. Here we identified that two...

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Autores principales: An, Huanping, Yu, Yue, Ren, Xuming, Zeng, Minghua, Bai, Yu, Liu, Tao, Zheng, Huimei, Sang, Rong, Zhang, Fan, Cai, Yu, Xi, Yongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10231327/
https://www.ncbi.nlm.nih.gov/pubmed/37266371
http://dx.doi.org/10.3389/fnmol.2023.1160222
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author An, Huanping
Yu, Yue
Ren, Xuming
Zeng, Minghua
Bai, Yu
Liu, Tao
Zheng, Huimei
Sang, Rong
Zhang, Fan
Cai, Yu
Xi, Yongmei
author_facet An, Huanping
Yu, Yue
Ren, Xuming
Zeng, Minghua
Bai, Yu
Liu, Tao
Zheng, Huimei
Sang, Rong
Zhang, Fan
Cai, Yu
Xi, Yongmei
author_sort An, Huanping
collection PubMed
description Neural stem cell aging is a fundamental question in neurogenesis. Premature nuclear Pros is considered as an indicator of early neural stem cell aging in Drosophila. The underlying mechanism of how neural stem cells prevent premature nuclear Pros remains largely unknown. Here we identified that two pipsqueak family genes, distal antenna (dan) and distal antenna-related (danr), promote the proliferation of neural stem cells (also called neuroblasts, NBs) in third instar larval brains. In the absence of Dan and Danr (dan/danr), the NBs produce fewer daughter cells with smaller lineage sizes. The larval brain NBs in dan/danr clones show premature accumulation of nuclear Prospero (Pros), which usually appears in the terminating NBs at early pupal stage. The premature nuclear Pros leads to NBs cell cycle defects and NB identities loss. Removal of Pros from dan/danr MARCM clones prevents lineage size shrinkage and rescues the loss of NB markers. We propose that the timing of nuclear Pros is after the downregulation of dan/danr in the wt terminating NBs. dan/danr and nuclear Pros are mutually exclusive in NBs. In addition, dan/danr are also required for the late temporal regulator, Grainyhead (Grh), in third instar larval brains. Our study uncovers the novel function of dan/danr in NBs cell fate maintenance. dan/danr antagonize nuclear Pros to prevent NBs aging in Drosophila larval brains.
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spelling pubmed-102313272023-06-01 Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains An, Huanping Yu, Yue Ren, Xuming Zeng, Minghua Bai, Yu Liu, Tao Zheng, Huimei Sang, Rong Zhang, Fan Cai, Yu Xi, Yongmei Front Mol Neurosci Molecular Neuroscience Neural stem cell aging is a fundamental question in neurogenesis. Premature nuclear Pros is considered as an indicator of early neural stem cell aging in Drosophila. The underlying mechanism of how neural stem cells prevent premature nuclear Pros remains largely unknown. Here we identified that two pipsqueak family genes, distal antenna (dan) and distal antenna-related (danr), promote the proliferation of neural stem cells (also called neuroblasts, NBs) in third instar larval brains. In the absence of Dan and Danr (dan/danr), the NBs produce fewer daughter cells with smaller lineage sizes. The larval brain NBs in dan/danr clones show premature accumulation of nuclear Prospero (Pros), which usually appears in the terminating NBs at early pupal stage. The premature nuclear Pros leads to NBs cell cycle defects and NB identities loss. Removal of Pros from dan/danr MARCM clones prevents lineage size shrinkage and rescues the loss of NB markers. We propose that the timing of nuclear Pros is after the downregulation of dan/danr in the wt terminating NBs. dan/danr and nuclear Pros are mutually exclusive in NBs. In addition, dan/danr are also required for the late temporal regulator, Grainyhead (Grh), in third instar larval brains. Our study uncovers the novel function of dan/danr in NBs cell fate maintenance. dan/danr antagonize nuclear Pros to prevent NBs aging in Drosophila larval brains. Frontiers Media S.A. 2023-05-17 /pmc/articles/PMC10231327/ /pubmed/37266371 http://dx.doi.org/10.3389/fnmol.2023.1160222 Text en Copyright © 2023 An, Yu, Ren, Zeng, Bai, Liu, Zheng, Sang, Zhang, Cai and Xi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
An, Huanping
Yu, Yue
Ren, Xuming
Zeng, Minghua
Bai, Yu
Liu, Tao
Zheng, Huimei
Sang, Rong
Zhang, Fan
Cai, Yu
Xi, Yongmei
Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains
title Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains
title_full Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains
title_fullStr Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains
title_full_unstemmed Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains
title_short Pipsqueak family genes dan/danr antagonize nuclear Pros to prevent neural stem cell aging in Drosophila larval brains
title_sort pipsqueak family genes dan/danr antagonize nuclear pros to prevent neural stem cell aging in drosophila larval brains
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10231327/
https://www.ncbi.nlm.nih.gov/pubmed/37266371
http://dx.doi.org/10.3389/fnmol.2023.1160222
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