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A novel home-use culture mechanism for identifying microbial load in urine samples

Diagnosing a urinary tract infection (UTI) is typically a clinical procedure involving multiple steps. The need to perform a test depends on the presence of relevant symptoms. Given the current pandemic situation, visiting a clinic may not be a preferable choice for many users. Many vulnerable group...

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Detalles Bibliográficos
Autores principales: Singh, Monika, Sahai, Riya, Pattnaik, Siddharth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10231770/
https://www.ncbi.nlm.nih.gov/pubmed/37256899
http://dx.doi.org/10.1371/journal.pone.0285881
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author Singh, Monika
Sahai, Riya
Pattnaik, Siddharth
author_facet Singh, Monika
Sahai, Riya
Pattnaik, Siddharth
author_sort Singh, Monika
collection PubMed
description Diagnosing a urinary tract infection (UTI) is typically a clinical procedure involving multiple steps. The need to perform a test depends on the presence of relevant symptoms. Given the current pandemic situation, visiting a clinic may not be a preferable choice for many users. Many vulnerable groups of patients, namely, males with certain predispositions and pregnant women, may not present with symptoms of UTI and may go undiagnosed, which could give rise to a more complicated situation. So far, microbial cultures have been used as the gold standard for the diagnosis of an infection. However, performing microbial cultures currently requires trained professionals and laboratory grade equipment. Therefore, there is a need for a home-use culture kit that can serve this purpose. To our knowledge, no such kit exists. Here, we present a feasibility study of an affordable and easy-to-use home-based setup for quantifying bacterial load in a urine. We believe that such a system can be used by people at home to monitor recurrent UTI infections and also by physicians to remotely monitor and prescribe narrow-spectrum antibiotics for more effective treatment.
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spelling pubmed-102317702023-06-01 A novel home-use culture mechanism for identifying microbial load in urine samples Singh, Monika Sahai, Riya Pattnaik, Siddharth PLoS One Research Article Diagnosing a urinary tract infection (UTI) is typically a clinical procedure involving multiple steps. The need to perform a test depends on the presence of relevant symptoms. Given the current pandemic situation, visiting a clinic may not be a preferable choice for many users. Many vulnerable groups of patients, namely, males with certain predispositions and pregnant women, may not present with symptoms of UTI and may go undiagnosed, which could give rise to a more complicated situation. So far, microbial cultures have been used as the gold standard for the diagnosis of an infection. However, performing microbial cultures currently requires trained professionals and laboratory grade equipment. Therefore, there is a need for a home-use culture kit that can serve this purpose. To our knowledge, no such kit exists. Here, we present a feasibility study of an affordable and easy-to-use home-based setup for quantifying bacterial load in a urine. We believe that such a system can be used by people at home to monitor recurrent UTI infections and also by physicians to remotely monitor and prescribe narrow-spectrum antibiotics for more effective treatment. Public Library of Science 2023-05-31 /pmc/articles/PMC10231770/ /pubmed/37256899 http://dx.doi.org/10.1371/journal.pone.0285881 Text en © 2023 Singh et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Singh, Monika
Sahai, Riya
Pattnaik, Siddharth
A novel home-use culture mechanism for identifying microbial load in urine samples
title A novel home-use culture mechanism for identifying microbial load in urine samples
title_full A novel home-use culture mechanism for identifying microbial load in urine samples
title_fullStr A novel home-use culture mechanism for identifying microbial load in urine samples
title_full_unstemmed A novel home-use culture mechanism for identifying microbial load in urine samples
title_short A novel home-use culture mechanism for identifying microbial load in urine samples
title_sort novel home-use culture mechanism for identifying microbial load in urine samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10231770/
https://www.ncbi.nlm.nih.gov/pubmed/37256899
http://dx.doi.org/10.1371/journal.pone.0285881
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