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Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies
In multiple sclerosis, while remarkable progress has been accomplished to control the inflammatory component of the disease, repair of demyelinated lesions is still an unmet need. Despite encouraging results generated in experimental models, several candidates favouring or promoting remyelination ha...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232271/ https://www.ncbi.nlm.nih.gov/pubmed/36995973 http://dx.doi.org/10.1093/brain/awad051 |
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author | Henriet, Esther Martin, Elodie M Jubin, Pauline Langui, Dominique Mannioui, Abdelkrim Stankoff, Bruno Lubetzki, Catherine Khakhalin, Arseny Zalc, Bernard |
author_facet | Henriet, Esther Martin, Elodie M Jubin, Pauline Langui, Dominique Mannioui, Abdelkrim Stankoff, Bruno Lubetzki, Catherine Khakhalin, Arseny Zalc, Bernard |
author_sort | Henriet, Esther |
collection | PubMed |
description | In multiple sclerosis, while remarkable progress has been accomplished to control the inflammatory component of the disease, repair of demyelinated lesions is still an unmet need. Despite encouraging results generated in experimental models, several candidates favouring or promoting remyelination have not reached the expected outcomes in clinical trials. One possible reason for these failures is that, in most cases, during preclinical testing, efficacy was evaluated on histology only, while functional recovery had not been assessed. We have generated a Xenopus laevis transgenic model Tg(mbp:GFP-NTR) of conditional demyelination in which spontaneous remyelination can be accelerated using candidate molecules. Xenopus laevis is a classic model for in vivo studies of myelination because tadpoles are translucent. We reasoned that demyelination should translate into loss of sensorimotor functions followed by behavioural recovery upon remyelination. To this end, we measured the swimming speed and distance travelled before and after demyelination and during the ongoing spontaneous remyelination and have developed a functional assay based on the visual avoidance of a virtual collision. Here we show that alteration of these functional and clinical performances correlated well with the level of demyelination and that histological remyelination, assayed by counting in vivo the number of myelinating oligodendrocytes in the optic nerve, translated in clinical–functional recovery. This method was further validated in tadpoles treated with pro-remyelinating agents (clemastine, siponimod) showing that increased remyelination in the optic nerve was associated with functional improvement. Our data illustrate the potential interest of correlating histopathological parameters and functional–clinical parameters to screen molecules promoting remyelination in a simple in vivo model of conditional demyelination. |
format | Online Article Text |
id | pubmed-10232271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102322712023-06-01 Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies Henriet, Esther Martin, Elodie M Jubin, Pauline Langui, Dominique Mannioui, Abdelkrim Stankoff, Bruno Lubetzki, Catherine Khakhalin, Arseny Zalc, Bernard Brain Original Article In multiple sclerosis, while remarkable progress has been accomplished to control the inflammatory component of the disease, repair of demyelinated lesions is still an unmet need. Despite encouraging results generated in experimental models, several candidates favouring or promoting remyelination have not reached the expected outcomes in clinical trials. One possible reason for these failures is that, in most cases, during preclinical testing, efficacy was evaluated on histology only, while functional recovery had not been assessed. We have generated a Xenopus laevis transgenic model Tg(mbp:GFP-NTR) of conditional demyelination in which spontaneous remyelination can be accelerated using candidate molecules. Xenopus laevis is a classic model for in vivo studies of myelination because tadpoles are translucent. We reasoned that demyelination should translate into loss of sensorimotor functions followed by behavioural recovery upon remyelination. To this end, we measured the swimming speed and distance travelled before and after demyelination and during the ongoing spontaneous remyelination and have developed a functional assay based on the visual avoidance of a virtual collision. Here we show that alteration of these functional and clinical performances correlated well with the level of demyelination and that histological remyelination, assayed by counting in vivo the number of myelinating oligodendrocytes in the optic nerve, translated in clinical–functional recovery. This method was further validated in tadpoles treated with pro-remyelinating agents (clemastine, siponimod) showing that increased remyelination in the optic nerve was associated with functional improvement. Our data illustrate the potential interest of correlating histopathological parameters and functional–clinical parameters to screen molecules promoting remyelination in a simple in vivo model of conditional demyelination. Oxford University Press 2023-03-30 /pmc/articles/PMC10232271/ /pubmed/36995973 http://dx.doi.org/10.1093/brain/awad051 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Guarantors of Brain. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Original Article Henriet, Esther Martin, Elodie M Jubin, Pauline Langui, Dominique Mannioui, Abdelkrim Stankoff, Bruno Lubetzki, Catherine Khakhalin, Arseny Zalc, Bernard Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies |
title | Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies |
title_full | Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies |
title_fullStr | Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies |
title_full_unstemmed | Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies |
title_short | Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies |
title_sort | monitoring recovery after cns demyelination, a novel tool to de-risk pro-remyelinating strategies |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232271/ https://www.ncbi.nlm.nih.gov/pubmed/36995973 http://dx.doi.org/10.1093/brain/awad051 |
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