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Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient

Helicobacter pylori is involved in the etiology and severity of several gastroduodenal diseases; however, plasticity of the H. pylori genome makes complete genome assembly difficult. We report here the full genomes of H. pylori strains CHC155 and VN1291 isolated from a non-cardia gastric cancer pati...

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Autores principales: Phuc, Bui Hoang, Tuan, Vo Phuoc, Binh, Tran Thanh, Tung, Pham Huu, Tri, Tran Dinh, Dung, Ho Dang Quy, Thuan, Ngo Phuong Minh, Fauzia, Kartika Afrida, Tshibangu-Kabamba, Evariste, Alfaray, Ricky Indra, Saruuljavkhlan, Batsaikhan, Matsumoto, Takashi, Akada, Junko, Yamaoka, Yoshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232435/
https://www.ncbi.nlm.nih.gov/pubmed/37258611
http://dx.doi.org/10.1038/s41598-023-35527-4
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author Phuc, Bui Hoang
Tuan, Vo Phuoc
Binh, Tran Thanh
Tung, Pham Huu
Tri, Tran Dinh
Dung, Ho Dang Quy
Thuan, Ngo Phuong Minh
Fauzia, Kartika Afrida
Tshibangu-Kabamba, Evariste
Alfaray, Ricky Indra
Saruuljavkhlan, Batsaikhan
Matsumoto, Takashi
Akada, Junko
Yamaoka, Yoshio
author_facet Phuc, Bui Hoang
Tuan, Vo Phuoc
Binh, Tran Thanh
Tung, Pham Huu
Tri, Tran Dinh
Dung, Ho Dang Quy
Thuan, Ngo Phuong Minh
Fauzia, Kartika Afrida
Tshibangu-Kabamba, Evariste
Alfaray, Ricky Indra
Saruuljavkhlan, Batsaikhan
Matsumoto, Takashi
Akada, Junko
Yamaoka, Yoshio
author_sort Phuc, Bui Hoang
collection PubMed
description Helicobacter pylori is involved in the etiology and severity of several gastroduodenal diseases; however, plasticity of the H. pylori genome makes complete genome assembly difficult. We report here the full genomes of H. pylori strains CHC155 and VN1291 isolated from a non-cardia gastric cancer patient and a duodenal ulcer patient, respectively, and their virulence demonstrated by in vitro infection. Whole-genome sequences were obtained by combining long- and short-reads with a hybrid-assembly approach. Both CHC155 and VN1291 genome possessed four kinds of genomic island: a cag pathogenicity island (cagPAI), two type 4 secretion system islands within an integrative and conjugative element (tfs ICE), and prophage. CHC155 and VN1291 carried East Asian-type cagA and vacA s1m1, and outer membrane protein genes, including two copies of oipA. Corresponded to genetic determinants of antibiotic resistance, chromosomal mutations were identified in CHC155 (rdxA, gyrA, and 23S rRNA) and VN1291 (rdxA, 23S rRNA, and pbp1A). In vitro infection of AGS cells by both strains induced the cell scattering phenotype, tyrosine phosphorylation of CagA, and promoted high levels of IL8 secretion, indicating fully intact phenotypes of the cagPAI. Virulence genes in CHC155 and VN1291 genomes are crucial for H. pylori pathogenesis and are risk factors in the development of gastric cancer and duodenal ulcer. Our in vitro studies indicate that the strains CHC155 and VN1291 carry the pathogenic potential.
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spelling pubmed-102324352023-06-02 Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient Phuc, Bui Hoang Tuan, Vo Phuoc Binh, Tran Thanh Tung, Pham Huu Tri, Tran Dinh Dung, Ho Dang Quy Thuan, Ngo Phuong Minh Fauzia, Kartika Afrida Tshibangu-Kabamba, Evariste Alfaray, Ricky Indra Saruuljavkhlan, Batsaikhan Matsumoto, Takashi Akada, Junko Yamaoka, Yoshio Sci Rep Article Helicobacter pylori is involved in the etiology and severity of several gastroduodenal diseases; however, plasticity of the H. pylori genome makes complete genome assembly difficult. We report here the full genomes of H. pylori strains CHC155 and VN1291 isolated from a non-cardia gastric cancer patient and a duodenal ulcer patient, respectively, and their virulence demonstrated by in vitro infection. Whole-genome sequences were obtained by combining long- and short-reads with a hybrid-assembly approach. Both CHC155 and VN1291 genome possessed four kinds of genomic island: a cag pathogenicity island (cagPAI), two type 4 secretion system islands within an integrative and conjugative element (tfs ICE), and prophage. CHC155 and VN1291 carried East Asian-type cagA and vacA s1m1, and outer membrane protein genes, including two copies of oipA. Corresponded to genetic determinants of antibiotic resistance, chromosomal mutations were identified in CHC155 (rdxA, gyrA, and 23S rRNA) and VN1291 (rdxA, 23S rRNA, and pbp1A). In vitro infection of AGS cells by both strains induced the cell scattering phenotype, tyrosine phosphorylation of CagA, and promoted high levels of IL8 secretion, indicating fully intact phenotypes of the cagPAI. Virulence genes in CHC155 and VN1291 genomes are crucial for H. pylori pathogenesis and are risk factors in the development of gastric cancer and duodenal ulcer. Our in vitro studies indicate that the strains CHC155 and VN1291 carry the pathogenic potential. Nature Publishing Group UK 2023-05-31 /pmc/articles/PMC10232435/ /pubmed/37258611 http://dx.doi.org/10.1038/s41598-023-35527-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Phuc, Bui Hoang
Tuan, Vo Phuoc
Binh, Tran Thanh
Tung, Pham Huu
Tri, Tran Dinh
Dung, Ho Dang Quy
Thuan, Ngo Phuong Minh
Fauzia, Kartika Afrida
Tshibangu-Kabamba, Evariste
Alfaray, Ricky Indra
Saruuljavkhlan, Batsaikhan
Matsumoto, Takashi
Akada, Junko
Yamaoka, Yoshio
Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient
title Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient
title_full Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient
title_fullStr Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient
title_full_unstemmed Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient
title_short Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient
title_sort comparative genomics of two vietnamese helicobacter pylori strains, chc155 from a non-cardia gastric cancer patient and vn1291 from a duodenal ulcer patient
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232435/
https://www.ncbi.nlm.nih.gov/pubmed/37258611
http://dx.doi.org/10.1038/s41598-023-35527-4
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