NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b

The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the p...

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Detalles Bibliográficos
Autores principales: Hargittay, Bruno, Mineev, Konstantin S., Richter, Christian, Sreeramulu, Sridhar, Jonker, Hendrik R.A., Saxena, Krishna, Schwalbe, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232571/
https://www.ncbi.nlm.nih.gov/pubmed/37118562
http://dx.doi.org/10.1007/s12104-023-10132-8
Descripción
Sumario:The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12104-023-10132-8.

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