NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b

The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the p...

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Autores principales: Hargittay, Bruno, Mineev, Konstantin S., Richter, Christian, Sreeramulu, Sridhar, Jonker, Hendrik R.A., Saxena, Krishna, Schwalbe, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232571/
https://www.ncbi.nlm.nih.gov/pubmed/37118562
http://dx.doi.org/10.1007/s12104-023-10132-8
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author Hargittay, Bruno
Mineev, Konstantin S.
Richter, Christian
Sreeramulu, Sridhar
Jonker, Hendrik R.A.
Saxena, Krishna
Schwalbe, Harald
author_facet Hargittay, Bruno
Mineev, Konstantin S.
Richter, Christian
Sreeramulu, Sridhar
Jonker, Hendrik R.A.
Saxena, Krishna
Schwalbe, Harald
author_sort Hargittay, Bruno
collection PubMed
description The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12104-023-10132-8.
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spelling pubmed-102325712023-06-02 NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b Hargittay, Bruno Mineev, Konstantin S. Richter, Christian Sreeramulu, Sridhar Jonker, Hendrik R.A. Saxena, Krishna Schwalbe, Harald Biomol NMR Assign Article The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12104-023-10132-8. Springer Netherlands 2023-04-29 2023 /pmc/articles/PMC10232571/ /pubmed/37118562 http://dx.doi.org/10.1007/s12104-023-10132-8 Text en © The Author(s) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hargittay, Bruno
Mineev, Konstantin S.
Richter, Christian
Sreeramulu, Sridhar
Jonker, Hendrik R.A.
Saxena, Krishna
Schwalbe, Harald
NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b
title NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b
title_full NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b
title_fullStr NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b
title_full_unstemmed NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b
title_short NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b
title_sort nmr resonance assignment of a fibroblast growth factor 8 splicing isoform b
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232571/
https://www.ncbi.nlm.nih.gov/pubmed/37118562
http://dx.doi.org/10.1007/s12104-023-10132-8
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