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Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes

Tomato, as a typical greenhouse crop, is commonly first planted as seedlings in a variety of substrates before being transplanted into soil. However, there is rare research on the characteristics of the bacterial community in tomato roots under this planting mode. In this study, tomatoes were plante...

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Autores principales: Zhang, Xinjian, Li, Qiang, Zhou, Fangyuan, Fan, Susu, Zhao, Xiaoyan, Zhang, Chi, Yan, Kun, Wu, Xiaoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232838/
https://www.ncbi.nlm.nih.gov/pubmed/37275166
http://dx.doi.org/10.3389/fmicb.2023.1182347
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author Zhang, Xinjian
Li, Qiang
Zhou, Fangyuan
Fan, Susu
Zhao, Xiaoyan
Zhang, Chi
Yan, Kun
Wu, Xiaoqing
author_facet Zhang, Xinjian
Li, Qiang
Zhou, Fangyuan
Fan, Susu
Zhao, Xiaoyan
Zhang, Chi
Yan, Kun
Wu, Xiaoqing
author_sort Zhang, Xinjian
collection PubMed
description Tomato, as a typical greenhouse crop, is commonly first planted as seedlings in a variety of substrates before being transplanted into soil. However, there is rare research on the characteristics of the bacterial community in tomato roots under this planting mode. In this study, tomatoes were planted in pots containing three different cultivation media, including soil and two types of substrates in a greenhouse, followed by a transplanting treatment. After collecting tomato root samples, high-throughput sequencing and bioinformatic analysis were used to compare the differences in bacterial diversity and functions between tomato roots before and after transplanting in different cultivation media. In total, 702776 sequences were obtained, and the OTUs were belonging to 109 genera, 58 families, 41 orders, 14 classes, and 12 phyla. Among the three cultivation media, the β-diversity was significant, and there was a slight difference in bacterial species diversity along with a large difference in their abundance at the genus level. Soil and both substrates had 79 bacterial genera in common, these genera accounted for 68.70%, 76.70%, and 71.17% of the total genera found in the soil, substrate 1, and substrate 2, respectively. After being transplanted from the two substrates to the soil, the bacterial community structure and abundance exhibited similarities with those found in the soil. Furthermore, based on microbial function prediction, the microbial communities in the two-substrate environment demonstrated a greater potential for promoting growth, while the microbial communities in the soil exhibited a greater tendency to exert their antibacterial potential. Our findings offer theoretical support for the creation of artificially reconstructed microbial communities in greenhouse cultivation.
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spelling pubmed-102328382023-06-02 Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes Zhang, Xinjian Li, Qiang Zhou, Fangyuan Fan, Susu Zhao, Xiaoyan Zhang, Chi Yan, Kun Wu, Xiaoqing Front Microbiol Microbiology Tomato, as a typical greenhouse crop, is commonly first planted as seedlings in a variety of substrates before being transplanted into soil. However, there is rare research on the characteristics of the bacterial community in tomato roots under this planting mode. In this study, tomatoes were planted in pots containing three different cultivation media, including soil and two types of substrates in a greenhouse, followed by a transplanting treatment. After collecting tomato root samples, high-throughput sequencing and bioinformatic analysis were used to compare the differences in bacterial diversity and functions between tomato roots before and after transplanting in different cultivation media. In total, 702776 sequences were obtained, and the OTUs were belonging to 109 genera, 58 families, 41 orders, 14 classes, and 12 phyla. Among the three cultivation media, the β-diversity was significant, and there was a slight difference in bacterial species diversity along with a large difference in their abundance at the genus level. Soil and both substrates had 79 bacterial genera in common, these genera accounted for 68.70%, 76.70%, and 71.17% of the total genera found in the soil, substrate 1, and substrate 2, respectively. After being transplanted from the two substrates to the soil, the bacterial community structure and abundance exhibited similarities with those found in the soil. Furthermore, based on microbial function prediction, the microbial communities in the two-substrate environment demonstrated a greater potential for promoting growth, while the microbial communities in the soil exhibited a greater tendency to exert their antibacterial potential. Our findings offer theoretical support for the creation of artificially reconstructed microbial communities in greenhouse cultivation. Frontiers Media S.A. 2023-05-18 /pmc/articles/PMC10232838/ /pubmed/37275166 http://dx.doi.org/10.3389/fmicb.2023.1182347 Text en Copyright © 2023 Zhang, Li, Zhou, Fan, Zhao, Zhang, Yan and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Xinjian
Li, Qiang
Zhou, Fangyuan
Fan, Susu
Zhao, Xiaoyan
Zhang, Chi
Yan, Kun
Wu, Xiaoqing
Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
title Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
title_full Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
title_fullStr Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
title_full_unstemmed Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
title_short Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
title_sort effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232838/
https://www.ncbi.nlm.nih.gov/pubmed/37275166
http://dx.doi.org/10.3389/fmicb.2023.1182347
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