Cargando…

Development of a universal real-time RT-PCR assay for detection of pan-SARS-coronaviruses with an RNA-based internal control

The current pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for rapid diagnostic assays to prompt intensified virological monitoring both in human and wild animal populations. To date, there are no clinical validated assays for pan-SARS-c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yu, Beibei, Xu, Changping, Huang, Shiwang, Ni, Jun, Zhou, Jiancang, Zhang, Yuting, Wu, Maomao, Zhang, Jun, Fang, Lei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232947/ https://www.ncbi.nlm.nih.gov/pubmed/37275136 http://dx.doi.org/10.3389/fmicb.2023.1181097

Ejemplares similares

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

The Development and Application of the Two Real-Time RT-PCR Assays to Detect the Pathogen of HFMD
por: Cui, Aili, et al.
Publicado: (2013)

A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
por: Wang, Xinye, et al.
Publicado: (2021)

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses
por: Lazov, Christina M., et al.
Publicado: (2023)

Real-Time RT-PCR Detection of Equine Coronavirus
por: Miszczak, Fabien, et al.
Publicado: (2015)

Detection of Apparent Early Rabies Infection by LN34 Pan-Lyssavirus Real-Time RT-PCR Assay in Pennsylvania
por: Dettinger, Lisa, et al.
Publicado: (2022)

An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes
por: Sun, Junfeng, et al.
Publicado: (2015)

Development of a universal RT-PCR assay for grapevine vitiviruses
por: Diaz-Lara, Alfredo, et al.
Publicado: (2020)

Comparative Evaluation of Standard RT-PCR Assays and Commercial Real-Time RT-PCR Kits for Detection of Lassa Virus
por: Luo, Xue-Lian, et al.
Publicado: (2023)

Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay
por: Decaro, Nicola, et al.
Publicado: (2008)

A Real-Time RT-PCR Assay for Genotyping of Rotavirus
por: Mousavi-Nasab, Seyed Dawood, et al.
Publicado: (2020)

A Pan-Lyssavirus Taqman Real-Time RT-PCR Assay for the Detection of Highly Variable Rabies virus and Other Lyssaviruses
por: Wadhwa, Ashutosh, et al.
Publicado: (2017)

Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics
por: Gigante, Crystal M., et al.
Publicado: (2018)

Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera
por: Holbrook, Myndi G., et al.
Publicado: (2021)

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
por: Vijgen, Leen, et al.
Publicado: (2007)

Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis
por: Soma, Takehisa
Publicado: (2015)

A RT-PCR assay for the detection of coronaviruses from four genera
por: Xiu, Leshan, et al.
Publicado: (2020)

A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray
por: Vina-Rodriguez, Ariel, et al.
Publicado: (2017)

Early diagnosis of SARS Coronavirus infection by real time RT-PCR
por: Poon, Leo L.M., et al.
Publicado: (2003)

Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays
por: Lu, Roujian, et al.
Publicado: (2012)

Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus
por: Amer, Haitham M., et al.
Publicado: (2011)

Universal Single-Probe RT-PCR Assay for Diagnosis of Dengue Virus Infections
por: Alm, Erik, et al.
Publicado: (2014)

Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
por: Piqueur, Marian AC, et al.
Publicado: (2009)

Detection of influenza C virus by a real‐time RT‐PCR assay
por: Pabbaraju, Kanti, et al.
Publicado: (2013)

Detection of enteroviruses and parechoviruses by a multiplex real-time RT-PCR assay
por: Pabbaraju, Kanti, et al.
Publicado: (2015)

A One-Step, Triplex, Real-Time RT-PCR Assay for the Simultaneous Detection of Enterovirus 71, Coxsackie A16 and Pan-Enterovirus in a Single Tube
por: Zhang, Shiyin, et al.
Publicado: (2014)

Use of Chest CT in Combination with Negative RT-PCR Assay for the 2019 Novel Coronavirus but High Clinical Suspicion
por: Huang, Peikai, et al.
Publicado: (2020)

Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay
por: Ma, Lei, et al.
Publicado: (2018)

A quantitative real-time RT-PCR assay for mature C. albicans biofilms
por: Xie, Zhihong, et al.
Publicado: (2011)

Real Time RT-PCR Assays for Detection and Typing of African Horse Sickness Virus
por: Bachanek-Bankowska, Katarzyna, et al.
Publicado: (2014)

Development and Evaluation of Real Time RT-PCR Assays for Detection and Typing of Bluetongue Virus
por: Maan, Sushila, et al.
Publicado: (2016)

Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus
por: Niu, Peihua, et al.
Publicado: (2020)

Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
por: Adachi, D., et al.
Publicado: (2004)

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
por: Poon, Leo L.M, et al.
Publicado: (2004)

Development of a real-time TaqMan(®) RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation
por: Meir, Rosie, et al.
Publicado: (2010)

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
por: Waggoner, Jesse J., et al.
Publicado: (2020)

A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses
por: Wan, Zhenzhou, et al.
Publicado: (2016)

Clinical features of atypical 2019 novel coronavirus pneumonia with an initially negative RT-PCR assay
por: Hao, Wendong, et al.
Publicado: (2020)

Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
por: Hosmillo, Myra D.T., et al.
Publicado: (2010)

Development, Evaluation, and Clinical Application of PRRSV-2 Vaccine-like Real-Time RT-PCR Assays
por: Rawal, Gaurav, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_vi93oq7s0bf9vdf6uv22gf0mug