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Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury

The mortality rate associated with acute lung injury (ALI) and its severe form, acute respiratory distress syndrome, is high. Induced pluripotent stem cell (iPSC) therapy is a potential treatment method for ALI, but its therapeutic efficacy is limited in injured lungs. Nitric oxide (NO) has various...

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Autores principales: Cui, Anfeng, Li, Shirui, Li, Yijun, Yang, Dawei, Huang, Jiongwei, Wang, Xuemeng, Song, Nana, Chen, Fuchen, Chen, Sifeng, Xiang, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232993/
https://www.ncbi.nlm.nih.gov/pubmed/37275899
http://dx.doi.org/10.3389/fimmu.2023.1136290
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author Cui, Anfeng
Li, Shirui
Li, Yijun
Yang, Dawei
Huang, Jiongwei
Wang, Xuemeng
Song, Nana
Chen, Fuchen
Chen, Sifeng
Xiang, Meng
author_facet Cui, Anfeng
Li, Shirui
Li, Yijun
Yang, Dawei
Huang, Jiongwei
Wang, Xuemeng
Song, Nana
Chen, Fuchen
Chen, Sifeng
Xiang, Meng
author_sort Cui, Anfeng
collection PubMed
description The mortality rate associated with acute lung injury (ALI) and its severe form, acute respiratory distress syndrome, is high. Induced pluripotent stem cell (iPSC) therapy is a potential treatment method for ALI, but its therapeutic efficacy is limited in injured lungs. Nitric oxide (NO) has various physiological actions. The current study investigated the effect of iPSCs pretreated with NO donors in paraquat (PQ)-induced ALI mouse model. Male C57BL/6 mice were intraperitoneally injected with PQ, followed by infusion of phosphate-buffered saline, iPSCs, L-arginine pretreated iPSCs, or Nitro-L-arginine methylester (L-NAME) pretreated iPSCs through the tail veins. Histopathological changes, pulmonary microvascular permeability, and inflammatory cytokine levels were analyzed after 3 or 28 d. The effects on iPSC proliferation, migration, and adhesion were evaluated in vitro. More L-arginine-pretreated iPSCs were selectively trafficked into the injured pulmonary tissue of mice with LPS-induced ALI, drastically diminishing the histopathologic changes and inflammatory cytokine levels (IL-1β and IL-6). There was also markedly improved pulmonary microvascular permeability and pulmonary function. The NO inhibitor abolished the protective effects of iPSCs. In addition, the ability of L-arginine to promote the proliferation and migration of iPSCs was decreased by L-NAME pretreatment, suggesting that NO might mediate the therapeutic benefits of iPSC. The improvement of the iPSC physiological changes by the endogenous gaseous molecule NO reduces lung injury severity. L-Arginine represents a pharmacologically important strategy for enhancing the therapeutic potential of iPSCs.
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spelling pubmed-102329932023-06-02 Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury Cui, Anfeng Li, Shirui Li, Yijun Yang, Dawei Huang, Jiongwei Wang, Xuemeng Song, Nana Chen, Fuchen Chen, Sifeng Xiang, Meng Front Immunol Immunology The mortality rate associated with acute lung injury (ALI) and its severe form, acute respiratory distress syndrome, is high. Induced pluripotent stem cell (iPSC) therapy is a potential treatment method for ALI, but its therapeutic efficacy is limited in injured lungs. Nitric oxide (NO) has various physiological actions. The current study investigated the effect of iPSCs pretreated with NO donors in paraquat (PQ)-induced ALI mouse model. Male C57BL/6 mice were intraperitoneally injected with PQ, followed by infusion of phosphate-buffered saline, iPSCs, L-arginine pretreated iPSCs, or Nitro-L-arginine methylester (L-NAME) pretreated iPSCs through the tail veins. Histopathological changes, pulmonary microvascular permeability, and inflammatory cytokine levels were analyzed after 3 or 28 d. The effects on iPSC proliferation, migration, and adhesion were evaluated in vitro. More L-arginine-pretreated iPSCs were selectively trafficked into the injured pulmonary tissue of mice with LPS-induced ALI, drastically diminishing the histopathologic changes and inflammatory cytokine levels (IL-1β and IL-6). There was also markedly improved pulmonary microvascular permeability and pulmonary function. The NO inhibitor abolished the protective effects of iPSCs. In addition, the ability of L-arginine to promote the proliferation and migration of iPSCs was decreased by L-NAME pretreatment, suggesting that NO might mediate the therapeutic benefits of iPSC. The improvement of the iPSC physiological changes by the endogenous gaseous molecule NO reduces lung injury severity. L-Arginine represents a pharmacologically important strategy for enhancing the therapeutic potential of iPSCs. Frontiers Media S.A. 2023-05-18 /pmc/articles/PMC10232993/ /pubmed/37275899 http://dx.doi.org/10.3389/fimmu.2023.1136290 Text en Copyright © 2023 Cui, Li, Li, Yang, Huang, Wang, Song, Chen, Chen and Xiang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Cui, Anfeng
Li, Shirui
Li, Yijun
Yang, Dawei
Huang, Jiongwei
Wang, Xuemeng
Song, Nana
Chen, Fuchen
Chen, Sifeng
Xiang, Meng
Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
title Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
title_full Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
title_fullStr Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
title_full_unstemmed Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
title_short Nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
title_sort nitric oxide-mediated the therapeutic properties of induced pluripotent stem cell for paraquat-induced acute lung injury
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232993/
https://www.ncbi.nlm.nih.gov/pubmed/37275899
http://dx.doi.org/10.3389/fimmu.2023.1136290
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