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Recent progress on single-molecule localization microscopy

Super-resolution imaging based on single-molecule localization has been developed for more than a decade. These techniques can break through diffraction limit of fluorescent microscopy and initially improve the resolution by an order of magnitude to ~20 nm, by introducing photoactivatable/photoswitc...

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Detalles Bibliográficos
Autores principales: Gu, Lusheng, Ji, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biophysics Reports Editorial Office 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233386/
https://www.ncbi.nlm.nih.gov/pubmed/37288101
http://dx.doi.org/10.52601/bpr.2021.210023
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author Gu, Lusheng
Ji, Wei
author_facet Gu, Lusheng
Ji, Wei
author_sort Gu, Lusheng
collection PubMed
description Super-resolution imaging based on single-molecule localization has been developed for more than a decade. These techniques can break through diffraction limit of fluorescent microscopy and initially improve the resolution by an order of magnitude to ~20 nm, by introducing photoactivatable/photoswitching probes and centroid fitting method. As the demand of biological research, the localization precision of single-molecules was further improved by several state-of-the-art methods in the past several years. This review focuses on the latest developed techniques which have greatly improved the performance of single-molecule localization microscopy, from measurement principle to hardware design. These methods are essential for the study of nanostructures and biomacromolecule dynamics inside of cells.
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spelling pubmed-102333862023-06-07 Recent progress on single-molecule localization microscopy Gu, Lusheng Ji, Wei Biophys Rep Mini-Review Super-resolution imaging based on single-molecule localization has been developed for more than a decade. These techniques can break through diffraction limit of fluorescent microscopy and initially improve the resolution by an order of magnitude to ~20 nm, by introducing photoactivatable/photoswitching probes and centroid fitting method. As the demand of biological research, the localization precision of single-molecules was further improved by several state-of-the-art methods in the past several years. This review focuses on the latest developed techniques which have greatly improved the performance of single-molecule localization microscopy, from measurement principle to hardware design. These methods are essential for the study of nanostructures and biomacromolecule dynamics inside of cells. Biophysics Reports Editorial Office 2021-10-31 /pmc/articles/PMC10233386/ /pubmed/37288101 http://dx.doi.org/10.52601/bpr.2021.210023 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mini-Review
Gu, Lusheng
Ji, Wei
Recent progress on single-molecule localization microscopy
title Recent progress on single-molecule localization microscopy
title_full Recent progress on single-molecule localization microscopy
title_fullStr Recent progress on single-molecule localization microscopy
title_full_unstemmed Recent progress on single-molecule localization microscopy
title_short Recent progress on single-molecule localization microscopy
title_sort recent progress on single-molecule localization microscopy
topic Mini-Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233386/
https://www.ncbi.nlm.nih.gov/pubmed/37288101
http://dx.doi.org/10.52601/bpr.2021.210023
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