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Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo

[Image: see text] MicroRNAs (miRNAs) play an essential role in cancer therapy, but the disadvantages of its poor inherent stability, rapid clearance, and low delivery efficiency affect the therapeutic efficiency. Loading miRNAs by nanoformulations can improve their bioavailability and enhance therap...

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Autores principales: Zhang, Shiwen, Pang, Siyan, Pei, Wenhao, Zhu, Haitao, Shi, Yingxiang, Liu, Ziyang, Mao, Lingyu, Shi, Xiuru, Tao, Shuang, Geng, Chenchen, Chen, Sulian, Yang, Linnan, Chen, Changjie, Yang, Qingling, Wang, Wenrui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233669/
https://www.ncbi.nlm.nih.gov/pubmed/37273596
http://dx.doi.org/10.1021/acsomega.2c07866
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author Zhang, Shiwen
Pang, Siyan
Pei, Wenhao
Zhu, Haitao
Shi, Yingxiang
Liu, Ziyang
Mao, Lingyu
Shi, Xiuru
Tao, Shuang
Geng, Chenchen
Chen, Sulian
Yang, Linnan
Chen, Changjie
Yang, Qingling
Wang, Wenrui
author_facet Zhang, Shiwen
Pang, Siyan
Pei, Wenhao
Zhu, Haitao
Shi, Yingxiang
Liu, Ziyang
Mao, Lingyu
Shi, Xiuru
Tao, Shuang
Geng, Chenchen
Chen, Sulian
Yang, Linnan
Chen, Changjie
Yang, Qingling
Wang, Wenrui
author_sort Zhang, Shiwen
collection PubMed
description [Image: see text] MicroRNAs (miRNAs) play an essential role in cancer therapy, but the disadvantages of its poor inherent stability, rapid clearance, and low delivery efficiency affect the therapeutic efficiency. Loading miRNAs by nanoformulations can improve their bioavailability and enhance therapeutic efficiency, which is an effective miRNA delivery strategy. In this study, we synthesized layered double hydroxides (LDH), which are widely used as carriers of drugs or genes due to the characteristics of good biocompatibility, high loading capacity, and pH sensitivity. We loaded the suppressor oncogene miR-30a on LDH nanomaterials (LDH@miR-30a) and determined the mass ratio of miRNA binding to LDH by agarose gel electrophoresis. LDH@miR-30a was able to escape the lysosomal pathway and was successfully phagocytosed by breast cancer SKBR3 cells and remained detectable in the cells after 24 h of co-incubation. In vitro experiments showed that LDH@miR-30a-treated SKBR3 cells showed decreased proliferation and cell cycle arrest in the G0/G1 phase and LDH@miR-30a was able to regulate the epithelial–mesenchymal transition (EMT) process and inhibit cell migration and invasion by targeting SNAI1. Meanwhile, in vivo experiments showed that nude mice treated with LDH@miR-30a showed a significant reduction in their solid tumors and no significant impairment of vital organs was observed. In conclusion, LDH@miR-30a is an effective drug delivery system for the treatment of breast cancer.
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spelling pubmed-102336692023-06-02 Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo Zhang, Shiwen Pang, Siyan Pei, Wenhao Zhu, Haitao Shi, Yingxiang Liu, Ziyang Mao, Lingyu Shi, Xiuru Tao, Shuang Geng, Chenchen Chen, Sulian Yang, Linnan Chen, Changjie Yang, Qingling Wang, Wenrui ACS Omega [Image: see text] MicroRNAs (miRNAs) play an essential role in cancer therapy, but the disadvantages of its poor inherent stability, rapid clearance, and low delivery efficiency affect the therapeutic efficiency. Loading miRNAs by nanoformulations can improve their bioavailability and enhance therapeutic efficiency, which is an effective miRNA delivery strategy. In this study, we synthesized layered double hydroxides (LDH), which are widely used as carriers of drugs or genes due to the characteristics of good biocompatibility, high loading capacity, and pH sensitivity. We loaded the suppressor oncogene miR-30a on LDH nanomaterials (LDH@miR-30a) and determined the mass ratio of miRNA binding to LDH by agarose gel electrophoresis. LDH@miR-30a was able to escape the lysosomal pathway and was successfully phagocytosed by breast cancer SKBR3 cells and remained detectable in the cells after 24 h of co-incubation. In vitro experiments showed that LDH@miR-30a-treated SKBR3 cells showed decreased proliferation and cell cycle arrest in the G0/G1 phase and LDH@miR-30a was able to regulate the epithelial–mesenchymal transition (EMT) process and inhibit cell migration and invasion by targeting SNAI1. Meanwhile, in vivo experiments showed that nude mice treated with LDH@miR-30a showed a significant reduction in their solid tumors and no significant impairment of vital organs was observed. In conclusion, LDH@miR-30a is an effective drug delivery system for the treatment of breast cancer. American Chemical Society 2023-05-15 /pmc/articles/PMC10233669/ /pubmed/37273596 http://dx.doi.org/10.1021/acsomega.2c07866 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Zhang, Shiwen
Pang, Siyan
Pei, Wenhao
Zhu, Haitao
Shi, Yingxiang
Liu, Ziyang
Mao, Lingyu
Shi, Xiuru
Tao, Shuang
Geng, Chenchen
Chen, Sulian
Yang, Linnan
Chen, Changjie
Yang, Qingling
Wang, Wenrui
Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo
title Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo
title_full Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo
title_fullStr Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo
title_full_unstemmed Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo
title_short Layered Double Hydroxide-Loaded miR-30a for the Treatment of Breast Cancer In Vitro and In Vivo
title_sort layered double hydroxide-loaded mir-30a for the treatment of breast cancer in vitro and in vivo
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233669/
https://www.ncbi.nlm.nih.gov/pubmed/37273596
http://dx.doi.org/10.1021/acsomega.2c07866
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