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Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)

This study aimed to investigate Pinus koraiensis cone essential oil (PEO) as a methane (CH(4)) inhibitor and determine its impact on the taxonomic and functional characteristics of the rumen microbiota in goats. A total of 10 growing Korean native goats (Capra hircus coreanae, 29.9 ± 1.58 kg, male)...

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Autores principales: Choi, Youyoung, Lee, Shin Ja, Kim, Hyun Sang, Eom, Jun Sik, Jo, Seong Uk, Guan, Le Luo, Seo, Jakyeom, Park, Tansol, Lee, Yookyung, Lee, Sang Suk, Lee, Sung Sill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234127/
https://www.ncbi.nlm.nih.gov/pubmed/37275608
http://dx.doi.org/10.3389/fvets.2023.1168237
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author Choi, Youyoung
Lee, Shin Ja
Kim, Hyun Sang
Eom, Jun Sik
Jo, Seong Uk
Guan, Le Luo
Seo, Jakyeom
Park, Tansol
Lee, Yookyung
Lee, Sang Suk
Lee, Sung Sill
author_facet Choi, Youyoung
Lee, Shin Ja
Kim, Hyun Sang
Eom, Jun Sik
Jo, Seong Uk
Guan, Le Luo
Seo, Jakyeom
Park, Tansol
Lee, Yookyung
Lee, Sang Suk
Lee, Sung Sill
author_sort Choi, Youyoung
collection PubMed
description This study aimed to investigate Pinus koraiensis cone essential oil (PEO) as a methane (CH(4)) inhibitor and determine its impact on the taxonomic and functional characteristics of the rumen microbiota in goats. A total of 10 growing Korean native goats (Capra hircus coreanae, 29.9 ± 1.58 kg, male) were assigned to different dietary treatments: control (CON; basal diet without additive) and PEO (basal diet +1 g/d of PEO) by a 2 × 2 crossover design. Methane measurements were conducted every 4 consecutive days for 17–20 days using a laser CH(4) detector. Samples of rumen fluid and feces were collected during each experimental period to evaluate the biological effects and dry matter (DM) digestibility after PEO oral administration. The rumen microbiota was analyzed via 16S rRNA gene amplicon sequencing. The PEO oral administration resulted in reduced CH(4) emission (eructation CH(4)/body weight(0.75), p = 0.079) without affecting DM intake; however, it lowered the total volatile fatty acids (p = 0.041), molar proportion of propionate (p = 0.075), and ammonia nitrogen (p = 0.087) in the rumen. Blood metabolites (i.e., albumin, alanine transaminase/serum glutamic pyruvate transaminase, creatinine, and triglyceride) were significantly affected (p < 0.05) by PEO oral administration. The absolute fungal abundance (p = 0.009) was reduced by PEO oral administration, whereas ciliate protozoa, total bacteria, and methanogen abundance were not affected. The composition of rumen prokaryotic microbiota was altered by PEO oral administration with lower evenness (p = 0.054) observed for the PEO group than the CON group. Moreover, PICRUSt2 analysis revealed that the metabolic pathways of prokaryotic bacteria, such as pyruvate metabolism, were enriched in the PEO group. We also identified the Rikenellaceae RC9 gut group as the taxa potentially contributing to the enriched KEGG modules for histidine biosynthesis and pyruvate oxidation in the rumen of the PEO group using the FishTaco analysis. The entire co-occurrence networks showed that more nodes and edges were detected in the PEO group. Overall, these findings provide an understanding of how PEO oral administration affects CH(4) emission and rumen prokaryotic microbiota composition and function. This study may help develop potential manipulation strategies to find new essential oils to mitigate enteric CH(4) emissions from ruminants.
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spelling pubmed-102341272023-06-02 Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae) Choi, Youyoung Lee, Shin Ja Kim, Hyun Sang Eom, Jun Sik Jo, Seong Uk Guan, Le Luo Seo, Jakyeom Park, Tansol Lee, Yookyung Lee, Sang Suk Lee, Sung Sill Front Vet Sci Veterinary Science This study aimed to investigate Pinus koraiensis cone essential oil (PEO) as a methane (CH(4)) inhibitor and determine its impact on the taxonomic and functional characteristics of the rumen microbiota in goats. A total of 10 growing Korean native goats (Capra hircus coreanae, 29.9 ± 1.58 kg, male) were assigned to different dietary treatments: control (CON; basal diet without additive) and PEO (basal diet +1 g/d of PEO) by a 2 × 2 crossover design. Methane measurements were conducted every 4 consecutive days for 17–20 days using a laser CH(4) detector. Samples of rumen fluid and feces were collected during each experimental period to evaluate the biological effects and dry matter (DM) digestibility after PEO oral administration. The rumen microbiota was analyzed via 16S rRNA gene amplicon sequencing. The PEO oral administration resulted in reduced CH(4) emission (eructation CH(4)/body weight(0.75), p = 0.079) without affecting DM intake; however, it lowered the total volatile fatty acids (p = 0.041), molar proportion of propionate (p = 0.075), and ammonia nitrogen (p = 0.087) in the rumen. Blood metabolites (i.e., albumin, alanine transaminase/serum glutamic pyruvate transaminase, creatinine, and triglyceride) were significantly affected (p < 0.05) by PEO oral administration. The absolute fungal abundance (p = 0.009) was reduced by PEO oral administration, whereas ciliate protozoa, total bacteria, and methanogen abundance were not affected. The composition of rumen prokaryotic microbiota was altered by PEO oral administration with lower evenness (p = 0.054) observed for the PEO group than the CON group. Moreover, PICRUSt2 analysis revealed that the metabolic pathways of prokaryotic bacteria, such as pyruvate metabolism, were enriched in the PEO group. We also identified the Rikenellaceae RC9 gut group as the taxa potentially contributing to the enriched KEGG modules for histidine biosynthesis and pyruvate oxidation in the rumen of the PEO group using the FishTaco analysis. The entire co-occurrence networks showed that more nodes and edges were detected in the PEO group. Overall, these findings provide an understanding of how PEO oral administration affects CH(4) emission and rumen prokaryotic microbiota composition and function. This study may help develop potential manipulation strategies to find new essential oils to mitigate enteric CH(4) emissions from ruminants. Frontiers Media S.A. 2023-05-18 /pmc/articles/PMC10234127/ /pubmed/37275608 http://dx.doi.org/10.3389/fvets.2023.1168237 Text en Copyright © 2023 Choi, Lee, Kim, Eom, Jo, Guan, Seo, Park, Lee, Lee and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Choi, Youyoung
Lee, Shin Ja
Kim, Hyun Sang
Eom, Jun Sik
Jo, Seong Uk
Guan, Le Luo
Seo, Jakyeom
Park, Tansol
Lee, Yookyung
Lee, Sang Suk
Lee, Sung Sill
Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)
title Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)
title_full Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)
title_fullStr Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)
title_full_unstemmed Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)
title_short Oral administration of Pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in Korean native goat (Capra hircus coreanae)
title_sort oral administration of pinus koraiensis cone essential oil reduces rumen methane emission by altering the rumen microbial composition and functions in korean native goat (capra hircus coreanae)
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234127/
https://www.ncbi.nlm.nih.gov/pubmed/37275608
http://dx.doi.org/10.3389/fvets.2023.1168237
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