Cargando…
Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment effic...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234675/ https://www.ncbi.nlm.nih.gov/pubmed/37274628 http://dx.doi.org/10.1063/5.0152846 |
_version_ | 1785052550392709120 |
---|---|
author | Pan, Ting Zhang, Dinghu Wu, Xiaoxia Li, Zihou Zeng, Hui Xu, Xiawei Zhang, Chenguang He, Yiwei Gong, Yuanchuan Wang, Pin Mao, Quanliang Yao, Junlie Lin, Jie Wu, Aiguo Shao, Guoliang |
author_facet | Pan, Ting Zhang, Dinghu Wu, Xiaoxia Li, Zihou Zeng, Hui Xu, Xiawei Zhang, Chenguang He, Yiwei Gong, Yuanchuan Wang, Pin Mao, Quanliang Yao, Junlie Lin, Jie Wu, Aiguo Shao, Guoliang |
author_sort | Pan, Ting |
collection | PubMed |
description | The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment efficiency. Surface-enhanced Raman spectroscopy (SERS) and magnetic resonance (MR) imaging exhibit great potential for early TNBC diagnosis. SERS, an optical imaging mode, has the advantages of high detection sensitivity, good spatial resolution, and “fingerprint” spectral characteristics; however, the shallow detection penetration of SERS bioprobes limits its application in vivo. MR has the advantages of allowing deep penetration with no radiation; however, its spatial resolution needs to be improved. SERS and MR have complementary imaging features for tumor marker detection. In this study, gold nanorod and ultrasmall iron oxide nanoparticle composites were developed as dual-modal bioprobes for SERS-MRI to detect PD-L1 expression. Anti-PD-L1 (aPD-L1) was utilized to improve the targeting ability and specificity of PD-L1 expression detection. TNBC cells expressing PD-L1 were accurately detected via the SERS imaging mode in vitro, which can image at the single-cell level. In addition, bioprobe accumulation in PD-L1 expression-related tumor-bearing mice was simply and dynamically monitored and analyzed in vivo using MR and SERS. To the best of our knowledge, this is the first time a SERS-MRI dual-modal bioprobe combined with a PD-L1 antibody has been successfully used to detect PD-L1 expression in TNBC. This work paves the way for the design of high-performance bioprobe-based contrast agents for the clinical immunotherapy of TNBC. |
format | Online Article Text |
id | pubmed-10234675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-102346752023-06-02 Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer Pan, Ting Zhang, Dinghu Wu, Xiaoxia Li, Zihou Zeng, Hui Xu, Xiawei Zhang, Chenguang He, Yiwei Gong, Yuanchuan Wang, Pin Mao, Quanliang Yao, Junlie Lin, Jie Wu, Aiguo Shao, Guoliang APL Bioeng Articles The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment efficiency. Surface-enhanced Raman spectroscopy (SERS) and magnetic resonance (MR) imaging exhibit great potential for early TNBC diagnosis. SERS, an optical imaging mode, has the advantages of high detection sensitivity, good spatial resolution, and “fingerprint” spectral characteristics; however, the shallow detection penetration of SERS bioprobes limits its application in vivo. MR has the advantages of allowing deep penetration with no radiation; however, its spatial resolution needs to be improved. SERS and MR have complementary imaging features for tumor marker detection. In this study, gold nanorod and ultrasmall iron oxide nanoparticle composites were developed as dual-modal bioprobes for SERS-MRI to detect PD-L1 expression. Anti-PD-L1 (aPD-L1) was utilized to improve the targeting ability and specificity of PD-L1 expression detection. TNBC cells expressing PD-L1 were accurately detected via the SERS imaging mode in vitro, which can image at the single-cell level. In addition, bioprobe accumulation in PD-L1 expression-related tumor-bearing mice was simply and dynamically monitored and analyzed in vivo using MR and SERS. To the best of our knowledge, this is the first time a SERS-MRI dual-modal bioprobe combined with a PD-L1 antibody has been successfully used to detect PD-L1 expression in TNBC. This work paves the way for the design of high-performance bioprobe-based contrast agents for the clinical immunotherapy of TNBC. AIP Publishing LLC 2023-05-23 /pmc/articles/PMC10234675/ /pubmed/37274628 http://dx.doi.org/10.1063/5.0152846 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Articles Pan, Ting Zhang, Dinghu Wu, Xiaoxia Li, Zihou Zeng, Hui Xu, Xiawei Zhang, Chenguang He, Yiwei Gong, Yuanchuan Wang, Pin Mao, Quanliang Yao, Junlie Lin, Jie Wu, Aiguo Shao, Guoliang Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
title | Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
title_full | Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
title_fullStr | Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
title_full_unstemmed | Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
title_short | Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
title_sort | gold nanorods with iron oxide dual-modal bioprobes in sers-mri enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234675/ https://www.ncbi.nlm.nih.gov/pubmed/37274628 http://dx.doi.org/10.1063/5.0152846 |
work_keys_str_mv | AT panting goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT zhangdinghu goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT wuxiaoxia goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT lizihou goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT zenghui goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT xuxiawei goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT zhangchenguang goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT heyiwei goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT gongyuanchuan goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT wangpin goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT maoquanliang goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT yaojunlie goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT linjie goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT wuaiguo goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer AT shaoguoliang goldnanorodswithironoxidedualmodalbioprobesinsersmrienableaccurateprogrammedcelldeathligand1expressiondetectionintriplenegativebreastcancer |