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Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer

The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment effic...

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Autores principales: Pan, Ting, Zhang, Dinghu, Wu, Xiaoxia, Li, Zihou, Zeng, Hui, Xu, Xiawei, Zhang, Chenguang, He, Yiwei, Gong, Yuanchuan, Wang, Pin, Mao, Quanliang, Yao, Junlie, Lin, Jie, Wu, Aiguo, Shao, Guoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234675/
https://www.ncbi.nlm.nih.gov/pubmed/37274628
http://dx.doi.org/10.1063/5.0152846
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author Pan, Ting
Zhang, Dinghu
Wu, Xiaoxia
Li, Zihou
Zeng, Hui
Xu, Xiawei
Zhang, Chenguang
He, Yiwei
Gong, Yuanchuan
Wang, Pin
Mao, Quanliang
Yao, Junlie
Lin, Jie
Wu, Aiguo
Shao, Guoliang
author_facet Pan, Ting
Zhang, Dinghu
Wu, Xiaoxia
Li, Zihou
Zeng, Hui
Xu, Xiawei
Zhang, Chenguang
He, Yiwei
Gong, Yuanchuan
Wang, Pin
Mao, Quanliang
Yao, Junlie
Lin, Jie
Wu, Aiguo
Shao, Guoliang
author_sort Pan, Ting
collection PubMed
description The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment efficiency. Surface-enhanced Raman spectroscopy (SERS) and magnetic resonance (MR) imaging exhibit great potential for early TNBC diagnosis. SERS, an optical imaging mode, has the advantages of high detection sensitivity, good spatial resolution, and “fingerprint” spectral characteristics; however, the shallow detection penetration of SERS bioprobes limits its application in vivo. MR has the advantages of allowing deep penetration with no radiation; however, its spatial resolution needs to be improved. SERS and MR have complementary imaging features for tumor marker detection. In this study, gold nanorod and ultrasmall iron oxide nanoparticle composites were developed as dual-modal bioprobes for SERS-MRI to detect PD-L1 expression. Anti-PD-L1 (aPD-L1) was utilized to improve the targeting ability and specificity of PD-L1 expression detection. TNBC cells expressing PD-L1 were accurately detected via the SERS imaging mode in vitro, which can image at the single-cell level. In addition, bioprobe accumulation in PD-L1 expression-related tumor-bearing mice was simply and dynamically monitored and analyzed in vivo using MR and SERS. To the best of our knowledge, this is the first time a SERS-MRI dual-modal bioprobe combined with a PD-L1 antibody has been successfully used to detect PD-L1 expression in TNBC. This work paves the way for the design of high-performance bioprobe-based contrast agents for the clinical immunotherapy of TNBC.
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spelling pubmed-102346752023-06-02 Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer Pan, Ting Zhang, Dinghu Wu, Xiaoxia Li, Zihou Zeng, Hui Xu, Xiawei Zhang, Chenguang He, Yiwei Gong, Yuanchuan Wang, Pin Mao, Quanliang Yao, Junlie Lin, Jie Wu, Aiguo Shao, Guoliang APL Bioeng Articles The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment efficiency. Surface-enhanced Raman spectroscopy (SERS) and magnetic resonance (MR) imaging exhibit great potential for early TNBC diagnosis. SERS, an optical imaging mode, has the advantages of high detection sensitivity, good spatial resolution, and “fingerprint” spectral characteristics; however, the shallow detection penetration of SERS bioprobes limits its application in vivo. MR has the advantages of allowing deep penetration with no radiation; however, its spatial resolution needs to be improved. SERS and MR have complementary imaging features for tumor marker detection. In this study, gold nanorod and ultrasmall iron oxide nanoparticle composites were developed as dual-modal bioprobes for SERS-MRI to detect PD-L1 expression. Anti-PD-L1 (aPD-L1) was utilized to improve the targeting ability and specificity of PD-L1 expression detection. TNBC cells expressing PD-L1 were accurately detected via the SERS imaging mode in vitro, which can image at the single-cell level. In addition, bioprobe accumulation in PD-L1 expression-related tumor-bearing mice was simply and dynamically monitored and analyzed in vivo using MR and SERS. To the best of our knowledge, this is the first time a SERS-MRI dual-modal bioprobe combined with a PD-L1 antibody has been successfully used to detect PD-L1 expression in TNBC. This work paves the way for the design of high-performance bioprobe-based contrast agents for the clinical immunotherapy of TNBC. AIP Publishing LLC 2023-05-23 /pmc/articles/PMC10234675/ /pubmed/37274628 http://dx.doi.org/10.1063/5.0152846 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Articles
Pan, Ting
Zhang, Dinghu
Wu, Xiaoxia
Li, Zihou
Zeng, Hui
Xu, Xiawei
Zhang, Chenguang
He, Yiwei
Gong, Yuanchuan
Wang, Pin
Mao, Quanliang
Yao, Junlie
Lin, Jie
Wu, Aiguo
Shao, Guoliang
Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
title Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
title_full Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
title_fullStr Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
title_full_unstemmed Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
title_short Gold nanorods with iron oxide dual-modal bioprobes in SERS-MRI enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
title_sort gold nanorods with iron oxide dual-modal bioprobes in sers-mri enable accurate programmed cell death ligand-1 expression detection in triple-negative breast cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234675/
https://www.ncbi.nlm.nih.gov/pubmed/37274628
http://dx.doi.org/10.1063/5.0152846
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