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Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing
OBJECTIVES: Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile of circRN...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234779/ https://www.ncbi.nlm.nih.gov/pubmed/37137710 http://dx.doi.org/10.1111/1759-7714.14884 |
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author | Chen, Yongyang Han, Xiaoling Huang, Xiaobi Zhou, Honglian Yu, Hui Wang, Lihui Liu, Zijian Liu, Baiyang Huang, Jian Xiong, Yinghuan Huang, Jian Shao, Yang Zhu, Dongqin Liang, Zhu Yang, Zhixiong Su, Wenmei |
author_facet | Chen, Yongyang Han, Xiaoling Huang, Xiaobi Zhou, Honglian Yu, Hui Wang, Lihui Liu, Zijian Liu, Baiyang Huang, Jian Xiong, Yinghuan Huang, Jian Shao, Yang Zhu, Dongqin Liang, Zhu Yang, Zhixiong Su, Wenmei |
author_sort | Chen, Yongyang |
collection | PubMed |
description | OBJECTIVES: Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile of circRNA in lung adenocarcinoma (LUAD). METHODS: This study included 14 pairs of postoperative lung adenocarcinoma specimens, including cancer tissues and matched adjacent tissues. Second‐generation sequencing was applied to the specimens to determine the circRNA expression in them among the 5242 distinct circRNAs detected. RESULTS: We identified a total of 18 significantly dysregulated circRNAs in the LUAD tissues: upregulation in four and downregulation in 14. ROC (The receiver operating characteristic curve) further suggested that hsa_circ_0120106, has_circ_0007342, has_circ_0005937, and circRNA_0000826 could potentially be used as biomarkers in the diagnosis of LUAD. Furthermore, study of the circRNA–microRNA (miRNA)–messenger RNA (mRNA) revealed interactions between the 18 dysregulated circRNA and several cancer‐related miRNAs. Finally, a further Kyoto Encyclopedia of Genes and Genomes analysis showed that the cell cycle phase transition, p53 signaling pathway, AMP‐activated protein kinase (AMPK) relative signaling pathway, and so on were key putative pathways in the process of LUAD. CONCLUSIONS: These findings demonstrated the correlation between abnormality in circRNA expression and LUAD, which lays the foundation of making CircRNAs candidate biomarkers in the diagnosis of LUAD. |
format | Online Article Text |
id | pubmed-10234779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-102347792023-06-03 Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing Chen, Yongyang Han, Xiaoling Huang, Xiaobi Zhou, Honglian Yu, Hui Wang, Lihui Liu, Zijian Liu, Baiyang Huang, Jian Xiong, Yinghuan Huang, Jian Shao, Yang Zhu, Dongqin Liang, Zhu Yang, Zhixiong Su, Wenmei Thorac Cancer Original Articles OBJECTIVES: Aberrantly expressed circular RNAs (circRNAs) have been detected in many types of tumors. Hence, they are currently investigated as candidate biomarkers for diagnostic and potential targets for therapy in cancers. The objective of this study was to assess the expression profile of circRNA in lung adenocarcinoma (LUAD). METHODS: This study included 14 pairs of postoperative lung adenocarcinoma specimens, including cancer tissues and matched adjacent tissues. Second‐generation sequencing was applied to the specimens to determine the circRNA expression in them among the 5242 distinct circRNAs detected. RESULTS: We identified a total of 18 significantly dysregulated circRNAs in the LUAD tissues: upregulation in four and downregulation in 14. ROC (The receiver operating characteristic curve) further suggested that hsa_circ_0120106, has_circ_0007342, has_circ_0005937, and circRNA_0000826 could potentially be used as biomarkers in the diagnosis of LUAD. Furthermore, study of the circRNA–microRNA (miRNA)–messenger RNA (mRNA) revealed interactions between the 18 dysregulated circRNA and several cancer‐related miRNAs. Finally, a further Kyoto Encyclopedia of Genes and Genomes analysis showed that the cell cycle phase transition, p53 signaling pathway, AMP‐activated protein kinase (AMPK) relative signaling pathway, and so on were key putative pathways in the process of LUAD. CONCLUSIONS: These findings demonstrated the correlation between abnormality in circRNA expression and LUAD, which lays the foundation of making CircRNAs candidate biomarkers in the diagnosis of LUAD. John Wiley & Sons Australia, Ltd 2023-05-03 /pmc/articles/PMC10234779/ /pubmed/37137710 http://dx.doi.org/10.1111/1759-7714.14884 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Chen, Yongyang Han, Xiaoling Huang, Xiaobi Zhou, Honglian Yu, Hui Wang, Lihui Liu, Zijian Liu, Baiyang Huang, Jian Xiong, Yinghuan Huang, Jian Shao, Yang Zhu, Dongqin Liang, Zhu Yang, Zhixiong Su, Wenmei Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing |
title | Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing |
title_full | Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing |
title_fullStr | Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing |
title_full_unstemmed | Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing |
title_short | Circular RNA‐mediated ceRNA network was identified in human lung adenocarcinoma by high‐throughput sequencing |
title_sort | circular rna‐mediated cerna network was identified in human lung adenocarcinoma by high‐throughput sequencing |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234779/ https://www.ncbi.nlm.nih.gov/pubmed/37137710 http://dx.doi.org/10.1111/1759-7714.14884 |
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