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CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis

BACKGROUND: Circ‐ZKSCAN1 has been found to accelerate non‐small cell lung cancer (NSCLC) progression; however, the role and mechanism of circ‐ZKSCAN1 in lung adenocarcinoma (LUAD) cisplatin (DDP) resistance remain unclear. METHODS: Levels of genes and proteins were examined using qRT‐PCR. Functional...

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Detalles Bibliográficos
Autores principales: Yu, Nanbin, Gong, Huaijun, Chen, Wei, Peng, Weixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234786/
https://www.ncbi.nlm.nih.gov/pubmed/37105934
http://dx.doi.org/10.1111/1759-7714.14889
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author Yu, Nanbin
Gong, Huaijun
Chen, Wei
Peng, Weixing
author_facet Yu, Nanbin
Gong, Huaijun
Chen, Wei
Peng, Weixing
author_sort Yu, Nanbin
collection PubMed
description BACKGROUND: Circ‐ZKSCAN1 has been found to accelerate non‐small cell lung cancer (NSCLC) progression; however, the role and mechanism of circ‐ZKSCAN1 in lung adenocarcinoma (LUAD) cisplatin (DDP) resistance remain unclear. METHODS: Levels of genes and proteins were examined using qRT‐PCR. Functional experiments were performed using CCK‐8 assay, flow cytometry, transwell assay and xenograft model assay, respectively. Glucose metabolism was calculated by detecting glucose consumption, lactate production, ATP and HK‐2 levels. The interaction between miR‐185‐5p and circ‐ZKSCAN1 or transgelin 2 (TAGLN2) was validated by dual‐luciferase reporter assay. RESULTS: Circ‐ZKSCAN1 was highly expressed in DDP‐resistant LUAD tissues and cell lines, and circ‐ZKSCAN1 knockdown weakened DDP resistance and suppressed cell viability, migration, invasion, and glycolysis in LUAD. Circ‐ZKSCAN1 acted as a sponge of miR‐185‐5p, and the regulatory effects of circ‐ZKSCAN1 knockdown on LUAD were reversed by miR‐185‐5p downregulation. Meanwhile, miR‐185‐5p directly targeted TAGLN2, and performed anticancer effects by regulating TAGLN2. Importantly, silencing of circ‐ZKSCAN1 hindered tumor growth and promoted DDP sensitivity in vivo via regulating miR‐185‐5p and TAGLN2. CONCLUSION: Circ‐ZKSCAN1 promoted LUAD tumorigenesis and DDP resistance by regulating miR‐185‐5p/TAGLN2 axis.
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spelling pubmed-102347862023-06-03 CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis Yu, Nanbin Gong, Huaijun Chen, Wei Peng, Weixing Thorac Cancer Original Articles BACKGROUND: Circ‐ZKSCAN1 has been found to accelerate non‐small cell lung cancer (NSCLC) progression; however, the role and mechanism of circ‐ZKSCAN1 in lung adenocarcinoma (LUAD) cisplatin (DDP) resistance remain unclear. METHODS: Levels of genes and proteins were examined using qRT‐PCR. Functional experiments were performed using CCK‐8 assay, flow cytometry, transwell assay and xenograft model assay, respectively. Glucose metabolism was calculated by detecting glucose consumption, lactate production, ATP and HK‐2 levels. The interaction between miR‐185‐5p and circ‐ZKSCAN1 or transgelin 2 (TAGLN2) was validated by dual‐luciferase reporter assay. RESULTS: Circ‐ZKSCAN1 was highly expressed in DDP‐resistant LUAD tissues and cell lines, and circ‐ZKSCAN1 knockdown weakened DDP resistance and suppressed cell viability, migration, invasion, and glycolysis in LUAD. Circ‐ZKSCAN1 acted as a sponge of miR‐185‐5p, and the regulatory effects of circ‐ZKSCAN1 knockdown on LUAD were reversed by miR‐185‐5p downregulation. Meanwhile, miR‐185‐5p directly targeted TAGLN2, and performed anticancer effects by regulating TAGLN2. Importantly, silencing of circ‐ZKSCAN1 hindered tumor growth and promoted DDP sensitivity in vivo via regulating miR‐185‐5p and TAGLN2. CONCLUSION: Circ‐ZKSCAN1 promoted LUAD tumorigenesis and DDP resistance by regulating miR‐185‐5p/TAGLN2 axis. John Wiley & Sons Australia, Ltd 2023-04-27 /pmc/articles/PMC10234786/ /pubmed/37105934 http://dx.doi.org/10.1111/1759-7714.14889 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Yu, Nanbin
Gong, Huaijun
Chen, Wei
Peng, Weixing
CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis
title CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis
title_full CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis
title_fullStr CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis
title_full_unstemmed CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis
title_short CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR‐185‐5p/TAGLN2 axis
title_sort circrna zkscan1 promotes lung adenocarcinoma progression by mir‐185‐5p/tagln2 axis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234786/
https://www.ncbi.nlm.nih.gov/pubmed/37105934
http://dx.doi.org/10.1111/1759-7714.14889
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