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Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork

Hybridization between milky stork (Mycteria cinerea) and painted stork (M. leucocephala) occurs frequently in captivity. Dusit Zoo is a captive breeding facility where storks with phenotypically ambiguous patterns have recently been observed, and their status remaining inconclusive. Here, we used a...

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Autores principales: Kaminsin, Damisa, Warrit, Natapot, Sankhom, Rangsinee, Piamsanga, Krittee, Sanannu, Saowaphang, Baicharoen, Sudarath, Wiwegweaw, Amporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235083/
https://www.ncbi.nlm.nih.gov/pubmed/37264039
http://dx.doi.org/10.1038/s41598-023-35566-x
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author Kaminsin, Damisa
Warrit, Natapot
Sankhom, Rangsinee
Piamsanga, Krittee
Sanannu, Saowaphang
Baicharoen, Sudarath
Wiwegweaw, Amporn
author_facet Kaminsin, Damisa
Warrit, Natapot
Sankhom, Rangsinee
Piamsanga, Krittee
Sanannu, Saowaphang
Baicharoen, Sudarath
Wiwegweaw, Amporn
author_sort Kaminsin, Damisa
collection PubMed
description Hybridization between milky stork (Mycteria cinerea) and painted stork (M. leucocephala) occurs frequently in captivity. Dusit Zoo is a captive breeding facility where storks with phenotypically ambiguous patterns have recently been observed, and their status remaining inconclusive. Here, we used a combination of phenotypic characters and genetic markers (cytochrome b and 14 microsatellite markers) to distinguish and identify hybrids from the two parental species (n = 114). Haplotype analysis revealed asymmetric mtDNA introgression from M. cinerea to M. leucocephala, with twelve morphologically classified M. leucocephala individuals carrying heterospecific mtDNA. Comprehensive biparental genetic assessments identified 33% of all three genetic clusters as admixed individuals, of which most were either F2 hybrids, backcrosses with M. leucocephala, or hybrids of unknown generation, implying weak premating isolation with the absence of intrinsic postzygotic isolation between parentals. Morphological analysis demonstrated that the absence or indistinctness of a black bar across the breast is the most noticeable trait to identify these hybrids. The endangered M. cinerea was found to have genomic contamination from M. leucocephala and vice versa, with at least 41 hybrid individuals being identified. These findings provide critical information for detecting hybrids and identifying suitable breeding stocks with genetic purity for future reintroduction and conservation management.
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spelling pubmed-102350832023-06-03 Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork Kaminsin, Damisa Warrit, Natapot Sankhom, Rangsinee Piamsanga, Krittee Sanannu, Saowaphang Baicharoen, Sudarath Wiwegweaw, Amporn Sci Rep Article Hybridization between milky stork (Mycteria cinerea) and painted stork (M. leucocephala) occurs frequently in captivity. Dusit Zoo is a captive breeding facility where storks with phenotypically ambiguous patterns have recently been observed, and their status remaining inconclusive. Here, we used a combination of phenotypic characters and genetic markers (cytochrome b and 14 microsatellite markers) to distinguish and identify hybrids from the two parental species (n = 114). Haplotype analysis revealed asymmetric mtDNA introgression from M. cinerea to M. leucocephala, with twelve morphologically classified M. leucocephala individuals carrying heterospecific mtDNA. Comprehensive biparental genetic assessments identified 33% of all three genetic clusters as admixed individuals, of which most were either F2 hybrids, backcrosses with M. leucocephala, or hybrids of unknown generation, implying weak premating isolation with the absence of intrinsic postzygotic isolation between parentals. Morphological analysis demonstrated that the absence or indistinctness of a black bar across the breast is the most noticeable trait to identify these hybrids. The endangered M. cinerea was found to have genomic contamination from M. leucocephala and vice versa, with at least 41 hybrid individuals being identified. These findings provide critical information for detecting hybrids and identifying suitable breeding stocks with genetic purity for future reintroduction and conservation management. Nature Publishing Group UK 2023-06-01 /pmc/articles/PMC10235083/ /pubmed/37264039 http://dx.doi.org/10.1038/s41598-023-35566-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kaminsin, Damisa
Warrit, Natapot
Sankhom, Rangsinee
Piamsanga, Krittee
Sanannu, Saowaphang
Baicharoen, Sudarath
Wiwegweaw, Amporn
Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
title Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
title_full Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
title_fullStr Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
title_full_unstemmed Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
title_short Detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
title_sort detecting introgressive hybridization to maintain genetic integrity in endangered large waterbird: a case study in milky stork
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235083/
https://www.ncbi.nlm.nih.gov/pubmed/37264039
http://dx.doi.org/10.1038/s41598-023-35566-x
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