Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece

Using high-throughput sequencing, we identified a novel carlavirus sequence in a 28-year-old ‘Kotsifali’ grapevine sample collected in Heraklion (Crete, Greece). Using RT-PCR and 5'/3' RACE together with Sanger sequencing, the complete genome sequence of 8299 nt was confirmed and found to...

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Detalles Bibliográficos
Autores principales: Katsarou, Konstantina, Andronis, Christos, James, Anthony, Euthymiou, Kostas, Kryovrysanaki, Nikoleta, Pappi, Polykseni G., Kalantidis, Kriton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2023
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235145/
https://www.ncbi.nlm.nih.gov/pubmed/37261601
http://dx.doi.org/10.1007/s00705-023-05795-6
Descripción
Sumario:Using high-throughput sequencing, we identified a novel carlavirus sequence in a 28-year-old ‘Kotsifali’ grapevine sample collected in Heraklion (Crete, Greece). Using RT-PCR and 5'/3' RACE together with Sanger sequencing, the complete genome sequence of 8299 nt was confirmed and found to contain five open reading frames (ORFs) but to lack an ORF6, which is present in some members of the genus Carlavirus. The novel sequence is most similar to those of two carlaviruses infecting caper, and taking into account the ICTV nomenclature, we propose the name "grapevine carlavirus 1" for this new virus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05795-6.

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