Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece

Using high-throughput sequencing, we identified a novel carlavirus sequence in a 28-year-old ‘Kotsifali’ grapevine sample collected in Heraklion (Crete, Greece). Using RT-PCR and 5'/3' RACE together with Sanger sequencing, the complete genome sequence of 8299 nt was confirmed and found to...

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Autores principales: Katsarou, Konstantina, Andronis, Christos, James, Anthony, Euthymiou, Kostas, Kryovrysanaki, Nikoleta, Pappi, Polykseni G., Kalantidis, Kriton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2023
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235145/
https://www.ncbi.nlm.nih.gov/pubmed/37261601
http://dx.doi.org/10.1007/s00705-023-05795-6
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author Katsarou, Konstantina
Andronis, Christos
James, Anthony
Euthymiou, Kostas
Kryovrysanaki, Nikoleta
Pappi, Polykseni G.
Kalantidis, Kriton
author_facet Katsarou, Konstantina
Andronis, Christos
James, Anthony
Euthymiou, Kostas
Kryovrysanaki, Nikoleta
Pappi, Polykseni G.
Kalantidis, Kriton
author_sort Katsarou, Konstantina
collection PubMed
description Using high-throughput sequencing, we identified a novel carlavirus sequence in a 28-year-old ‘Kotsifali’ grapevine sample collected in Heraklion (Crete, Greece). Using RT-PCR and 5'/3' RACE together with Sanger sequencing, the complete genome sequence of 8299 nt was confirmed and found to contain five open reading frames (ORFs) but to lack an ORF6, which is present in some members of the genus Carlavirus. The novel sequence is most similar to those of two carlaviruses infecting caper, and taking into account the ICTV nomenclature, we propose the name "grapevine carlavirus 1" for this new virus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05795-6.
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spelling pubmed-102351452023-06-03 Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece Katsarou, Konstantina Andronis, Christos James, Anthony Euthymiou, Kostas Kryovrysanaki, Nikoleta Pappi, Polykseni G. Kalantidis, Kriton Arch Virol Annotated Sequence Record Using high-throughput sequencing, we identified a novel carlavirus sequence in a 28-year-old ‘Kotsifali’ grapevine sample collected in Heraklion (Crete, Greece). Using RT-PCR and 5'/3' RACE together with Sanger sequencing, the complete genome sequence of 8299 nt was confirmed and found to contain five open reading frames (ORFs) but to lack an ORF6, which is present in some members of the genus Carlavirus. The novel sequence is most similar to those of two carlaviruses infecting caper, and taking into account the ICTV nomenclature, we propose the name "grapevine carlavirus 1" for this new virus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05795-6. Springer Vienna 2023-06-01 2023 /pmc/articles/PMC10235145/ /pubmed/37261601 http://dx.doi.org/10.1007/s00705-023-05795-6 Text en © The Author(s) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Annotated Sequence Record
Katsarou, Konstantina
Andronis, Christos
James, Anthony
Euthymiou, Kostas
Kryovrysanaki, Nikoleta
Pappi, Polykseni G.
Kalantidis, Kriton
Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece
title Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece
title_full Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece
title_fullStr Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece
title_full_unstemmed Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece
title_short Complete genome sequence of a carlavirus identified in grapevine (Vitis sp) in Greece
title_sort complete genome sequence of a carlavirus identified in grapevine (vitis sp) in greece
topic Annotated Sequence Record
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235145/
https://www.ncbi.nlm.nih.gov/pubmed/37261601
http://dx.doi.org/10.1007/s00705-023-05795-6
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