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Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread

Equine herpesvirus type 1 (EHV-1) is a highly transmissible pathogen that leads to a variety of clinical disease outcomes in infected horses. A major sequela that can occur after an EHV-1 infection is a neurological disease termed equine herpesvirus myeloencephalopathy (EHM). Clinical manifestations...

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Autores principales: Black, Jeanette B., Frampton, Arthur R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235532/
https://www.ncbi.nlm.nih.gov/pubmed/37275614
http://dx.doi.org/10.3389/fvets.2023.1165917
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author Black, Jeanette B.
Frampton, Arthur R.
author_facet Black, Jeanette B.
Frampton, Arthur R.
author_sort Black, Jeanette B.
collection PubMed
description Equine herpesvirus type 1 (EHV-1) is a highly transmissible pathogen that leads to a variety of clinical disease outcomes in infected horses. A major sequela that can occur after an EHV-1 infection is a neurological disease termed equine herpesvirus myeloencephalopathy (EHM). Clinical manifestations of EHM include fever, ataxia, incontinence, and partial to full paralysis, which may ultimately lead to the euthanization of the infected horse. To develop an effective treatment strategy for EHM, it is critical that the specific virus–host interactions that lead to EHM be investigated so that safe and effective therapeutic interventions can be developed and delivered. In this study, we examined the ability of four non-steroidal anti-inflammatory drugs (NSAIDs), a steroidal anti-inflammatory drug (dexamethasone), a Rho-kinase (ROCK) inhibitor, and a JAK/STAT inhibitor (AG490) to reduce EHV-1 virus yields and cell-to-cell spread. We show that the NSAID, flunixin meglumine (FM), and the JAK/STAT inhibitor, AG490, significantly reduced virus yields in endothelial and epithelial cell lines, and this inhibition was similar for two neurologic and two non-neurologic EHV-1 strains. In addition to reducing virus yields, AG490 and FM also significantly reduced the ability of EHV-1 to spread laterally from cell to cell.
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spelling pubmed-102355322023-06-03 Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread Black, Jeanette B. Frampton, Arthur R. Front Vet Sci Veterinary Science Equine herpesvirus type 1 (EHV-1) is a highly transmissible pathogen that leads to a variety of clinical disease outcomes in infected horses. A major sequela that can occur after an EHV-1 infection is a neurological disease termed equine herpesvirus myeloencephalopathy (EHM). Clinical manifestations of EHM include fever, ataxia, incontinence, and partial to full paralysis, which may ultimately lead to the euthanization of the infected horse. To develop an effective treatment strategy for EHM, it is critical that the specific virus–host interactions that lead to EHM be investigated so that safe and effective therapeutic interventions can be developed and delivered. In this study, we examined the ability of four non-steroidal anti-inflammatory drugs (NSAIDs), a steroidal anti-inflammatory drug (dexamethasone), a Rho-kinase (ROCK) inhibitor, and a JAK/STAT inhibitor (AG490) to reduce EHV-1 virus yields and cell-to-cell spread. We show that the NSAID, flunixin meglumine (FM), and the JAK/STAT inhibitor, AG490, significantly reduced virus yields in endothelial and epithelial cell lines, and this inhibition was similar for two neurologic and two non-neurologic EHV-1 strains. In addition to reducing virus yields, AG490 and FM also significantly reduced the ability of EHV-1 to spread laterally from cell to cell. Frontiers Media S.A. 2023-05-19 /pmc/articles/PMC10235532/ /pubmed/37275614 http://dx.doi.org/10.3389/fvets.2023.1165917 Text en Copyright © 2023 Black and Frampton. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Black, Jeanette B.
Frampton, Arthur R.
Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
title Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
title_full Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
title_fullStr Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
title_full_unstemmed Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
title_short Anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
title_sort anti-inflammatory compounds reduce equine herpesvirus type 1 replication and cell-to-cell spread
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235532/
https://www.ncbi.nlm.nih.gov/pubmed/37275614
http://dx.doi.org/10.3389/fvets.2023.1165917
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