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Staphylococcus aureus δ-toxin present on skin promotes the development of food allergy in a murine model

BACKGROUND: Patients with food allergy often suffer from atopic dermatitis, in which Staphylococcus aureus colonization is frequently observed. Staphylococcus aureus δ-toxin activates mast cells and promotes T helper 2 type skin inflammation in the tape-stripped murine skin. However, the physiologic...

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Detalles Bibliográficos
Autores principales: Yamada, Hiromichi, Kaitani, Ayako, Izawa, Kumi, Ando, Tomoaki, Kamei, Anna, Uchida, Shino, Maehara, Akie, Kojima, Mayuki, Yamamoto, Risa, Wang, Hexing, Nagamine, Masakazu, Maeda, Keiko, Uchida, Koichiro, Nakano, Nobuhiro, Ohtsuka, Yoshikazu, Ogawa, Hideoki, Okumura, Ko, Shimizu, Toshiaki, Kitaura, Jiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235538/
https://www.ncbi.nlm.nih.gov/pubmed/37275864
http://dx.doi.org/10.3389/fimmu.2023.1173069
Descripción
Sumario:BACKGROUND: Patients with food allergy often suffer from atopic dermatitis, in which Staphylococcus aureus colonization is frequently observed. Staphylococcus aureus δ-toxin activates mast cells and promotes T helper 2 type skin inflammation in the tape-stripped murine skin. However, the physiological effects of δ-toxin present on the steady-state skin remain unknown. We aimed to investigate whether δ-toxin present on the steady-state skin impacts the development of food allergy. MATERIAL AND METHODS: The non-tape-stripped skins of wild-type, Kit(W-sh/W-sh) , or ST2-deficient mice were treated with ovalbumin (OVA) with or without δ-toxin before intragastric administration of OVA. The frequency of diarrhea, numbers of jejunum or skin mast cells, and serum levels of OVA-specific IgE were measured. Conventional dendritic cell 2 (cDC2) in skin and lymph nodes (LN) were analyzed. The cytokine levels in the skin tissues or culture supernatants of δ-toxin-stimulated murine keratinocytes were measured. Anti-IL-1α antibody-pretreated mice were analyzed. RESULTS: Stimulation with δ-toxin induced the release of IL-1α, but not IL-33, in murine keratinocytes. Epicutaneous treatment with OVA and δ-toxin induced the local production of IL-1α. This treatment induced the translocation of OVA-loaded cDC2 from skin to draining LN and OVA-specific IgE production, independently of mast cells and ST2. This resulted in OVA-administered food allergic responses. In these models, pretreatment with anti-IL-1α antibody inhibited the cDC2 activation and OVA-specific IgE production, thereby dampening food allergic responses. CONCLUSION: Even without tape stripping, δ-toxin present on skin enhances epicutaneous sensitization to food allergen in an IL-1α-dependent manner, thereby promoting the development of food allergy.