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Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels

Ethanol abuse is a risk factor for the development of pneumonia caused by Streptococcus pneumoniae, a critical pathogen for public health. The aim of this article was to investigate the inflammatory mechanisms involved in pneumococcal pneumonia that may be associated with chronic ethanol exposure. M...

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Autores principales: Martins, Flávia Rayssa Braga, de Oliveira, Maycon Douglas, Souza, Jéssica Amanda Marques, Queiroz-Junior, Celso Martins, Lobo, Francisco Pereira, Teixeira, Mauro Martins, Malacco, Nathalia Luisa, Soriani, Frederico Marianetti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235596/
https://www.ncbi.nlm.nih.gov/pubmed/37275853
http://dx.doi.org/10.3389/fimmu.2023.1175275
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author Martins, Flávia Rayssa Braga
de Oliveira, Maycon Douglas
Souza, Jéssica Amanda Marques
Queiroz-Junior, Celso Martins
Lobo, Francisco Pereira
Teixeira, Mauro Martins
Malacco, Nathalia Luisa
Soriani, Frederico Marianetti
author_facet Martins, Flávia Rayssa Braga
de Oliveira, Maycon Douglas
Souza, Jéssica Amanda Marques
Queiroz-Junior, Celso Martins
Lobo, Francisco Pereira
Teixeira, Mauro Martins
Malacco, Nathalia Luisa
Soriani, Frederico Marianetti
author_sort Martins, Flávia Rayssa Braga
collection PubMed
description Ethanol abuse is a risk factor for the development of pneumonia caused by Streptococcus pneumoniae, a critical pathogen for public health. The aim of this article was to investigate the inflammatory mechanisms involved in pneumococcal pneumonia that may be associated with chronic ethanol exposure. Male C57BL6/J-Unib mice were exposed to 20% (v/v) ethanol for twelve weeks and intranasally infected with 5x10(4) CFU of S. pneumoniae. Twenty-four hours after infection, lungs, bronchoalveolar lavage and blood samples were obtained to assess the consequences of chronic ethanol exposure during infection. Alcohol-fed mice showed increased production of nitric oxide and CXCL1 in alveoli and plasma during pneumococcal pneumonia. Beside this, ethanol-treated mice exhibited a decrease in leukocyte infiltration into the alveoli and reduced frequency of severe lung inflammation, which was associated with an increase in bacterial load. Curiously, no changes were observed in survival after infection. Taken together, these results demonstrate that chronic ethanol exposure alters the inflammatory response during S. pneumoniae lung infection in mice with a reduction in the inflammatory infiltrate even in the presence of higher levels of the chemoattractant CXCL1.
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spelling pubmed-102355962023-06-03 Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels Martins, Flávia Rayssa Braga de Oliveira, Maycon Douglas Souza, Jéssica Amanda Marques Queiroz-Junior, Celso Martins Lobo, Francisco Pereira Teixeira, Mauro Martins Malacco, Nathalia Luisa Soriani, Frederico Marianetti Front Immunol Immunology Ethanol abuse is a risk factor for the development of pneumonia caused by Streptococcus pneumoniae, a critical pathogen for public health. The aim of this article was to investigate the inflammatory mechanisms involved in pneumococcal pneumonia that may be associated with chronic ethanol exposure. Male C57BL6/J-Unib mice were exposed to 20% (v/v) ethanol for twelve weeks and intranasally infected with 5x10(4) CFU of S. pneumoniae. Twenty-four hours after infection, lungs, bronchoalveolar lavage and blood samples were obtained to assess the consequences of chronic ethanol exposure during infection. Alcohol-fed mice showed increased production of nitric oxide and CXCL1 in alveoli and plasma during pneumococcal pneumonia. Beside this, ethanol-treated mice exhibited a decrease in leukocyte infiltration into the alveoli and reduced frequency of severe lung inflammation, which was associated with an increase in bacterial load. Curiously, no changes were observed in survival after infection. Taken together, these results demonstrate that chronic ethanol exposure alters the inflammatory response during S. pneumoniae lung infection in mice with a reduction in the inflammatory infiltrate even in the presence of higher levels of the chemoattractant CXCL1. Frontiers Media S.A. 2023-05-19 /pmc/articles/PMC10235596/ /pubmed/37275853 http://dx.doi.org/10.3389/fimmu.2023.1175275 Text en Copyright © 2023 Martins, de Oliveira, Souza, Queiroz-Junior, Lobo, Teixeira, Malacco and Soriani https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Martins, Flávia Rayssa Braga
de Oliveira, Maycon Douglas
Souza, Jéssica Amanda Marques
Queiroz-Junior, Celso Martins
Lobo, Francisco Pereira
Teixeira, Mauro Martins
Malacco, Nathalia Luisa
Soriani, Frederico Marianetti
Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels
title Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels
title_full Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels
title_fullStr Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels
title_full_unstemmed Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels
title_short Chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased CXCL1 and nitric oxide levels
title_sort chronic ethanol exposure impairs alveolar leukocyte infiltration during pneumococcal pneumonia, leading to an increased bacterial burden despite increased cxcl1 and nitric oxide levels
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235596/
https://www.ncbi.nlm.nih.gov/pubmed/37275853
http://dx.doi.org/10.3389/fimmu.2023.1175275
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