Cargando…

Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize

Plant growth-promoting bacteria (PGPB) are a source of nutrient supply, stimulate plant growth, and even act in the biocontrol of phytopathogens. However, these phenotypic traits have rarely been explored in culturable bacteria from native maize landraces. In this study, synthetic microbial communit...

Descripción completa

Detalles Bibliográficos
Autores principales: De la Vega-Camarillo, Esaú, Sotelo-Aguilar, Josimar, Rios-Galicia, Bibiana, Mercado-Flores, Yuridia, Arteaga-Garibay, Ramón, Villa-Tanaca, Lourdes, Hernández-Rodríguez, César
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235630/
https://www.ncbi.nlm.nih.gov/pubmed/37275168
http://dx.doi.org/10.3389/fmicb.2023.1167839
_version_ 1785052729440206848
author De la Vega-Camarillo, Esaú
Sotelo-Aguilar, Josimar
Rios-Galicia, Bibiana
Mercado-Flores, Yuridia
Arteaga-Garibay, Ramón
Villa-Tanaca, Lourdes
Hernández-Rodríguez, César
author_facet De la Vega-Camarillo, Esaú
Sotelo-Aguilar, Josimar
Rios-Galicia, Bibiana
Mercado-Flores, Yuridia
Arteaga-Garibay, Ramón
Villa-Tanaca, Lourdes
Hernández-Rodríguez, César
author_sort De la Vega-Camarillo, Esaú
collection PubMed
description Plant growth-promoting bacteria (PGPB) are a source of nutrient supply, stimulate plant growth, and even act in the biocontrol of phytopathogens. However, these phenotypic traits have rarely been explored in culturable bacteria from native maize landraces. In this study, synthetic microbial communities (SynCom) were assembled with a set of PGPB isolated from the Jala maize landrace, some of them with additional abilities for the biocontrol of phytopathogenic fungi and the stimulation of plant-induced systemic resistance (ISR). Three SynCom were designed considering the phenotypic traits of bacterial strains, including Achromobacter xylosoxidans Z2K8, Burkholderia sp. Z1AL11, Klebsiella variicola R3J3HD7, Kosakonia pseudosacchari Z2WD1, Pantoea ananatis E2HD8, Pantoea sp. E2AD2, Phytobacter diazotrophicus Z2WL1, Pseudomonas protegens E1BL2, and P. protegens E2HL9. Plant growth promotion in gnotobiotic and greenhouse seedlings assays was performed with Conejo landrace; meanwhile, open field tests were carried out on hybrid CPL9105W maize. In all experimental models, a significant promotion of plant growth was observed. In gnotobiotic assays, the roots and shoot length of the maize seedlings increased 4.2 and 3.0 times, respectively, compared to the untreated control. Similarly, the sizes and weights of the roots and shoots of the plants increased significantly in the greenhouse assays. In the open field assay performed with hybrid CPL9105W maize, the yield increased from 11 tons/ha for the control to 16 tons/ha inoculated with SynCom 3. In addition, the incidence of rust fungal infections decreased significantly from 12.5% in the control to 8% in the treatment with SynCom 3. All SynCom designs promoted the growth of maize in all assays. However, SynCom 3 formulated with A. xylosoxidans Z2K8, Burkholderia sp. Z1AL11, K. variicola R3J3HD7, P. ananatis E2HD8, P. diazotrophicus Z2WL1, and P. protegens E1BL2 displayed the best results for promoting plant growth, their yield, and the inhibition of fungal rust. This study demonstrated the biotechnological eco-friendly plant growth-promoting potential of SynCom assemblies with culturable bacteria from native maize landraces for more sustainable and economic agriculture.
format Online
Article
Text
id pubmed-10235630
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102356302023-06-03 Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize De la Vega-Camarillo, Esaú Sotelo-Aguilar, Josimar Rios-Galicia, Bibiana Mercado-Flores, Yuridia Arteaga-Garibay, Ramón Villa-Tanaca, Lourdes Hernández-Rodríguez, César Front Microbiol Microbiology Plant growth-promoting bacteria (PGPB) are a source of nutrient supply, stimulate plant growth, and even act in the biocontrol of phytopathogens. However, these phenotypic traits have rarely been explored in culturable bacteria from native maize landraces. In this study, synthetic microbial communities (SynCom) were assembled with a set of PGPB isolated from the Jala maize landrace, some of them with additional abilities for the biocontrol of phytopathogenic fungi and the stimulation of plant-induced systemic resistance (ISR). Three SynCom were designed considering the phenotypic traits of bacterial strains, including Achromobacter xylosoxidans Z2K8, Burkholderia sp. Z1AL11, Klebsiella variicola R3J3HD7, Kosakonia pseudosacchari Z2WD1, Pantoea ananatis E2HD8, Pantoea sp. E2AD2, Phytobacter diazotrophicus Z2WL1, Pseudomonas protegens E1BL2, and P. protegens E2HL9. Plant growth promotion in gnotobiotic and greenhouse seedlings assays was performed with Conejo landrace; meanwhile, open field tests were carried out on hybrid CPL9105W maize. In all experimental models, a significant promotion of plant growth was observed. In gnotobiotic assays, the roots and shoot length of the maize seedlings increased 4.2 and 3.0 times, respectively, compared to the untreated control. Similarly, the sizes and weights of the roots and shoots of the plants increased significantly in the greenhouse assays. In the open field assay performed with hybrid CPL9105W maize, the yield increased from 11 tons/ha for the control to 16 tons/ha inoculated with SynCom 3. In addition, the incidence of rust fungal infections decreased significantly from 12.5% in the control to 8% in the treatment with SynCom 3. All SynCom designs promoted the growth of maize in all assays. However, SynCom 3 formulated with A. xylosoxidans Z2K8, Burkholderia sp. Z1AL11, K. variicola R3J3HD7, P. ananatis E2HD8, P. diazotrophicus Z2WL1, and P. protegens E1BL2 displayed the best results for promoting plant growth, their yield, and the inhibition of fungal rust. This study demonstrated the biotechnological eco-friendly plant growth-promoting potential of SynCom assemblies with culturable bacteria from native maize landraces for more sustainable and economic agriculture. Frontiers Media S.A. 2023-05-19 /pmc/articles/PMC10235630/ /pubmed/37275168 http://dx.doi.org/10.3389/fmicb.2023.1167839 Text en Copyright © 2023 De la Vega-Camarillo, Sotelo-Aguilar, Rios-Galicia, Mercado-Flores, Arteaga-Garibay, Villa-Tanaca and Hernández-Rodríguez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
De la Vega-Camarillo, Esaú
Sotelo-Aguilar, Josimar
Rios-Galicia, Bibiana
Mercado-Flores, Yuridia
Arteaga-Garibay, Ramón
Villa-Tanaca, Lourdes
Hernández-Rodríguez, César
Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
title Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
title_full Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
title_fullStr Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
title_full_unstemmed Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
title_short Promotion of the growth and yield of Zea mays by synthetic microbial communities from Jala maize
title_sort promotion of the growth and yield of zea mays by synthetic microbial communities from jala maize
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235630/
https://www.ncbi.nlm.nih.gov/pubmed/37275168
http://dx.doi.org/10.3389/fmicb.2023.1167839
work_keys_str_mv AT delavegacamarilloesau promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize
AT soteloaguilarjosimar promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize
AT riosgaliciabibiana promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize
AT mercadofloresyuridia promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize
AT arteagagaribayramon promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize
AT villatanacalourdes promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize
AT hernandezrodriguezcesar promotionofthegrowthandyieldofzeamaysbysyntheticmicrobialcommunitiesfromjalamaize