Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research

BACKGROUND: Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system. METHODS: Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibe...

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Detalles Bibliográficos
Autores principales: Graumans, Wouter, Schinkel, Michelle, van Gemert, Geert-Jan, Spitzen, Jeroen, Bousema, Teun, Miesen, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235806/
https://www.ncbi.nlm.nih.gov/pubmed/36637101
http://dx.doi.org/10.1093/trstmh/trac135
Descripción
Sumario:BACKGROUND: Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system. METHODS: Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts. RESULTS: While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane. CONCLUSIONS: Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.

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