Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research

BACKGROUND: Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system. METHODS: Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibe...

Descripción completa

Detalles Bibliográficos
Autores principales: Graumans, Wouter, Schinkel, Michelle, van Gemert, Geert-Jan, Spitzen, Jeroen, Bousema, Teun, Miesen, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235806/
https://www.ncbi.nlm.nih.gov/pubmed/36637101
http://dx.doi.org/10.1093/trstmh/trac135
_version_ 1785052772431822848
author Graumans, Wouter
Schinkel, Michelle
van Gemert, Geert-Jan
Spitzen, Jeroen
Bousema, Teun
Miesen, Pascal
author_facet Graumans, Wouter
Schinkel, Michelle
van Gemert, Geert-Jan
Spitzen, Jeroen
Bousema, Teun
Miesen, Pascal
author_sort Graumans, Wouter
collection PubMed
description BACKGROUND: Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system. METHODS: Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts. RESULTS: While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane. CONCLUSIONS: Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.
format Online
Article
Text
id pubmed-10235806
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-102358062023-06-03 Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research Graumans, Wouter Schinkel, Michelle van Gemert, Geert-Jan Spitzen, Jeroen Bousema, Teun Miesen, Pascal Trans R Soc Trop Med Hyg Short Communication BACKGROUND: Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system. METHODS: Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts. RESULTS: While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane. CONCLUSIONS: Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research. Oxford University Press 2023-01-13 /pmc/articles/PMC10235806/ /pubmed/36637101 http://dx.doi.org/10.1093/trstmh/trac135 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Graumans, Wouter
Schinkel, Michelle
van Gemert, Geert-Jan
Spitzen, Jeroen
Bousema, Teun
Miesen, Pascal
Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research
title Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research
title_full Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research
title_fullStr Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research
title_full_unstemmed Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research
title_short Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research
title_sort comparative analysis of glass and hemotek membrane feeding systems for malaria transmission research
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235806/
https://www.ncbi.nlm.nih.gov/pubmed/36637101
http://dx.doi.org/10.1093/trstmh/trac135
work_keys_str_mv AT graumanswouter comparativeanalysisofglassandhemotekmembranefeedingsystemsformalariatransmissionresearch
AT schinkelmichelle comparativeanalysisofglassandhemotekmembranefeedingsystemsformalariatransmissionresearch
AT vangemertgeertjan comparativeanalysisofglassandhemotekmembranefeedingsystemsformalariatransmissionresearch
AT spitzenjeroen comparativeanalysisofglassandhemotekmembranefeedingsystemsformalariatransmissionresearch
AT bousemateun comparativeanalysisofglassandhemotekmembranefeedingsystemsformalariatransmissionresearch
AT miesenpascal comparativeanalysisofglassandhemotekmembranefeedingsystemsformalariatransmissionresearch

Ejemplares similares