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Modulation of deep neural circuits with sonogenetics
Noninvasive control of neuronal activity in the deep brain can be illuminating for probing brain function and treating dysfunctions. Here, we present a sonogenetic approach for controlling distinct mouse behavior with circuit specificity and subsecond temporal resolution. Targeted neurons in subcort...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235981/ https://www.ncbi.nlm.nih.gov/pubmed/37216521 http://dx.doi.org/10.1073/pnas.2220575120 |
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author | Xian, Quanxiang Qiu, Zhihai Murugappan, Suresh Kala, Shashwati Wong, Kin Fung Li, Danni Li, Guofeng Jiang, Yizhou Wu, Yong Su, Min Hou, Xuandi Zhu, Jiejun Guo, Jinghui Qiu, Weibao Sun, Lei |
author_facet | Xian, Quanxiang Qiu, Zhihai Murugappan, Suresh Kala, Shashwati Wong, Kin Fung Li, Danni Li, Guofeng Jiang, Yizhou Wu, Yong Su, Min Hou, Xuandi Zhu, Jiejun Guo, Jinghui Qiu, Weibao Sun, Lei |
author_sort | Xian, Quanxiang |
collection | PubMed |
description | Noninvasive control of neuronal activity in the deep brain can be illuminating for probing brain function and treating dysfunctions. Here, we present a sonogenetic approach for controlling distinct mouse behavior with circuit specificity and subsecond temporal resolution. Targeted neurons in subcortical regions were made to express a mutant large conductance mechanosensitive ion channel (MscL-G22S), enabling ultrasound to trigger activity in MscL-expressing neurons in the dorsal striatum and increase locomotion in freely moving mice. Ultrasound stimulation of MscL-expressing neurons in the ventral tegmental area could activate the mesolimbic pathway to trigger dopamine release in the nucleus accumbens and modulate appetitive conditioning. Moreover, sonogenetic stimulation of the subthalamic nuclei of Parkinson’s disease model mice improved their motor coordination and mobile time. Neuronal responses to ultrasound pulse trains were rapid, reversible, and repeatable. We also confirmed that the MscL-G22S mutant is more effective to sensitize neurons to ultrasound compared to the wild-type MscL. Altogether, we lay out a sonogenetic approach which can selectively manipulate targeted cells to activate defined neural pathways, affect specific behaviors, and relieve symptoms of neurodegenerative disease. |
format | Online Article Text |
id | pubmed-10235981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-102359812023-06-03 Modulation of deep neural circuits with sonogenetics Xian, Quanxiang Qiu, Zhihai Murugappan, Suresh Kala, Shashwati Wong, Kin Fung Li, Danni Li, Guofeng Jiang, Yizhou Wu, Yong Su, Min Hou, Xuandi Zhu, Jiejun Guo, Jinghui Qiu, Weibao Sun, Lei Proc Natl Acad Sci U S A Biological Sciences Noninvasive control of neuronal activity in the deep brain can be illuminating for probing brain function and treating dysfunctions. Here, we present a sonogenetic approach for controlling distinct mouse behavior with circuit specificity and subsecond temporal resolution. Targeted neurons in subcortical regions were made to express a mutant large conductance mechanosensitive ion channel (MscL-G22S), enabling ultrasound to trigger activity in MscL-expressing neurons in the dorsal striatum and increase locomotion in freely moving mice. Ultrasound stimulation of MscL-expressing neurons in the ventral tegmental area could activate the mesolimbic pathway to trigger dopamine release in the nucleus accumbens and modulate appetitive conditioning. Moreover, sonogenetic stimulation of the subthalamic nuclei of Parkinson’s disease model mice improved their motor coordination and mobile time. Neuronal responses to ultrasound pulse trains were rapid, reversible, and repeatable. We also confirmed that the MscL-G22S mutant is more effective to sensitize neurons to ultrasound compared to the wild-type MscL. Altogether, we lay out a sonogenetic approach which can selectively manipulate targeted cells to activate defined neural pathways, affect specific behaviors, and relieve symptoms of neurodegenerative disease. National Academy of Sciences 2023-05-22 2023-05-30 /pmc/articles/PMC10235981/ /pubmed/37216521 http://dx.doi.org/10.1073/pnas.2220575120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Xian, Quanxiang Qiu, Zhihai Murugappan, Suresh Kala, Shashwati Wong, Kin Fung Li, Danni Li, Guofeng Jiang, Yizhou Wu, Yong Su, Min Hou, Xuandi Zhu, Jiejun Guo, Jinghui Qiu, Weibao Sun, Lei Modulation of deep neural circuits with sonogenetics |
title | Modulation of deep neural circuits with sonogenetics |
title_full | Modulation of deep neural circuits with sonogenetics |
title_fullStr | Modulation of deep neural circuits with sonogenetics |
title_full_unstemmed | Modulation of deep neural circuits with sonogenetics |
title_short | Modulation of deep neural circuits with sonogenetics |
title_sort | modulation of deep neural circuits with sonogenetics |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235981/ https://www.ncbi.nlm.nih.gov/pubmed/37216521 http://dx.doi.org/10.1073/pnas.2220575120 |
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