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A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh
The ‘Honeycrisp’ apple, an economically important cultivar and breeding parent, is prone to soft scald and soggy breakdown postharvest physiological disorders. Phenotypic evaluation of soft scald is time consuming and costly, making it an excellent target for DNA-informed breeding. The objective of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236029/ https://www.ncbi.nlm.nih.gov/pubmed/37309399 http://dx.doi.org/10.1007/s11032-021-01245-w |
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author | Miller, Baylee A. Tillman, John R. Howard, Nicholas P. Kostick, Sarah A. Evans, Kate M. Luby, James J. |
author_facet | Miller, Baylee A. Tillman, John R. Howard, Nicholas P. Kostick, Sarah A. Evans, Kate M. Luby, James J. |
author_sort | Miller, Baylee A. |
collection | PubMed |
description | The ‘Honeycrisp’ apple, an economically important cultivar and breeding parent, is prone to soft scald and soggy breakdown postharvest physiological disorders. Phenotypic evaluation of soft scald is time consuming and costly, making it an excellent target for DNA-informed breeding. The objective of this study was to develop a DNA test for a soft scald and soggy breakdown quantitative trait locus (QTL) on linkage group two (LG2) that was characterized in a previous study. ‘Honeycrisp’ is homozygous for the undesirable high disorder incidence haplotype (HDI) at this QTL. In this study, sixteen single nucleotide polymorphism markers were evaluated for their associations with the HDI haplotype in a set of 132 unique cultivars and important breeding parents. A DNA test was successfully developed utilizing KASP™ (Kompetitive Allele Specific PCR) chemistry to identify the number of HDI haplotypes in individuals. This test had a 100% accuracy for detecting homozygous unfavorable HDI individuals and has an expected 88% accuracy over all three haplotype copy groups across the evaluated germplasm. This DNA test is a promising tool for minimizing the chances of selecting individuals that exhibit high incidence of soft scald postharvest disorder in ‘Honeycrisp’-related germplasm. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-021-01245-w. |
format | Online Article Text |
id | pubmed-10236029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-102360292023-06-12 A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh Miller, Baylee A. Tillman, John R. Howard, Nicholas P. Kostick, Sarah A. Evans, Kate M. Luby, James J. Mol Breed Article The ‘Honeycrisp’ apple, an economically important cultivar and breeding parent, is prone to soft scald and soggy breakdown postharvest physiological disorders. Phenotypic evaluation of soft scald is time consuming and costly, making it an excellent target for DNA-informed breeding. The objective of this study was to develop a DNA test for a soft scald and soggy breakdown quantitative trait locus (QTL) on linkage group two (LG2) that was characterized in a previous study. ‘Honeycrisp’ is homozygous for the undesirable high disorder incidence haplotype (HDI) at this QTL. In this study, sixteen single nucleotide polymorphism markers were evaluated for their associations with the HDI haplotype in a set of 132 unique cultivars and important breeding parents. A DNA test was successfully developed utilizing KASP™ (Kompetitive Allele Specific PCR) chemistry to identify the number of HDI haplotypes in individuals. This test had a 100% accuracy for detecting homozygous unfavorable HDI individuals and has an expected 88% accuracy over all three haplotype copy groups across the evaluated germplasm. This DNA test is a promising tool for minimizing the chances of selecting individuals that exhibit high incidence of soft scald postharvest disorder in ‘Honeycrisp’-related germplasm. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-021-01245-w. Springer Netherlands 2021-09-11 /pmc/articles/PMC10236029/ /pubmed/37309399 http://dx.doi.org/10.1007/s11032-021-01245-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Miller, Baylee A. Tillman, John R. Howard, Nicholas P. Kostick, Sarah A. Evans, Kate M. Luby, James J. A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh |
title | A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh |
title_full | A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh |
title_fullStr | A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh |
title_full_unstemmed | A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh |
title_short | A DNA test for high incidence of soft scald and soggy breakdown postharvest disorders in Malus domestica Borkh |
title_sort | dna test for high incidence of soft scald and soggy breakdown postharvest disorders in malus domestica borkh |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236029/ https://www.ncbi.nlm.nih.gov/pubmed/37309399 http://dx.doi.org/10.1007/s11032-021-01245-w |
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