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Chromosome karyotype and stability of new synthetic hexaploid wheat

Synthetic hexaploid wheat offers breeders ready access to potentially novel genetic variation in wild ancestral species. In this study, we crossed MY3478 (2n = 4x = 28, AABB) as the maternal parent with the stripe rust–resistant SY41 (2n = 2x = 14, DD) as the paternal parent to construct the new hex...

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Autores principales: Wang, Yajuan, Wang, Siwen, Jia, Xiujuan, Tian, Zengrong, Wang, Yongfu, Wang, Changyou, Zhang, Hong, Liu, Xinlun, Zhao, Jixin, Deng, Pingchuan, Ji, Wanquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236053/
https://www.ncbi.nlm.nih.gov/pubmed/37309315
http://dx.doi.org/10.1007/s11032-021-01253-w
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author Wang, Yajuan
Wang, Siwen
Jia, Xiujuan
Tian, Zengrong
Wang, Yongfu
Wang, Changyou
Zhang, Hong
Liu, Xinlun
Zhao, Jixin
Deng, Pingchuan
Ji, Wanquan
author_facet Wang, Yajuan
Wang, Siwen
Jia, Xiujuan
Tian, Zengrong
Wang, Yongfu
Wang, Changyou
Zhang, Hong
Liu, Xinlun
Zhao, Jixin
Deng, Pingchuan
Ji, Wanquan
author_sort Wang, Yajuan
collection PubMed
description Synthetic hexaploid wheat offers breeders ready access to potentially novel genetic variation in wild ancestral species. In this study, we crossed MY3478 (2n = 4x = 28, AABB) as the maternal parent with the stripe rust–resistant SY41 (2n = 2x = 14, DD) as the paternal parent to construct the new hexaploid wheat line NA0928 through natural allopolyploidization. Agronomic traits and the cytology of the S(8)–S(9) generations of NA0928 were analyzed. Abundant variation in agronomic traits was observed among each strain of NA0928 in the S(8) generation. Agronomic traits were superior in strains resistant to stripe rust compared with those of highly susceptible strains. The rank order of the coefficients of variation were tiller number (55.3%) > spike length (15.3%) > number of spikelets (13.9%) > plant height (8.7). Number of tillers and spike length are important traits in wheat breeding to improve yield. Cytological observation and fluorescence in situ hybridization showed that the chromosome number and configuration showed rich variation among NA0928 strains in the S(9) generation. Chromosome number ranged from 36 to 44. Variation in chromosome karyotype was detected in the A and B subgenomes. Meiotic chromosome behavior in pollen mother cells and multicolor genomic in situ hybridization revealed that two new synthetic hexaploid wheat strains showed genetic stability; one strain was resistant to stripe rust and developed multiple tillers, and the other strain was susceptible to stripe rust, but both showed improved thousand-kernel weight (TKW) weight and produced multiple tillers. The two strains will be valuable germplasm resources for use in wheat breeding.
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spelling pubmed-102360532023-06-12 Chromosome karyotype and stability of new synthetic hexaploid wheat Wang, Yajuan Wang, Siwen Jia, Xiujuan Tian, Zengrong Wang, Yongfu Wang, Changyou Zhang, Hong Liu, Xinlun Zhao, Jixin Deng, Pingchuan Ji, Wanquan Mol Breed Article Synthetic hexaploid wheat offers breeders ready access to potentially novel genetic variation in wild ancestral species. In this study, we crossed MY3478 (2n = 4x = 28, AABB) as the maternal parent with the stripe rust–resistant SY41 (2n = 2x = 14, DD) as the paternal parent to construct the new hexaploid wheat line NA0928 through natural allopolyploidization. Agronomic traits and the cytology of the S(8)–S(9) generations of NA0928 were analyzed. Abundant variation in agronomic traits was observed among each strain of NA0928 in the S(8) generation. Agronomic traits were superior in strains resistant to stripe rust compared with those of highly susceptible strains. The rank order of the coefficients of variation were tiller number (55.3%) > spike length (15.3%) > number of spikelets (13.9%) > plant height (8.7). Number of tillers and spike length are important traits in wheat breeding to improve yield. Cytological observation and fluorescence in situ hybridization showed that the chromosome number and configuration showed rich variation among NA0928 strains in the S(9) generation. Chromosome number ranged from 36 to 44. Variation in chromosome karyotype was detected in the A and B subgenomes. Meiotic chromosome behavior in pollen mother cells and multicolor genomic in situ hybridization revealed that two new synthetic hexaploid wheat strains showed genetic stability; one strain was resistant to stripe rust and developed multiple tillers, and the other strain was susceptible to stripe rust, but both showed improved thousand-kernel weight (TKW) weight and produced multiple tillers. The two strains will be valuable germplasm resources for use in wheat breeding. Springer Netherlands 2021-09-24 /pmc/articles/PMC10236053/ /pubmed/37309315 http://dx.doi.org/10.1007/s11032-021-01253-w Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2021 https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Yajuan
Wang, Siwen
Jia, Xiujuan
Tian, Zengrong
Wang, Yongfu
Wang, Changyou
Zhang, Hong
Liu, Xinlun
Zhao, Jixin
Deng, Pingchuan
Ji, Wanquan
Chromosome karyotype and stability of new synthetic hexaploid wheat
title Chromosome karyotype and stability of new synthetic hexaploid wheat
title_full Chromosome karyotype and stability of new synthetic hexaploid wheat
title_fullStr Chromosome karyotype and stability of new synthetic hexaploid wheat
title_full_unstemmed Chromosome karyotype and stability of new synthetic hexaploid wheat
title_short Chromosome karyotype and stability of new synthetic hexaploid wheat
title_sort chromosome karyotype and stability of new synthetic hexaploid wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236053/
https://www.ncbi.nlm.nih.gov/pubmed/37309315
http://dx.doi.org/10.1007/s11032-021-01253-w
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