Cargando…
CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. It has been reported that cysteine rich protein 1 (CRP-1) is dysregulated in several types of human cancer; however, its role in HCC is poorly understood. Therefore, the current study aimed to investigate the role of CR...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236095/ https://www.ncbi.nlm.nih.gov/pubmed/37273753 http://dx.doi.org/10.3892/etm.2023.12013 |
_version_ | 1785052840374304768 |
---|---|
author | Lei, Shixiong Du, Xilin Tan, Kai He, Xiaojun Zhu, Yejing Zhao, Shoujie Yang, Zhenyu Dou, Gang |
author_facet | Lei, Shixiong Du, Xilin Tan, Kai He, Xiaojun Zhu, Yejing Zhao, Shoujie Yang, Zhenyu Dou, Gang |
author_sort | Lei, Shixiong |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. It has been reported that cysteine rich protein 1 (CRP-1) is dysregulated in several types of human cancer; however, its role in HCC is poorly understood. Therefore, the current study aimed to investigate the role of CRP-1 in HCC. Western blotting and reverse transcription-quantitative PCR results showed that CRP-1 was upregulated in HCC cell lines. Furthermore, for in vitro experiments, CRP-1 was knocked down and overexpressed in the HCC cell lines Hep 3B2.1-7 and BEL-7405, respectively. c-Myc and proliferating cell nuclear antigen upregulation, and cleaved caspase 3 and poly(ADP-ribose) polymerase downregulation suggested that CRP-1 silencing could inhibit the proliferation and colony-forming ability of HCC cells, and induce apoptosis. In addition, CRP-1 overexpression promoted the malignant behavior of HCC cells and induced epithelial-mesenchymal transition (EMT), as verified by E-cadherin downregulation, and N-cadherin and vimentin upregulation. Additionally, CRP-1 overexpression promoted the nuclear translocation of β-catenin, and activated the expression of cyclin D1 and matrix metalloproteinase-7. Furthermore, inhibition of Wnt/β-catenin signaling, following cell treatment with XAV-939, an inhibitor of the Wnt/β-catenin signaling pathway, abrogated the effects of CRP-1 on enhancing the proliferation and migration of HCC cells. These findings indicated that the regulatory effect of CRP-1 on HCC cells could be mediated by the Wnt/β-catenin signaling pathway. Overall, CRP-1 could promote the proliferation and migration of HCC cell lines, partially via promoting EMT and activating the Wnt/β-catenin signaling pathway. |
format | Online Article Text |
id | pubmed-10236095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-102360952023-06-03 CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling Lei, Shixiong Du, Xilin Tan, Kai He, Xiaojun Zhu, Yejing Zhao, Shoujie Yang, Zhenyu Dou, Gang Exp Ther Med Articles Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. It has been reported that cysteine rich protein 1 (CRP-1) is dysregulated in several types of human cancer; however, its role in HCC is poorly understood. Therefore, the current study aimed to investigate the role of CRP-1 in HCC. Western blotting and reverse transcription-quantitative PCR results showed that CRP-1 was upregulated in HCC cell lines. Furthermore, for in vitro experiments, CRP-1 was knocked down and overexpressed in the HCC cell lines Hep 3B2.1-7 and BEL-7405, respectively. c-Myc and proliferating cell nuclear antigen upregulation, and cleaved caspase 3 and poly(ADP-ribose) polymerase downregulation suggested that CRP-1 silencing could inhibit the proliferation and colony-forming ability of HCC cells, and induce apoptosis. In addition, CRP-1 overexpression promoted the malignant behavior of HCC cells and induced epithelial-mesenchymal transition (EMT), as verified by E-cadherin downregulation, and N-cadherin and vimentin upregulation. Additionally, CRP-1 overexpression promoted the nuclear translocation of β-catenin, and activated the expression of cyclin D1 and matrix metalloproteinase-7. Furthermore, inhibition of Wnt/β-catenin signaling, following cell treatment with XAV-939, an inhibitor of the Wnt/β-catenin signaling pathway, abrogated the effects of CRP-1 on enhancing the proliferation and migration of HCC cells. These findings indicated that the regulatory effect of CRP-1 on HCC cells could be mediated by the Wnt/β-catenin signaling pathway. Overall, CRP-1 could promote the proliferation and migration of HCC cell lines, partially via promoting EMT and activating the Wnt/β-catenin signaling pathway. D.A. Spandidos 2023-05-12 /pmc/articles/PMC10236095/ /pubmed/37273753 http://dx.doi.org/10.3892/etm.2023.12013 Text en Copyright: © Lei et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Lei, Shixiong Du, Xilin Tan, Kai He, Xiaojun Zhu, Yejing Zhao, Shoujie Yang, Zhenyu Dou, Gang CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling |
title | CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling |
title_full | CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling |
title_fullStr | CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling |
title_full_unstemmed | CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling |
title_short | CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling |
title_sort | crp‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and wnt/β‑catenin signaling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236095/ https://www.ncbi.nlm.nih.gov/pubmed/37273753 http://dx.doi.org/10.3892/etm.2023.12013 |
work_keys_str_mv | AT leishixiong crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT duxilin crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT tankai crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT hexiaojun crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT zhuyejing crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT zhaoshoujie crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT yangzhenyu crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling AT dougang crp1promotesthemalignantbehaviorofhepatocellularcarcinomacellsviaactivatingepithelialmesenchymaltransitionandwntbcateninsignaling |