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Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food

We investigated the probiotic characteristics and anti-obesity effect of Lactiplantibacillus plantarum MGEL20154, a strain that possesses excellent intestinal adhesion and viability. The in vitro properties, e.g., gastrointestinal (GI) resistance, adhesion, and enzyme activity, demonstrated that MGE...

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Autores principales: Park, So Young, Choi, Jin Won, Oh, Dong Nyoung, Lee, Eun Ji, Kim, Dong Pil, Yoon, Sun Jay, Jang, Won Je, Han, Sang Jun, Lee, Seungjun, Lee, Jong Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236178/
https://www.ncbi.nlm.nih.gov/pubmed/36864459
http://dx.doi.org/10.4014/jmb.2212.12005
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author Park, So Young
Choi, Jin Won
Oh, Dong Nyoung
Lee, Eun Ji
Kim, Dong Pil
Yoon, Sun Jay
Jang, Won Je
Han, Sang Jun
Lee, Seungjun
Lee, Jong Min
author_facet Park, So Young
Choi, Jin Won
Oh, Dong Nyoung
Lee, Eun Ji
Kim, Dong Pil
Yoon, Sun Jay
Jang, Won Je
Han, Sang Jun
Lee, Seungjun
Lee, Jong Min
author_sort Park, So Young
collection PubMed
description We investigated the probiotic characteristics and anti-obesity effect of Lactiplantibacillus plantarum MGEL20154, a strain that possesses excellent intestinal adhesion and viability. The in vitro properties, e.g., gastrointestinal (GI) resistance, adhesion, and enzyme activity, demonstrated that MGEL20154 is a potential probiotic candidate. Oral administration of MGEL20154 to diet-induced obese C57BL/6J mice for 8 weeks resulted in a feed efficacy decrease by 44.7% compared to that of the high-fat diet (HFD) group. The reduction rate of weight gain was about 48.5% in the HFD+MGEL20154 group compared to that of the HFD group after 8 weeks, and the epididymal fat pad was also reduced in size by 25.2%. In addition, the upregulation of the zo-1, pparα, and erk2, and downregulation of the nf-κb and glut2 genes in Caco-2 cells by MGEL20154 were observed. Therefore, we propose that the anti-obesity effect of the strain is exerted by inhibiting carbohydrate absorption and regulating gene expression in the intestine.
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spelling pubmed-102361782023-06-03 Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food Park, So Young Choi, Jin Won Oh, Dong Nyoung Lee, Eun Ji Kim, Dong Pil Yoon, Sun Jay Jang, Won Je Han, Sang Jun Lee, Seungjun Lee, Jong Min J Microbiol Biotechnol Research article We investigated the probiotic characteristics and anti-obesity effect of Lactiplantibacillus plantarum MGEL20154, a strain that possesses excellent intestinal adhesion and viability. The in vitro properties, e.g., gastrointestinal (GI) resistance, adhesion, and enzyme activity, demonstrated that MGEL20154 is a potential probiotic candidate. Oral administration of MGEL20154 to diet-induced obese C57BL/6J mice for 8 weeks resulted in a feed efficacy decrease by 44.7% compared to that of the high-fat diet (HFD) group. The reduction rate of weight gain was about 48.5% in the HFD+MGEL20154 group compared to that of the HFD group after 8 weeks, and the epididymal fat pad was also reduced in size by 25.2%. In addition, the upregulation of the zo-1, pparα, and erk2, and downregulation of the nf-κb and glut2 genes in Caco-2 cells by MGEL20154 were observed. Therefore, we propose that the anti-obesity effect of the strain is exerted by inhibiting carbohydrate absorption and regulating gene expression in the intestine. The Korean Society for Microbiology and Biotechnology 2023-05-28 2023-02-06 /pmc/articles/PMC10236178/ /pubmed/36864459 http://dx.doi.org/10.4014/jmb.2212.12005 Text en Copyright © 2023 by the authors. Licensee KMB https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Research article
Park, So Young
Choi, Jin Won
Oh, Dong Nyoung
Lee, Eun Ji
Kim, Dong Pil
Yoon, Sun Jay
Jang, Won Je
Han, Sang Jun
Lee, Seungjun
Lee, Jong Min
Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food
title Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food
title_full Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food
title_fullStr Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food
title_full_unstemmed Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food
title_short Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food
title_sort anti-obesity potential through regulation of carbohydrate uptake and gene expression in intestinal epithelial cells by the probiotic lactiplantibacillus plantarum mgel20154 from fermented food
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236178/
https://www.ncbi.nlm.nih.gov/pubmed/36864459
http://dx.doi.org/10.4014/jmb.2212.12005
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