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3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency
Limbal epithelial stem cells (LESCs) are responsible for the maintenance and repair of the corneal surface. Injuries and diseases of the eye may result in a vision condition called limbal stem cell deficiency (LSCD). Without limbal stem cells, the cornea becomes opaque, vascularized, and inflamed. C...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Whioce Publishing Pte. Ltd.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236339/ https://www.ncbi.nlm.nih.gov/pubmed/37273996 http://dx.doi.org/10.18063/ijb.710 |
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author | Xue, Qian Ma, Liang Hu, Hanyi Li, Qi Wang, Weiwei Yang, Huayong Zhang, Bin |
author_facet | Xue, Qian Ma, Liang Hu, Hanyi Li, Qi Wang, Weiwei Yang, Huayong Zhang, Bin |
author_sort | Xue, Qian |
collection | PubMed |
description | Limbal epithelial stem cells (LESCs) are responsible for the maintenance and repair of the corneal surface. Injuries and diseases of the eye may result in a vision condition called limbal stem cell deficiency (LSCD). Without limbal stem cells, the cornea becomes opaque, vascularized, and inflamed. Cultured LESC therapy as a treatment method was first described in 1997, and LESCs cultured from either patients or donors have been used to treat LSCD successfully. However, the main source of cornea for LSCD treatment is from donors, which are too few to meet the demand (less than 1:70 of cases). The global shortage of donor cornea promotes the need for studies exploring corneal limbus alternatives. Many problems still remain unresolved, such as original geometry reconstruction, corneal epithelial regeneration, and ocular optical function restoration. 3D bioprinting has garnered tremendous attention in recent years, and significant advances have been made in fabricating cell–laden scaffolds. These advancements could lead to a promising treatment for LSCD. It is possible that alternative limbus stem cells can be constructed using 3D printing, which, in corneal limbus regeneration, enables personalized corneal implants and fabrication of single- or multilayer corneal limbus equivalents with corneal limbal stem cells. In this review, the progress, applications, and limitations of the most influential works regarding current treatments of LESC deficiency are discussed. The advantages of 3D bioprinting are illustrated, and some first promising steps toward the creation of a functional cornea limbus with 3D bioprinting are discussed. Finally, insights into the prospects and technical challenges facing the future research of 3D bioprinting of corneal limbus alternatives in vivo and in vitro are provided. |
format | Online Article Text |
id | pubmed-10236339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Whioce Publishing Pte. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102363392023-06-03 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency Xue, Qian Ma, Liang Hu, Hanyi Li, Qi Wang, Weiwei Yang, Huayong Zhang, Bin Int J Bioprint Research Article Limbal epithelial stem cells (LESCs) are responsible for the maintenance and repair of the corneal surface. Injuries and diseases of the eye may result in a vision condition called limbal stem cell deficiency (LSCD). Without limbal stem cells, the cornea becomes opaque, vascularized, and inflamed. Cultured LESC therapy as a treatment method was first described in 1997, and LESCs cultured from either patients or donors have been used to treat LSCD successfully. However, the main source of cornea for LSCD treatment is from donors, which are too few to meet the demand (less than 1:70 of cases). The global shortage of donor cornea promotes the need for studies exploring corneal limbus alternatives. Many problems still remain unresolved, such as original geometry reconstruction, corneal epithelial regeneration, and ocular optical function restoration. 3D bioprinting has garnered tremendous attention in recent years, and significant advances have been made in fabricating cell–laden scaffolds. These advancements could lead to a promising treatment for LSCD. It is possible that alternative limbus stem cells can be constructed using 3D printing, which, in corneal limbus regeneration, enables personalized corneal implants and fabrication of single- or multilayer corneal limbus equivalents with corneal limbal stem cells. In this review, the progress, applications, and limitations of the most influential works regarding current treatments of LESC deficiency are discussed. The advantages of 3D bioprinting are illustrated, and some first promising steps toward the creation of a functional cornea limbus with 3D bioprinting are discussed. Finally, insights into the prospects and technical challenges facing the future research of 3D bioprinting of corneal limbus alternatives in vivo and in vitro are provided. Whioce Publishing Pte. Ltd. 2023-03-15 /pmc/articles/PMC10236339/ /pubmed/37273996 http://dx.doi.org/10.18063/ijb.710 Text en Copyright:© 2023, Xue Q, Ma L, Hu H, et al https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xue, Qian Ma, Liang Hu, Hanyi Li, Qi Wang, Weiwei Yang, Huayong Zhang, Bin 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
title | 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
title_full | 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
title_fullStr | 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
title_full_unstemmed | 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
title_short | 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
title_sort | 3d bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236339/ https://www.ncbi.nlm.nih.gov/pubmed/37273996 http://dx.doi.org/10.18063/ijb.710 |
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