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Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7

INTRODUCTION: Bacteriophages infecting human pathogens have been considered potential biocontrol agents, and studying their genetic content is essential to their safe use in the food industry. Tequatrovirus ufvareg1 is a bacteriophage named UFV-AREG1, isolated from cowshed wastewater and previously...

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Autores principales: Lopez, Maryoris Elisa Soto, Gontijo, Marco Tulio Pardini, Cardoso, Rodrigo Rezende, Batalha, Laís Silva, Eller, Monique Renon, Bazzolli, Denise Mara Soares, Vidigal, Pedro Marcus Pereira, Mendonça, Regina Célia Santos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236363/
https://www.ncbi.nlm.nih.gov/pubmed/37274318
http://dx.doi.org/10.3389/fcimb.2023.1178248
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author Lopez, Maryoris Elisa Soto
Gontijo, Marco Tulio Pardini
Cardoso, Rodrigo Rezende
Batalha, Laís Silva
Eller, Monique Renon
Bazzolli, Denise Mara Soares
Vidigal, Pedro Marcus Pereira
Mendonça, Regina Célia Santos
author_facet Lopez, Maryoris Elisa Soto
Gontijo, Marco Tulio Pardini
Cardoso, Rodrigo Rezende
Batalha, Laís Silva
Eller, Monique Renon
Bazzolli, Denise Mara Soares
Vidigal, Pedro Marcus Pereira
Mendonça, Regina Célia Santos
author_sort Lopez, Maryoris Elisa Soto
collection PubMed
description INTRODUCTION: Bacteriophages infecting human pathogens have been considered potential biocontrol agents, and studying their genetic content is essential to their safe use in the food industry. Tequatrovirus ufvareg1 is a bacteriophage named UFV-AREG1, isolated from cowshed wastewater and previously tested for its ability to inhibit Escherichia coli O157:H7. METHODS: T. ufvareg1 was previously isolated using E. coli O157:H7 (ATCC 43895) as a bacterial host. The same strain was used for bacteriophage propagation and the one-step growth curve. The genome of the T. ufvareg1 was sequenced using 305 Illumina HiSeq, and the genome comparison was calculated by VIRIDIC and VIPTree. RESULTS: Here, we characterize its genome and compare it to other Tequatrovirus. T. ufvareg1 virions have an icosahedral head (114 x 86 nm) and a contracted tail (117 x 23 nm), with a latent period of 25 min, and an average burst size was 18 phage particles per infected E. coli cell. The genome of the bacteriophage T. ufvareg1 contains 268 coding DNA sequences (CDS) and ten tRNA genes distributed in both negative and positive strains. T. ufvareg1 genome also contains 40 promoters on its regulatory regions and two rho-independent terminators. T. ufvareg1 shares an average intergenomic similarity (VIRIDC) of 88.77% and an average genomic similarity score (VipTree) of 88.91% with eight four reference genomes for Tequatrovirus available in the NCBI RefSeq database. The pan-genomic analysis confirmed the high conservation of Tequatrovirus genomes. Among all CDS annotated in the T. ufvareg1 genome, there are 123 core genes, 38 softcore genes, 94 shell genes, and 13 cloud genes. None of 268 CDS was classified as being exclusive of T. ufvareg1. CONCLUSION: The results in this paper, combined with other previously published findings, indicate that T. ufvareg1 bacteriophage is a potential candidate for food protection against E. coli O157:H7 in foods.
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spelling pubmed-102363632023-06-03 Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7 Lopez, Maryoris Elisa Soto Gontijo, Marco Tulio Pardini Cardoso, Rodrigo Rezende Batalha, Laís Silva Eller, Monique Renon Bazzolli, Denise Mara Soares Vidigal, Pedro Marcus Pereira Mendonça, Regina Célia Santos Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: Bacteriophages infecting human pathogens have been considered potential biocontrol agents, and studying their genetic content is essential to their safe use in the food industry. Tequatrovirus ufvareg1 is a bacteriophage named UFV-AREG1, isolated from cowshed wastewater and previously tested for its ability to inhibit Escherichia coli O157:H7. METHODS: T. ufvareg1 was previously isolated using E. coli O157:H7 (ATCC 43895) as a bacterial host. The same strain was used for bacteriophage propagation and the one-step growth curve. The genome of the T. ufvareg1 was sequenced using 305 Illumina HiSeq, and the genome comparison was calculated by VIRIDIC and VIPTree. RESULTS: Here, we characterize its genome and compare it to other Tequatrovirus. T. ufvareg1 virions have an icosahedral head (114 x 86 nm) and a contracted tail (117 x 23 nm), with a latent period of 25 min, and an average burst size was 18 phage particles per infected E. coli cell. The genome of the bacteriophage T. ufvareg1 contains 268 coding DNA sequences (CDS) and ten tRNA genes distributed in both negative and positive strains. T. ufvareg1 genome also contains 40 promoters on its regulatory regions and two rho-independent terminators. T. ufvareg1 shares an average intergenomic similarity (VIRIDC) of 88.77% and an average genomic similarity score (VipTree) of 88.91% with eight four reference genomes for Tequatrovirus available in the NCBI RefSeq database. The pan-genomic analysis confirmed the high conservation of Tequatrovirus genomes. Among all CDS annotated in the T. ufvareg1 genome, there are 123 core genes, 38 softcore genes, 94 shell genes, and 13 cloud genes. None of 268 CDS was classified as being exclusive of T. ufvareg1. CONCLUSION: The results in this paper, combined with other previously published findings, indicate that T. ufvareg1 bacteriophage is a potential candidate for food protection against E. coli O157:H7 in foods. Frontiers Media S.A. 2023-05-16 /pmc/articles/PMC10236363/ /pubmed/37274318 http://dx.doi.org/10.3389/fcimb.2023.1178248 Text en Copyright © 2023 Lopez, Gontijo, Cardoso, Batalha, Eller, Bazzolli, Vidigal and Mendonça https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Lopez, Maryoris Elisa Soto
Gontijo, Marco Tulio Pardini
Cardoso, Rodrigo Rezende
Batalha, Laís Silva
Eller, Monique Renon
Bazzolli, Denise Mara Soares
Vidigal, Pedro Marcus Pereira
Mendonça, Regina Célia Santos
Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
title Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
title_full Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
title_fullStr Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
title_full_unstemmed Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
title_short Complete genome analysis of Tequatrovirus ufvareg1, a Tequatrovirus species inhibiting Escherichia coli O157:H7
title_sort complete genome analysis of tequatrovirus ufvareg1, a tequatrovirus species inhibiting escherichia coli o157:h7
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236363/
https://www.ncbi.nlm.nih.gov/pubmed/37274318
http://dx.doi.org/10.3389/fcimb.2023.1178248
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