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IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis

Primary infection during pregnancy by the protozoan Toxoplasma gondii can be worrisome because transmission to the fetus may lead to congenital toxoplasmosis (CT). Neonatal diagnosis is usually performed by serological profile comparison of the mother and newborn. As previously reported in 2012 by C...

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Autores principales: Peyclit, Lucie, Villard, Odile, Paris, Luc, Fricker-Hidalgo, Hélène, Houzé, Sandrine, Cimon, Bernard, Deleplancque, Anne-Sophie, Tournus, Céline, Pelloux, Hervé, Villena, Isabelle, Pomares, Christelle, L’Ollivier, Coralie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236937/
https://www.ncbi.nlm.nih.gov/pubmed/37265252
http://dx.doi.org/10.1051/parasite/2023020
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author Peyclit, Lucie
Villard, Odile
Paris, Luc
Fricker-Hidalgo, Hélène
Houzé, Sandrine
Cimon, Bernard
Deleplancque, Anne-Sophie
Tournus, Céline
Pelloux, Hervé
Villena, Isabelle
Pomares, Christelle
L’Ollivier, Coralie
author_facet Peyclit, Lucie
Villard, Odile
Paris, Luc
Fricker-Hidalgo, Hélène
Houzé, Sandrine
Cimon, Bernard
Deleplancque, Anne-Sophie
Tournus, Céline
Pelloux, Hervé
Villena, Isabelle
Pomares, Christelle
L’Ollivier, Coralie
author_sort Peyclit, Lucie
collection PubMed
description Primary infection during pregnancy by the protozoan Toxoplasma gondii can be worrisome because transmission to the fetus may lead to congenital toxoplasmosis (CT). Neonatal diagnosis is usually performed by serological profile comparison of the mother and newborn. As previously reported in 2012 by C. L’Ollivier et al., three IgM bands at 75, 90 and 100 kDa called the “IgM triplet” has caught our attention and seems to be pathognomonic of CT. This retrospective multicenter study involved nine reference laboratories included in the French National Reference Center for Toxoplasmosis network and concerned determining the specificity and sensitivity of this IgM triplet. On this basis, we were able to propose a new read of the comparison of IgG and IgM immunoblot profiles of mother and infant to increase the sensitivity of this diagnostic marker. The effect of the trimester of pregnancy at the time of infection, but also of maternal treatment with pyrimethamine/sulfadiazine/folinic acid on the presence of this IgM triplet in the infant, could be studied. The presence of the triplet appears pathognomonic for the diagnosis of CT, and it increased the sensitivity of the immunoblot assay from 55.04% to 72.48%. As a result, it would be wise to enhance conventional immunoblot reading by adding the presence of the three IgM bands in the infant pattern for neonatal diagnosis of CT.
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spelling pubmed-102369372023-06-03 IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis Peyclit, Lucie Villard, Odile Paris, Luc Fricker-Hidalgo, Hélène Houzé, Sandrine Cimon, Bernard Deleplancque, Anne-Sophie Tournus, Céline Pelloux, Hervé Villena, Isabelle Pomares, Christelle L’Ollivier, Coralie Parasite Research Article Primary infection during pregnancy by the protozoan Toxoplasma gondii can be worrisome because transmission to the fetus may lead to congenital toxoplasmosis (CT). Neonatal diagnosis is usually performed by serological profile comparison of the mother and newborn. As previously reported in 2012 by C. L’Ollivier et al., three IgM bands at 75, 90 and 100 kDa called the “IgM triplet” has caught our attention and seems to be pathognomonic of CT. This retrospective multicenter study involved nine reference laboratories included in the French National Reference Center for Toxoplasmosis network and concerned determining the specificity and sensitivity of this IgM triplet. On this basis, we were able to propose a new read of the comparison of IgG and IgM immunoblot profiles of mother and infant to increase the sensitivity of this diagnostic marker. The effect of the trimester of pregnancy at the time of infection, but also of maternal treatment with pyrimethamine/sulfadiazine/folinic acid on the presence of this IgM triplet in the infant, could be studied. The presence of the triplet appears pathognomonic for the diagnosis of CT, and it increased the sensitivity of the immunoblot assay from 55.04% to 72.48%. As a result, it would be wise to enhance conventional immunoblot reading by adding the presence of the three IgM bands in the infant pattern for neonatal diagnosis of CT. EDP Sciences 2023-06-02 /pmc/articles/PMC10236937/ /pubmed/37265252 http://dx.doi.org/10.1051/parasite/2023020 Text en © L. Peyclit et al., published by EDP Sciences, 2023 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Peyclit, Lucie
Villard, Odile
Paris, Luc
Fricker-Hidalgo, Hélène
Houzé, Sandrine
Cimon, Bernard
Deleplancque, Anne-Sophie
Tournus, Céline
Pelloux, Hervé
Villena, Isabelle
Pomares, Christelle
L’Ollivier, Coralie
IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
title IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
title_full IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
title_fullStr IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
title_full_unstemmed IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
title_short IgM triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
title_sort igm triplet in neonatal diagnosis by immunoblotting and its potential use as a diagnostic marker for congenital toxoplasmosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236937/
https://www.ncbi.nlm.nih.gov/pubmed/37265252
http://dx.doi.org/10.1051/parasite/2023020
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