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LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1

Geobacillus stearothermophilus is a highly thermophilic, spore-forming Gram-positive bacterium that causes flat sour spoilage in low-acid canned foods. To address this problem, we isolated G. stearothermophilus-infecting phage GR1 from the soil and characterized its endolysin LysGR1. Phage GR1 belon...

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Autores principales: Choi, Dahee, Kong, Minsuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237291/
https://www.ncbi.nlm.nih.gov/pubmed/37275144
http://dx.doi.org/10.3389/fmicb.2023.1178748
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author Choi, Dahee
Kong, Minsuk
author_facet Choi, Dahee
Kong, Minsuk
author_sort Choi, Dahee
collection PubMed
description Geobacillus stearothermophilus is a highly thermophilic, spore-forming Gram-positive bacterium that causes flat sour spoilage in low-acid canned foods. To address this problem, we isolated G. stearothermophilus-infecting phage GR1 from the soil and characterized its endolysin LysGR1. Phage GR1 belongs to the Siphoviridae family and possesses a genome of 79,387 DNA bps with 108 putative open reading frames. GR1 demonstrated a very low degree of homology to previously reported phages, indicating that it is novel. The endolysin of GR1 (LysGR1) contains an N-terminal amidase domain as an enzymatically active domain (EAD) and two C-terminal LysM domains as a cell wall binding domain (CBD). Although GR1 is specific to certain strains of G. stearothermophilus, LysGR1 showed a much broader lytic range, killing all the tested strains of G. stearothermophilus and several foodborne pathogens, such as Clostridium perfringens, Listeria monocytogenes, and Escherichia coli O157:H7. LysGR1_EAD, alone, also exhibits lytic activity against a wide range of bacteria, including Bacillus cereus, which is not terminated by a full-length endolysin. Both LysGR1 and its EAD effectively remove the G. stearothermophilus biofilms and are highly thermostable, retaining about 70% of their lytic activity after a 15-min incubation at 70°C. Considering the high thermal stability, broad lytic activity, and biofilm reduction efficacy of LysGR1 and its EAD, we hypothesize that these enzymes could act as promising biocontrol agents against G. stearothermophilus and as foodborne pathogens.
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spelling pubmed-102372912023-06-03 LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1 Choi, Dahee Kong, Minsuk Front Microbiol Microbiology Geobacillus stearothermophilus is a highly thermophilic, spore-forming Gram-positive bacterium that causes flat sour spoilage in low-acid canned foods. To address this problem, we isolated G. stearothermophilus-infecting phage GR1 from the soil and characterized its endolysin LysGR1. Phage GR1 belongs to the Siphoviridae family and possesses a genome of 79,387 DNA bps with 108 putative open reading frames. GR1 demonstrated a very low degree of homology to previously reported phages, indicating that it is novel. The endolysin of GR1 (LysGR1) contains an N-terminal amidase domain as an enzymatically active domain (EAD) and two C-terminal LysM domains as a cell wall binding domain (CBD). Although GR1 is specific to certain strains of G. stearothermophilus, LysGR1 showed a much broader lytic range, killing all the tested strains of G. stearothermophilus and several foodborne pathogens, such as Clostridium perfringens, Listeria monocytogenes, and Escherichia coli O157:H7. LysGR1_EAD, alone, also exhibits lytic activity against a wide range of bacteria, including Bacillus cereus, which is not terminated by a full-length endolysin. Both LysGR1 and its EAD effectively remove the G. stearothermophilus biofilms and are highly thermostable, retaining about 70% of their lytic activity after a 15-min incubation at 70°C. Considering the high thermal stability, broad lytic activity, and biofilm reduction efficacy of LysGR1 and its EAD, we hypothesize that these enzymes could act as promising biocontrol agents against G. stearothermophilus and as foodborne pathogens. Frontiers Media S.A. 2023-05-19 /pmc/articles/PMC10237291/ /pubmed/37275144 http://dx.doi.org/10.3389/fmicb.2023.1178748 Text en Copyright © 2023 Choi and Kong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Choi, Dahee
Kong, Minsuk
LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1
title LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1
title_full LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1
title_fullStr LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1
title_full_unstemmed LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1
title_short LysGR1, a novel thermostable endolysin from Geobacillus stearothermophilus bacteriophage GR1
title_sort lysgr1, a novel thermostable endolysin from geobacillus stearothermophilus bacteriophage gr1
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237291/
https://www.ncbi.nlm.nih.gov/pubmed/37275144
http://dx.doi.org/10.3389/fmicb.2023.1178748
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