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Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China

The emergence and spread of carbapenemase genes, colistin resistance genes mcr-1, and tigecycline resistance gene tet(X) represent a significant threat to clinical therapy and public health. In this study, we investigated the presence of carbapenemase genes, mcr-1, and tet(X) in 298 Escherichia coli...

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Autores principales: Sun, Lin, Sun, Guo-Zhuang, Jiang, Yue, Mei, Cai-Yue, Wang, Zhen-Yu, Wang, Han-Yun, Kong, Gui-Mei, Jiao, Xinan, Wang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237293/
https://www.ncbi.nlm.nih.gov/pubmed/37275145
http://dx.doi.org/10.3389/fmicb.2023.1181940
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author Sun, Lin
Sun, Guo-Zhuang
Jiang, Yue
Mei, Cai-Yue
Wang, Zhen-Yu
Wang, Han-Yun
Kong, Gui-Mei
Jiao, Xinan
Wang, Jing
author_facet Sun, Lin
Sun, Guo-Zhuang
Jiang, Yue
Mei, Cai-Yue
Wang, Zhen-Yu
Wang, Han-Yun
Kong, Gui-Mei
Jiao, Xinan
Wang, Jing
author_sort Sun, Lin
collection PubMed
description The emergence and spread of carbapenemase genes, colistin resistance genes mcr-1, and tigecycline resistance gene tet(X) represent a significant threat to clinical therapy and public health. In this study, we investigated the presence of carbapenemase genes, mcr-1, and tet(X) in 298 Escherichia coli strains obtained from a teaching hospital in China. In total, eight (2.68%), six (2.01%), and one (0.34%) E. coli isolates carried bla(NDM), mcr-1, and tet(X4), respectively. The bla(NDM) gene was located on IncX3 (n = 4), F2:A-:B- (n = 3), and F2:A1:B1 (n = 1) plasmids, with high similarity to multiple plasmids belonging to the same incompatibility type from Enterobacteriaceae. Six MCR-producing strains contained mcr-1-carrying IncI2 plasmids, organized similarly to other mcr-1-bearing IncI2 plasmids from animals in China. The bla(CTX−M−55/64/132/199) gene located within a typical transposition unit (ISEcp1-bla(CTX−M)-orf477Δ) was inserted near dnaJ to generate 5-bp direct repeats in four mcr-1-positive plasmids. The tet(X) and another four resistance genes [aadA2, tet(A), floR, and Δlnu(F)] were co-located on an IncX1 plasmid, highly similar to other tet(X4)-carrying IncX1 plasmids from Escherichia and Klebsiella of animal or food origin, except that the conjugative transfer region of IncX1 plasmids was absent in our plasmid. Although a low prevalence of bla(NDM), mcr-1, and tet(X) was observed in E. coli from patients in this study, their dissemination associated with some successful pandemic plasmids is of great concern. The continued surveillance of these crucial resistance genes in patients should be strengthened.
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spelling pubmed-102372932023-06-03 Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China Sun, Lin Sun, Guo-Zhuang Jiang, Yue Mei, Cai-Yue Wang, Zhen-Yu Wang, Han-Yun Kong, Gui-Mei Jiao, Xinan Wang, Jing Front Microbiol Microbiology The emergence and spread of carbapenemase genes, colistin resistance genes mcr-1, and tigecycline resistance gene tet(X) represent a significant threat to clinical therapy and public health. In this study, we investigated the presence of carbapenemase genes, mcr-1, and tet(X) in 298 Escherichia coli strains obtained from a teaching hospital in China. In total, eight (2.68%), six (2.01%), and one (0.34%) E. coli isolates carried bla(NDM), mcr-1, and tet(X4), respectively. The bla(NDM) gene was located on IncX3 (n = 4), F2:A-:B- (n = 3), and F2:A1:B1 (n = 1) plasmids, with high similarity to multiple plasmids belonging to the same incompatibility type from Enterobacteriaceae. Six MCR-producing strains contained mcr-1-carrying IncI2 plasmids, organized similarly to other mcr-1-bearing IncI2 plasmids from animals in China. The bla(CTX−M−55/64/132/199) gene located within a typical transposition unit (ISEcp1-bla(CTX−M)-orf477Δ) was inserted near dnaJ to generate 5-bp direct repeats in four mcr-1-positive plasmids. The tet(X) and another four resistance genes [aadA2, tet(A), floR, and Δlnu(F)] were co-located on an IncX1 plasmid, highly similar to other tet(X4)-carrying IncX1 plasmids from Escherichia and Klebsiella of animal or food origin, except that the conjugative transfer region of IncX1 plasmids was absent in our plasmid. Although a low prevalence of bla(NDM), mcr-1, and tet(X) was observed in E. coli from patients in this study, their dissemination associated with some successful pandemic plasmids is of great concern. The continued surveillance of these crucial resistance genes in patients should be strengthened. Frontiers Media S.A. 2023-05-19 /pmc/articles/PMC10237293/ /pubmed/37275145 http://dx.doi.org/10.3389/fmicb.2023.1181940 Text en Copyright © 2023 Sun, Sun, Jiang, Mei, Wang, Wang, Kong, Jiao and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sun, Lin
Sun, Guo-Zhuang
Jiang, Yue
Mei, Cai-Yue
Wang, Zhen-Yu
Wang, Han-Yun
Kong, Gui-Mei
Jiao, Xinan
Wang, Jing
Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China
title Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China
title_full Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China
title_fullStr Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China
title_full_unstemmed Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China
title_short Low prevalence of mobilized resistance genes bla(NDM), mcr-1, and tet(X4) in Escherichia coli from a hospital in China
title_sort low prevalence of mobilized resistance genes bla(ndm), mcr-1, and tet(x4) in escherichia coli from a hospital in china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237293/
https://www.ncbi.nlm.nih.gov/pubmed/37275145
http://dx.doi.org/10.3389/fmicb.2023.1181940
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