Cargando…

Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress

Helianthus tuberosus L. (Jerusalem artichoke) produce inulin, a type of fructan, which possesses several biotechnology applications, e.g., sugar syrup, prebiotics, fiber in diabetic food, enabling blood sugar and cholesterol reduction. Drought reduces inulin accumulation in the tubers of Jerusalem a...

Descripción completa

Detalles Bibliográficos
Autores principales: Boonmahome, Patcha, Namwongsa, Junthima, Vorasoot, Nimitr, Jogloy, Sanun, Riddech, Nuntavan, Boonlue, Sophon, Mongkolthanaruk, Wiyada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237377/
https://www.ncbi.nlm.nih.gov/pubmed/37267258
http://dx.doi.org/10.1371/journal.pone.0286625
_version_ 1785053143965368320
author Boonmahome, Patcha
Namwongsa, Junthima
Vorasoot, Nimitr
Jogloy, Sanun
Riddech, Nuntavan
Boonlue, Sophon
Mongkolthanaruk, Wiyada
author_facet Boonmahome, Patcha
Namwongsa, Junthima
Vorasoot, Nimitr
Jogloy, Sanun
Riddech, Nuntavan
Boonlue, Sophon
Mongkolthanaruk, Wiyada
author_sort Boonmahome, Patcha
collection PubMed
description Helianthus tuberosus L. (Jerusalem artichoke) produce inulin, a type of fructan, which possesses several biotechnology applications, e.g., sugar syrup, prebiotics, fiber in diabetic food, enabling blood sugar and cholesterol reduction. Drought reduces inulin accumulation in the tubers of Jerusalem artichoke as the plants protect themselves from this stress by induction of stress gene responses, effecting growth reduction. Endophytic bacteria are promising candidates to promote plant growth and yield particularly under abiotic stress. Therefore, three endophytic bacteria with plant growth promoting properties were examined for their ability to improve Jerusalem artichoke growth and yield under both well-watered and drought conditions when inoculated individually or in combinations in pot experiments with 2 factorial random complete block design. The interactions of the endophytic bacteria and plant host determined the differential gene expression in response to drought as revealed by quantitative polymerase chain reaction. Single inoculum of the endophytic bacteria increased the height, weight, root traits, and harvest index of Jerusalem artichoke compared to co-inocula under both well-watered and drought conditions. However, the co-inocula of Rossellomorea aquimaris strain 3.13 and Bacillus velezensis strain 5.18 proved to be a synergistic combination leading to high inulin accumulation; while the co-inocula of B. velezensis strain 5.18 and Micrococcus luteus strain 4.43 were not beneficial when used in combination. The genes, dehydrin like protein and ethylene responsive element binding factor, were upregulated in the plants inoculated with single inoculum and co-inocula of all endophytic bacteria during drought stress. Moreover, the gene expression of indole-3-acetic acid (IAA) amido synthetase were up-regulated in Jerusalem artichoke inoculated with M. luteus strain 4.43 during drought stress. The fructan:fructan 1-fructosyltransferase (1-FFT) was also stimulated by the endophytic bacteria particularly in drought condition; the results of this study could explain the relationship between endophytic bacteria and plant host for growth and yield promotion under well-watered and drought conditions.
format Online
Article
Text
id pubmed-10237377
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-102373772023-06-03 Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress Boonmahome, Patcha Namwongsa, Junthima Vorasoot, Nimitr Jogloy, Sanun Riddech, Nuntavan Boonlue, Sophon Mongkolthanaruk, Wiyada PLoS One Research Article Helianthus tuberosus L. (Jerusalem artichoke) produce inulin, a type of fructan, which possesses several biotechnology applications, e.g., sugar syrup, prebiotics, fiber in diabetic food, enabling blood sugar and cholesterol reduction. Drought reduces inulin accumulation in the tubers of Jerusalem artichoke as the plants protect themselves from this stress by induction of stress gene responses, effecting growth reduction. Endophytic bacteria are promising candidates to promote plant growth and yield particularly under abiotic stress. Therefore, three endophytic bacteria with plant growth promoting properties were examined for their ability to improve Jerusalem artichoke growth and yield under both well-watered and drought conditions when inoculated individually or in combinations in pot experiments with 2 factorial random complete block design. The interactions of the endophytic bacteria and plant host determined the differential gene expression in response to drought as revealed by quantitative polymerase chain reaction. Single inoculum of the endophytic bacteria increased the height, weight, root traits, and harvest index of Jerusalem artichoke compared to co-inocula under both well-watered and drought conditions. However, the co-inocula of Rossellomorea aquimaris strain 3.13 and Bacillus velezensis strain 5.18 proved to be a synergistic combination leading to high inulin accumulation; while the co-inocula of B. velezensis strain 5.18 and Micrococcus luteus strain 4.43 were not beneficial when used in combination. The genes, dehydrin like protein and ethylene responsive element binding factor, were upregulated in the plants inoculated with single inoculum and co-inocula of all endophytic bacteria during drought stress. Moreover, the gene expression of indole-3-acetic acid (IAA) amido synthetase were up-regulated in Jerusalem artichoke inoculated with M. luteus strain 4.43 during drought stress. The fructan:fructan 1-fructosyltransferase (1-FFT) was also stimulated by the endophytic bacteria particularly in drought condition; the results of this study could explain the relationship between endophytic bacteria and plant host for growth and yield promotion under well-watered and drought conditions. Public Library of Science 2023-06-02 /pmc/articles/PMC10237377/ /pubmed/37267258 http://dx.doi.org/10.1371/journal.pone.0286625 Text en © 2023 Boonmahome et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Boonmahome, Patcha
Namwongsa, Junthima
Vorasoot, Nimitr
Jogloy, Sanun
Riddech, Nuntavan
Boonlue, Sophon
Mongkolthanaruk, Wiyada
Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress
title Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress
title_full Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress
title_fullStr Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress
title_full_unstemmed Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress
title_short Single and co-inoculum of endophytic bacteria promote growth and yield of Jerusalem artichoke through upregulation of plant genes under drought stress
title_sort single and co-inoculum of endophytic bacteria promote growth and yield of jerusalem artichoke through upregulation of plant genes under drought stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237377/
https://www.ncbi.nlm.nih.gov/pubmed/37267258
http://dx.doi.org/10.1371/journal.pone.0286625
work_keys_str_mv AT boonmahomepatcha singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress
AT namwongsajunthima singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress
AT vorasootnimitr singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress
AT jogloysanun singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress
AT riddechnuntavan singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress
AT boonluesophon singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress
AT mongkolthanarukwiyada singleandcoinoculumofendophyticbacteriapromotegrowthandyieldofjerusalemartichokethroughupregulationofplantgenesunderdroughtstress