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Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis

Bioremediation is crucial for recuperating polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant-producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present...

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Detalles Bibliográficos
Autores principales: Nayarisseri, Anuraj, Singh, Sanjeev Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237388/
https://www.ncbi.nlm.nih.gov/pubmed/37267268
http://dx.doi.org/10.1371/journal.pone.0285994
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author Nayarisseri, Anuraj
Singh, Sanjeev Kumar
author_facet Nayarisseri, Anuraj
Singh, Sanjeev Kumar
author_sort Nayarisseri, Anuraj
collection PubMed
description Bioremediation is crucial for recuperating polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant-producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present investigation, a biosurfactant-producing bacterium Bacillus tequilensis was isolated from Chilika Lake, Odisha, India (latitude and longitude: 19.8450 N 85.4788 E). Whole-Genome Sequencing (WGS) of Bacillus tequilensis was carried out using Illumina NextSeq 500. The size of the whole genome of Bacillus tequilensis was 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes (ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total raw reads were 4209415, and the processed reads were 4058238 with 4492 genes. The whole genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation, and comparative genome analysis with other existing Bacillus species. In this study, a pathway was constructed which describes the biosurfactant metabolism of Bacillus tequilensis. The study identified that genes such as SrfAD, SrfAC, SrfAA and SrfAB are involved in biosurfactant synthesis. The sequence of the genes SrfAD, SrfAC, SrfAA, SrfAB was deposited in GenBank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole genome sequence was submitted to GenBank with an accession RMVO00000000 and the raw fastq reads were submitted to SRA, NCBI repository with an accession: SRX5023292.
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spelling pubmed-102373882023-06-03 Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis Nayarisseri, Anuraj Singh, Sanjeev Kumar PLoS One Research Article Bioremediation is crucial for recuperating polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant-producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present investigation, a biosurfactant-producing bacterium Bacillus tequilensis was isolated from Chilika Lake, Odisha, India (latitude and longitude: 19.8450 N 85.4788 E). Whole-Genome Sequencing (WGS) of Bacillus tequilensis was carried out using Illumina NextSeq 500. The size of the whole genome of Bacillus tequilensis was 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes (ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total raw reads were 4209415, and the processed reads were 4058238 with 4492 genes. The whole genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation, and comparative genome analysis with other existing Bacillus species. In this study, a pathway was constructed which describes the biosurfactant metabolism of Bacillus tequilensis. The study identified that genes such as SrfAD, SrfAC, SrfAA and SrfAB are involved in biosurfactant synthesis. The sequence of the genes SrfAD, SrfAC, SrfAA, SrfAB was deposited in GenBank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole genome sequence was submitted to GenBank with an accession RMVO00000000 and the raw fastq reads were submitted to SRA, NCBI repository with an accession: SRX5023292. Public Library of Science 2023-06-02 /pmc/articles/PMC10237388/ /pubmed/37267268 http://dx.doi.org/10.1371/journal.pone.0285994 Text en © 2023 Nayarisseri, Singh https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nayarisseri, Anuraj
Singh, Sanjeev Kumar
Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis
title Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis
title_full Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis
title_fullStr Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis
title_full_unstemmed Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis
title_short Genome analysis of biosurfactant producing bacterium, Bacillus tequilensis
title_sort genome analysis of biosurfactant producing bacterium, bacillus tequilensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237388/
https://www.ncbi.nlm.nih.gov/pubmed/37267268
http://dx.doi.org/10.1371/journal.pone.0285994
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