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Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia
PURPOSE: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238078/ https://www.ncbi.nlm.nih.gov/pubmed/37283357 http://dx.doi.org/10.1590/acb381323 |
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author | Abreu, Mateus Souza Tannuri, Ana Cristina Aoun Rodrigues, Rafael Felipe Gonçalves da Silva, Rafael José Gonçalves, Josiane de Oliveira Serafini, Suellen Tannuri, Uenis |
author_facet | Abreu, Mateus Souza Tannuri, Ana Cristina Aoun Rodrigues, Rafael Felipe Gonçalves da Silva, Rafael José Gonçalves, Josiane de Oliveira Serafini, Suellen Tannuri, Uenis |
author_sort | Abreu, Mateus Souza |
collection | PubMed |
description | PURPOSE: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model. METHODS: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses. RESULTS: In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method. CONCLUSIONS: IPoC methods positively reduced the damage caused by IRI in weaning rats. |
format | Online Article Text |
id | pubmed-10238078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
record_format | MEDLINE/PubMed |
spelling | pubmed-102380782023-06-03 Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia Abreu, Mateus Souza Tannuri, Ana Cristina Aoun Rodrigues, Rafael Felipe Gonçalves da Silva, Rafael José Gonçalves, Josiane de Oliveira Serafini, Suellen Tannuri, Uenis Acta Cir Bras Original Article PURPOSE: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model. METHODS: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses. RESULTS: In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method. CONCLUSIONS: IPoC methods positively reduced the damage caused by IRI in weaning rats. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2023-06-02 /pmc/articles/PMC10238078/ /pubmed/37283357 http://dx.doi.org/10.1590/acb381323 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Abreu, Mateus Souza Tannuri, Ana Cristina Aoun Rodrigues, Rafael Felipe Gonçalves da Silva, Rafael José Gonçalves, Josiane de Oliveira Serafini, Suellen Tannuri, Uenis Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
title | Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
title_full | Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
title_fullStr | Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
title_full_unstemmed | Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
title_short | Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
title_sort | effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238078/ https://www.ncbi.nlm.nih.gov/pubmed/37283357 http://dx.doi.org/10.1590/acb381323 |
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