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The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study

BACKGROUND AND AIM: The Duffy (FY) blood group system has six known antigens among which the Fy(a) and Fy(b) are known as major antigens. Fy(x) phenotype forms as a result of two point mutations in the FYB allele leading to instability of Duffy protein and so reduction of Duffy antigen expression in...

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Autores principales: Armstrong‐Fisher, Sylvia, Urbaniak, Stan, Karimi Rouzbahani, Arian, Hemmati Chegeni, Mona, Mahmoudvand, Golnaz, Varzi, Ali Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238784/
https://www.ncbi.nlm.nih.gov/pubmed/37275674
http://dx.doi.org/10.1002/hsr2.1314
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author Armstrong‐Fisher, Sylvia
Urbaniak, Stan
Karimi Rouzbahani, Arian
Hemmati Chegeni, Mona
Mahmoudvand, Golnaz
Varzi, Ali Mohammad
author_facet Armstrong‐Fisher, Sylvia
Urbaniak, Stan
Karimi Rouzbahani, Arian
Hemmati Chegeni, Mona
Mahmoudvand, Golnaz
Varzi, Ali Mohammad
author_sort Armstrong‐Fisher, Sylvia
collection PubMed
description BACKGROUND AND AIM: The Duffy (FY) blood group system has six known antigens among which the Fy(a) and Fy(b) are known as major antigens. Fy(x) phenotype forms as a result of two point mutations in the FYB allele leading to instability of Duffy protein and so reduction of Duffy antigen expression in the cells. This study aimed to investigate the FYX allele frequency in the Scottish population. METHODS: The Duffy blood group system was serologically and molecularly investigated in 222 samples collected from donors of Aberdeen Regional Blood Transfusion Center (BTC). The haemagglutination and BeadChip microarray chemistry methods were used for phenotyping and genotyping. Confirmatory tests were also used to check the discrepant results. RESULTS: In this study, the frequency of Duffy blood group phenotypes including Fy(a+), Fy(a+b+), and Fy(b+) were 17.57%, 42.79%, and 39.64%, respectively. Furthermore, the frequency of FYA/FYA, FYA/FYB, and FYB/FYB genotypes was estimated to be 14.41%, 45.95%, and 39.64%, respectively, using the Bioarray method. In the present study, based on Duffy DNA sequencing results, 12 samples (5.41%) had just one FYX allele. CONCLUSION: The frequency of the FYX allele in this study was estimated to be 0.0270% which is more than the results reported so far.
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spelling pubmed-102387842023-06-04 The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study Armstrong‐Fisher, Sylvia Urbaniak, Stan Karimi Rouzbahani, Arian Hemmati Chegeni, Mona Mahmoudvand, Golnaz Varzi, Ali Mohammad Health Sci Rep Original Research BACKGROUND AND AIM: The Duffy (FY) blood group system has six known antigens among which the Fy(a) and Fy(b) are known as major antigens. Fy(x) phenotype forms as a result of two point mutations in the FYB allele leading to instability of Duffy protein and so reduction of Duffy antigen expression in the cells. This study aimed to investigate the FYX allele frequency in the Scottish population. METHODS: The Duffy blood group system was serologically and molecularly investigated in 222 samples collected from donors of Aberdeen Regional Blood Transfusion Center (BTC). The haemagglutination and BeadChip microarray chemistry methods were used for phenotyping and genotyping. Confirmatory tests were also used to check the discrepant results. RESULTS: In this study, the frequency of Duffy blood group phenotypes including Fy(a+), Fy(a+b+), and Fy(b+) were 17.57%, 42.79%, and 39.64%, respectively. Furthermore, the frequency of FYA/FYA, FYA/FYB, and FYB/FYB genotypes was estimated to be 14.41%, 45.95%, and 39.64%, respectively, using the Bioarray method. In the present study, based on Duffy DNA sequencing results, 12 samples (5.41%) had just one FYX allele. CONCLUSION: The frequency of the FYX allele in this study was estimated to be 0.0270% which is more than the results reported so far. John Wiley and Sons Inc. 2023-06-02 /pmc/articles/PMC10238784/ /pubmed/37275674 http://dx.doi.org/10.1002/hsr2.1314 Text en © 2023 The Authors. Health Science Reports published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Armstrong‐Fisher, Sylvia
Urbaniak, Stan
Karimi Rouzbahani, Arian
Hemmati Chegeni, Mona
Mahmoudvand, Golnaz
Varzi, Ali Mohammad
The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study
title The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study
title_full The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study
title_fullStr The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study
title_full_unstemmed The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study
title_short The highest Duffy (FYX) allele frequency ever reported for Scottish population: A cross‐sectional study
title_sort highest duffy (fyx) allele frequency ever reported for scottish population: a cross‐sectional study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238784/
https://www.ncbi.nlm.nih.gov/pubmed/37275674
http://dx.doi.org/10.1002/hsr2.1314
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