Protocol for early warning of Diaporthe infection in kiwifruit soft rot by plasmonic dimer-enhanced Raman spectroscopy

The pathogens of the Diaporthe genus are now considered to be the dominant pathogens of kiwifruit soft rot. Here, we present a protocol to construct nanoprobes for the Diaporthe genus and detect changes of surface-enhanced Raman spectroscopy for samples collected from infected kiwifruit. We describe...

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Detalles Bibliográficos
Autores principales: Jiang, Hao, Yu, Ting, Miao, Min, Liu, Honglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239012/
https://www.ncbi.nlm.nih.gov/pubmed/37243598
http://dx.doi.org/10.1016/j.xpro.2023.102339
Descripción
Sumario:The pathogens of the Diaporthe genus are now considered to be the dominant pathogens of kiwifruit soft rot. Here, we present a protocol to construct nanoprobes for the Diaporthe genus and detect changes of surface-enhanced Raman spectroscopy for samples collected from infected kiwifruit. We describe steps for synthesis of gold nanoparticles, DNA extraction from kiwifruit, and construction of nanoprobes. We then detail classification of nanoparticles with different aggregation states through dark-field microscope (DFM) picture analysis using Fiji-ImageJ software. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).(1)

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