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D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate

INTRODUCTION: An ideal endogenous molecule for measuring glomerular filtration rate (GFR) is still unknown. However, a rare enantiomer of serine, d-serine, is useful in GFR measurement. This study explored the potential of other d-amino acids for kidney function assessment. METHODS: This was a cross...

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Autores principales: Taniguchi, Ayumu, Kawamura, Masataka, Sakai, Shinsuke, Kimura-Ohba, Shihoko, Tanaka, Yoko, Fukae, Shota, Tanaka, Ryo, Nakazawa, Shigeaki, Yamanaka, Kazuaki, Horio, Masaru, Takahara, Shiro, Nonomura, Norio, Isaka, Yoshitaka, Imamura, Ryoichi, Kimura, Tomonori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239771/
https://www.ncbi.nlm.nih.gov/pubmed/37284685
http://dx.doi.org/10.1016/j.ekir.2023.03.009
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author Taniguchi, Ayumu
Kawamura, Masataka
Sakai, Shinsuke
Kimura-Ohba, Shihoko
Tanaka, Yoko
Fukae, Shota
Tanaka, Ryo
Nakazawa, Shigeaki
Yamanaka, Kazuaki
Horio, Masaru
Takahara, Shiro
Nonomura, Norio
Isaka, Yoshitaka
Imamura, Ryoichi
Kimura, Tomonori
author_facet Taniguchi, Ayumu
Kawamura, Masataka
Sakai, Shinsuke
Kimura-Ohba, Shihoko
Tanaka, Yoko
Fukae, Shota
Tanaka, Ryo
Nakazawa, Shigeaki
Yamanaka, Kazuaki
Horio, Masaru
Takahara, Shiro
Nonomura, Norio
Isaka, Yoshitaka
Imamura, Ryoichi
Kimura, Tomonori
author_sort Taniguchi, Ayumu
collection PubMed
description INTRODUCTION: An ideal endogenous molecule for measuring glomerular filtration rate (GFR) is still unknown. However, a rare enantiomer of serine, d-serine, is useful in GFR measurement. This study explored the potential of other d-amino acids for kidney function assessment. METHODS: This was a cross-sectional observational study of 207 living kidney transplant donors and recipients, for whom GFR was measured using clearance of inulin (C-in). Associations between levels of d-amino acids and GFR were analyzed using multivariate factor analysis. Fractional excretion (FE), a ratio of the clearance of a substance to C-in as a standard molecule, was calculated to monitor the excretion ratio after glomerular filtration. Dissociation from an ideal FE of 100% was assessed as a bias. Proportional bias against C-in was calculated using Deming regression. RESULTS: Multivariate analysis identified the blood level of d-asparagine to reflect GFR. Means of blood d-asparagine and clearance of d-asparagine (C-d-Asn) were 0.21 μM and 65.0 ml/min per 1.73 m(2), respectively. Inulin-based FE (FE(in)) of d-asparagine was 98.67% (95% confidence interval [CI]: 96.43–100.90%) and less biased than those of known GFR markers, such as FE(in) of creatinine (147.93 [145.39–150.46]; P < 0.001) and d-serine (84.84 [83.22–86.46]; P < 0.001). A proportional bias of C-d-Asn to C-in was −7.8% (95% CI, −14.5 to −0.6%), which was minor compared to those of clearance of creatinine (−34.5% [−37.9 to −31.0%]) and d-serine (21.2% [13.9–28.9]). CONCLUSION: D-Asparagine acts similar to inulin in the kidney. Therefore, d-asparagine is an ideal endogenous molecule that can be used for GFR measurement.
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spelling pubmed-102397712023-06-06 D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate Taniguchi, Ayumu Kawamura, Masataka Sakai, Shinsuke Kimura-Ohba, Shihoko Tanaka, Yoko Fukae, Shota Tanaka, Ryo Nakazawa, Shigeaki Yamanaka, Kazuaki Horio, Masaru Takahara, Shiro Nonomura, Norio Isaka, Yoshitaka Imamura, Ryoichi Kimura, Tomonori Kidney Int Rep Clinical Research INTRODUCTION: An ideal endogenous molecule for measuring glomerular filtration rate (GFR) is still unknown. However, a rare enantiomer of serine, d-serine, is useful in GFR measurement. This study explored the potential of other d-amino acids for kidney function assessment. METHODS: This was a cross-sectional observational study of 207 living kidney transplant donors and recipients, for whom GFR was measured using clearance of inulin (C-in). Associations between levels of d-amino acids and GFR were analyzed using multivariate factor analysis. Fractional excretion (FE), a ratio of the clearance of a substance to C-in as a standard molecule, was calculated to monitor the excretion ratio after glomerular filtration. Dissociation from an ideal FE of 100% was assessed as a bias. Proportional bias against C-in was calculated using Deming regression. RESULTS: Multivariate analysis identified the blood level of d-asparagine to reflect GFR. Means of blood d-asparagine and clearance of d-asparagine (C-d-Asn) were 0.21 μM and 65.0 ml/min per 1.73 m(2), respectively. Inulin-based FE (FE(in)) of d-asparagine was 98.67% (95% confidence interval [CI]: 96.43–100.90%) and less biased than those of known GFR markers, such as FE(in) of creatinine (147.93 [145.39–150.46]; P < 0.001) and d-serine (84.84 [83.22–86.46]; P < 0.001). A proportional bias of C-d-Asn to C-in was −7.8% (95% CI, −14.5 to −0.6%), which was minor compared to those of clearance of creatinine (−34.5% [−37.9 to −31.0%]) and d-serine (21.2% [13.9–28.9]). CONCLUSION: D-Asparagine acts similar to inulin in the kidney. Therefore, d-asparagine is an ideal endogenous molecule that can be used for GFR measurement. Elsevier 2023-03-21 /pmc/articles/PMC10239771/ /pubmed/37284685 http://dx.doi.org/10.1016/j.ekir.2023.03.009 Text en © 2023 International Society of Nephrology. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Clinical Research
Taniguchi, Ayumu
Kawamura, Masataka
Sakai, Shinsuke
Kimura-Ohba, Shihoko
Tanaka, Yoko
Fukae, Shota
Tanaka, Ryo
Nakazawa, Shigeaki
Yamanaka, Kazuaki
Horio, Masaru
Takahara, Shiro
Nonomura, Norio
Isaka, Yoshitaka
Imamura, Ryoichi
Kimura, Tomonori
D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate
title D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate
title_full D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate
title_fullStr D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate
title_full_unstemmed D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate
title_short D-Asparagine is an Ideal Endogenous Molecule for Measuring the Glomerular Filtration Rate
title_sort d-asparagine is an ideal endogenous molecule for measuring the glomerular filtration rate
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239771/
https://www.ncbi.nlm.nih.gov/pubmed/37284685
http://dx.doi.org/10.1016/j.ekir.2023.03.009
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