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Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics
INTRODUCTION: Humic substances (HSs), components of plant biostimulants, are known to influence plant physiological processes, nutrient uptake and plant growth, thereby increasing crop yield. However, few studies have focused on the impact of HS on overall plant metabolism, and there is still debate...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239833/ https://www.ncbi.nlm.nih.gov/pubmed/37284724 http://dx.doi.org/10.3389/fpls.2023.1122621 |
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author | Wang, Yuhong Lu, Yanli Wang, Lei Song, Guipei Ni, Lu Xu, Mengze Nie, Caie Li, Baoguo Bai, Youlu |
author_facet | Wang, Yuhong Lu, Yanli Wang, Lei Song, Guipei Ni, Lu Xu, Mengze Nie, Caie Li, Baoguo Bai, Youlu |
author_sort | Wang, Yuhong |
collection | PubMed |
description | INTRODUCTION: Humic substances (HSs), components of plant biostimulants, are known to influence plant physiological processes, nutrient uptake and plant growth, thereby increasing crop yield. However, few studies have focused on the impact of HS on overall plant metabolism, and there is still debate over the connection between HS’ structural characteristics and their stimulatory actions. METHODS: In this study, two different HSs (AHA, Aojia humic acid and SHA, Shandong humic acid) screened in a previous experiment were chosen for foliar spraying, and plant samples were collected on the tenth day after spraying (62 days after germination) to investigate the effects of different HSs on photosynthesis, dry matter accumulation, carbon and nitrogen metabolism and overall metabolism in maize leaf. RESULTS AND DISCUSSION: The results showed different molecular compositions for AHA and SHA and a total of 510 small molecules with significant differences were screened using an ESI-OPLC-MS techno. AHA and SHA exerted different effects on maize growth, with the AHA inducing more effective stimulation than the SHA doing. Untargeted metabolomic analysis revealed that the phospholipid components of maize leaves treated by SHA generally increased significantly than that in the AHA and control treatments. Additionally, both HS-treated maize leaves exhibited different levels of accumulation of trans-zeatin, but SHA treatment significantly decreased the accumulation of zeatin riboside. Compared to CK treatment, AHA treatment resulted in the reorganization of four metabolic pathways: starch and sucrose metabolism, TCA cycle, stilbenes, diarylheptanes, and curcumin biosynthesis, and ABC transport, SHA treatment modified starch and sucrose metabolism and unsaturated fatty acid biosynthesis. These results demonstrate that HSs exert their function through a multifaceted mechanism of action, partially connected to their hormone-like activity but also involving hormoneindependent signaling pathways. |
format | Online Article Text |
id | pubmed-10239833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102398332023-06-06 Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics Wang, Yuhong Lu, Yanli Wang, Lei Song, Guipei Ni, Lu Xu, Mengze Nie, Caie Li, Baoguo Bai, Youlu Front Plant Sci Plant Science INTRODUCTION: Humic substances (HSs), components of plant biostimulants, are known to influence plant physiological processes, nutrient uptake and plant growth, thereby increasing crop yield. However, few studies have focused on the impact of HS on overall plant metabolism, and there is still debate over the connection between HS’ structural characteristics and their stimulatory actions. METHODS: In this study, two different HSs (AHA, Aojia humic acid and SHA, Shandong humic acid) screened in a previous experiment were chosen for foliar spraying, and plant samples were collected on the tenth day after spraying (62 days after germination) to investigate the effects of different HSs on photosynthesis, dry matter accumulation, carbon and nitrogen metabolism and overall metabolism in maize leaf. RESULTS AND DISCUSSION: The results showed different molecular compositions for AHA and SHA and a total of 510 small molecules with significant differences were screened using an ESI-OPLC-MS techno. AHA and SHA exerted different effects on maize growth, with the AHA inducing more effective stimulation than the SHA doing. Untargeted metabolomic analysis revealed that the phospholipid components of maize leaves treated by SHA generally increased significantly than that in the AHA and control treatments. Additionally, both HS-treated maize leaves exhibited different levels of accumulation of trans-zeatin, but SHA treatment significantly decreased the accumulation of zeatin riboside. Compared to CK treatment, AHA treatment resulted in the reorganization of four metabolic pathways: starch and sucrose metabolism, TCA cycle, stilbenes, diarylheptanes, and curcumin biosynthesis, and ABC transport, SHA treatment modified starch and sucrose metabolism and unsaturated fatty acid biosynthesis. These results demonstrate that HSs exert their function through a multifaceted mechanism of action, partially connected to their hormone-like activity but also involving hormoneindependent signaling pathways. Frontiers Media S.A. 2023-05-22 /pmc/articles/PMC10239833/ /pubmed/37284724 http://dx.doi.org/10.3389/fpls.2023.1122621 Text en Copyright © 2023 Wang, Lu, Wang, Song, Ni, Xu, Nie, Li and Bai https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Yuhong Lu, Yanli Wang, Lei Song, Guipei Ni, Lu Xu, Mengze Nie, Caie Li, Baoguo Bai, Youlu Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
title | Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
title_full | Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
title_fullStr | Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
title_full_unstemmed | Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
title_short | Analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
title_sort | analysis of the molecular composition of humic substances and their effects on physiological metabolism in maize based on untargeted metabolomics |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239833/ https://www.ncbi.nlm.nih.gov/pubmed/37284724 http://dx.doi.org/10.3389/fpls.2023.1122621 |
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