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Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction
Introduction: Methionine (Met) is an important amino acid related to the development of skeletal muscle. This study investigated the effects of dietary Met restriction on the gene expression of M. iliotibialis lateralis. Methods: A total of 84 day-old broiler chicks (Zhuanghe Dagu) with a similar in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240061/ https://www.ncbi.nlm.nih.gov/pubmed/37284544 http://dx.doi.org/10.3389/fphys.2023.1184651 |
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author | Li, Desheng Chen, Fei Tian, Yumin Su, Yuhong |
author_facet | Li, Desheng Chen, Fei Tian, Yumin Su, Yuhong |
author_sort | Li, Desheng |
collection | PubMed |
description | Introduction: Methionine (Met) is an important amino acid related to the development of skeletal muscle. This study investigated the effects of dietary Met restriction on the gene expression of M. iliotibialis lateralis. Methods: A total of 84 day-old broiler chicks (Zhuanghe Dagu) with a similar initial body weight (207.62 ± 8.54 g) were used in this study. All birds were divided into two groups (CON; L-Met) based on the initial body weight. Each group consisted of six replicates with seven birds per replicate. The experimental period was 63 days (phase 1, days 1-21; phase 2, days 22-63). According to the nutritional requirements of Zhuanghe Dagu chickens, we provided a basal diet (0.39% Met levels during phase 1 and 0.35% Met levels during phase 2, as-fed basis) to the birds in the CON group, while we provided a Met-restricted diet (0.31% Met levels during phase 1 and 0.28% Met levels during phase 2, as-fed basis) to the birds in the L-Met group. The growth performance of broiler chicks and their M. iliotibialis lateralis development parameters were measured on days 21 and 63. Results and Discussion: In this study, dietary Met restriction did not affect the growth performance of broiler chicks but hindered the development of M. iliotibialis lateralis at both sampling timepoints. On the final day, three birds selected from each group (three from CON and three from L-Met) were used to obtain M. iliotibialis lateralis samples from leg muscle for further transcriptome analysis. Transcriptome analysis revealed that dietary Met restriction significantly upregulated 247 differentially expressed genes (DEGs) and downregulated 173 DEGs. Additionally, DEGs were mainly enriched in 10 pathways. Among DEGs, we observed that dietary Met restriction downregulated the expression of CSRP3, KY, FHL1, LMCD1, and MYOZ2 in M. iliotibialis lateralis. Therefore, we considered that dietary Met restriction had negative effects on the development of M. iliotibialis lateralis, and CSRP3, KY, FHL1, LMCD1, and MYOZ2 may serve as potential functional genes involved in this process. |
format | Online Article Text |
id | pubmed-10240061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102400612023-06-06 Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction Li, Desheng Chen, Fei Tian, Yumin Su, Yuhong Front Physiol Physiology Introduction: Methionine (Met) is an important amino acid related to the development of skeletal muscle. This study investigated the effects of dietary Met restriction on the gene expression of M. iliotibialis lateralis. Methods: A total of 84 day-old broiler chicks (Zhuanghe Dagu) with a similar initial body weight (207.62 ± 8.54 g) were used in this study. All birds were divided into two groups (CON; L-Met) based on the initial body weight. Each group consisted of six replicates with seven birds per replicate. The experimental period was 63 days (phase 1, days 1-21; phase 2, days 22-63). According to the nutritional requirements of Zhuanghe Dagu chickens, we provided a basal diet (0.39% Met levels during phase 1 and 0.35% Met levels during phase 2, as-fed basis) to the birds in the CON group, while we provided a Met-restricted diet (0.31% Met levels during phase 1 and 0.28% Met levels during phase 2, as-fed basis) to the birds in the L-Met group. The growth performance of broiler chicks and their M. iliotibialis lateralis development parameters were measured on days 21 and 63. Results and Discussion: In this study, dietary Met restriction did not affect the growth performance of broiler chicks but hindered the development of M. iliotibialis lateralis at both sampling timepoints. On the final day, three birds selected from each group (three from CON and three from L-Met) were used to obtain M. iliotibialis lateralis samples from leg muscle for further transcriptome analysis. Transcriptome analysis revealed that dietary Met restriction significantly upregulated 247 differentially expressed genes (DEGs) and downregulated 173 DEGs. Additionally, DEGs were mainly enriched in 10 pathways. Among DEGs, we observed that dietary Met restriction downregulated the expression of CSRP3, KY, FHL1, LMCD1, and MYOZ2 in M. iliotibialis lateralis. Therefore, we considered that dietary Met restriction had negative effects on the development of M. iliotibialis lateralis, and CSRP3, KY, FHL1, LMCD1, and MYOZ2 may serve as potential functional genes involved in this process. Frontiers Media S.A. 2023-05-22 /pmc/articles/PMC10240061/ /pubmed/37284544 http://dx.doi.org/10.3389/fphys.2023.1184651 Text en Copyright © 2023 Li, Chen, Tian and Su. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Li, Desheng Chen, Fei Tian, Yumin Su, Yuhong Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction |
title | Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction |
title_full | Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction |
title_fullStr | Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction |
title_full_unstemmed | Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction |
title_short | Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction |
title_sort | transcriptome analysis of the gene expression of m. iliotibialis lateralis affected by dietary methionine restriction |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240061/ https://www.ncbi.nlm.nih.gov/pubmed/37284544 http://dx.doi.org/10.3389/fphys.2023.1184651 |
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