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Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells

5‐Fluorouracil (5‐FU) is widely used for colorectal cancer (CRC) treatment; however, continuous treatment of CRC cells with 5‐FU can result in acquired resistance, and the underlying mechanism of 5‐FU resistance remains unclear. We previously established an acquired 5‐FU‐resistant CRC cell line, HCT...

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Autores principales: Kurasaka, Chinatsu, Nishizawa, Nana, Uozumi, Haruka, Ogino, Yoko, Sato, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240340/
https://www.ncbi.nlm.nih.gov/pubmed/37058332
http://dx.doi.org/10.1002/2211-5463.13611
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author Kurasaka, Chinatsu
Nishizawa, Nana
Uozumi, Haruka
Ogino, Yoko
Sato, Akira
author_facet Kurasaka, Chinatsu
Nishizawa, Nana
Uozumi, Haruka
Ogino, Yoko
Sato, Akira
author_sort Kurasaka, Chinatsu
collection PubMed
description 5‐Fluorouracil (5‐FU) is widely used for colorectal cancer (CRC) treatment; however, continuous treatment of CRC cells with 5‐FU can result in acquired resistance, and the underlying mechanism of 5‐FU resistance remains unclear. We previously established an acquired 5‐FU‐resistant CRC cell line, HCT116R(F10), and examined its biological features and 5‐FU resistance mechanisms. In this study, we evaluated the 5‐FU sensitivity and cellular respiration dependency of HCT116R(F10) cells and parental HCT116 cells under conditions of high‐ and low‐glucose concentrations. Both HCT116R(F10) and parental HCT116 cells were more sensitive to 5‐FU under low‐glucose conditions compared with high‐glucose conditions. Interestingly, HCT116R(F10) and parental HCT116 cells exhibited altered cellular respiration dependence for glycolysis and mitochondrial respiration under high‐ and low‐glucose conditions. Additionally, HCT116R(F10) cells showed a markedly decreased ATP production rate compared with HCT116 cells under both high‐ and low‐glucose conditions. Importantly, glucose restriction significantly reduced the ATP production rate for both glycolysis and mitochondrial respiration in HCT116R(F10) cells compared with HCT116 cells. The ATP production rates in HCT116R(F10) and HCT116 cells were reduced by approximately 64% and 23%, respectively, under glucose restriction, suggesting that glucose restriction may be effective at enhancing 5‐FU chemotherapy. Overall, these findings shed light on 5‐FU resistance mechanisms, which may lead to improvements in anticancer treatment strategies.
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spelling pubmed-102403402023-06-06 Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells Kurasaka, Chinatsu Nishizawa, Nana Uozumi, Haruka Ogino, Yoko Sato, Akira FEBS Open Bio Research Articles 5‐Fluorouracil (5‐FU) is widely used for colorectal cancer (CRC) treatment; however, continuous treatment of CRC cells with 5‐FU can result in acquired resistance, and the underlying mechanism of 5‐FU resistance remains unclear. We previously established an acquired 5‐FU‐resistant CRC cell line, HCT116R(F10), and examined its biological features and 5‐FU resistance mechanisms. In this study, we evaluated the 5‐FU sensitivity and cellular respiration dependency of HCT116R(F10) cells and parental HCT116 cells under conditions of high‐ and low‐glucose concentrations. Both HCT116R(F10) and parental HCT116 cells were more sensitive to 5‐FU under low‐glucose conditions compared with high‐glucose conditions. Interestingly, HCT116R(F10) and parental HCT116 cells exhibited altered cellular respiration dependence for glycolysis and mitochondrial respiration under high‐ and low‐glucose conditions. Additionally, HCT116R(F10) cells showed a markedly decreased ATP production rate compared with HCT116 cells under both high‐ and low‐glucose conditions. Importantly, glucose restriction significantly reduced the ATP production rate for both glycolysis and mitochondrial respiration in HCT116R(F10) cells compared with HCT116 cells. The ATP production rates in HCT116R(F10) and HCT116 cells were reduced by approximately 64% and 23%, respectively, under glucose restriction, suggesting that glucose restriction may be effective at enhancing 5‐FU chemotherapy. Overall, these findings shed light on 5‐FU resistance mechanisms, which may lead to improvements in anticancer treatment strategies. John Wiley and Sons Inc. 2023-04-19 /pmc/articles/PMC10240340/ /pubmed/37058332 http://dx.doi.org/10.1002/2211-5463.13611 Text en © 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Kurasaka, Chinatsu
Nishizawa, Nana
Uozumi, Haruka
Ogino, Yoko
Sato, Akira
Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells
title Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells
title_full Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells
title_fullStr Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells
title_full_unstemmed Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells
title_short Relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant HCT116 human colorectal cancer cells
title_sort relationship between anticancer sensitivities and cellular respiration properties in 5‐fluorouracil‐resistant hct116 human colorectal cancer cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240340/
https://www.ncbi.nlm.nih.gov/pubmed/37058332
http://dx.doi.org/10.1002/2211-5463.13611
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