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The single-cell and spatial transcriptional landscape of human gastrulation and early brain development

The emergence of the three germ layers and the lineage-specific precursor cells orchestrating organogenesis represent fundamental milestones during early embryonic development. We analyzed the transcriptional profiles of over 400,000 cells from 14 human samples collected from post-conceptional weeks...

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Autores principales: Zeng, Bo, Liu, Zeyuan, Lu, Yufeng, Zhong, Suijuan, Qin, Shenyue, Huang, Luwei, Zeng, Yang, Li, Zixiao, Dong, Hao, Shi, Yingchao, Yang, Jialei, Dai, Yalun, Ma, Qiang, Sun, Le, Bian, Lihong, Han, Dan, Chen, Youqiao, Qiu, Xin, Wang, Wei, Marín, Oscar, Wu, Qian, Wang, Yongjun, Wang, Xiaoqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241223/
https://www.ncbi.nlm.nih.gov/pubmed/37192616
http://dx.doi.org/10.1016/j.stem.2023.04.016
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author Zeng, Bo
Liu, Zeyuan
Lu, Yufeng
Zhong, Suijuan
Qin, Shenyue
Huang, Luwei
Zeng, Yang
Li, Zixiao
Dong, Hao
Shi, Yingchao
Yang, Jialei
Dai, Yalun
Ma, Qiang
Sun, Le
Bian, Lihong
Han, Dan
Chen, Youqiao
Qiu, Xin
Wang, Wei
Marín, Oscar
Wu, Qian
Wang, Yongjun
Wang, Xiaoqun
author_facet Zeng, Bo
Liu, Zeyuan
Lu, Yufeng
Zhong, Suijuan
Qin, Shenyue
Huang, Luwei
Zeng, Yang
Li, Zixiao
Dong, Hao
Shi, Yingchao
Yang, Jialei
Dai, Yalun
Ma, Qiang
Sun, Le
Bian, Lihong
Han, Dan
Chen, Youqiao
Qiu, Xin
Wang, Wei
Marín, Oscar
Wu, Qian
Wang, Yongjun
Wang, Xiaoqun
author_sort Zeng, Bo
collection PubMed
description The emergence of the three germ layers and the lineage-specific precursor cells orchestrating organogenesis represent fundamental milestones during early embryonic development. We analyzed the transcriptional profiles of over 400,000 cells from 14 human samples collected from post-conceptional weeks (PCW) 3 to 12 to delineate the dynamic molecular and cellular landscape of early gastrulation and nervous system development. We described the diversification of cell types, the spatial patterning of neural tube cells, and the signaling pathways likely involved in transforming epiblast cells into neuroepithelial cells and then into radial glia. We resolved 24 clusters of radial glial cells along the neural tube and outlined differentiation trajectories for the main classes of neurons. Lastly, we identified conserved and distinctive features across species by comparing early embryonic single-cell transcriptomic profiles between humans and mice. This comprehensive atlas sheds light on the molecular mechanisms underlying gastrulation and early human brain development.
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spelling pubmed-102412232023-06-06 The single-cell and spatial transcriptional landscape of human gastrulation and early brain development Zeng, Bo Liu, Zeyuan Lu, Yufeng Zhong, Suijuan Qin, Shenyue Huang, Luwei Zeng, Yang Li, Zixiao Dong, Hao Shi, Yingchao Yang, Jialei Dai, Yalun Ma, Qiang Sun, Le Bian, Lihong Han, Dan Chen, Youqiao Qiu, Xin Wang, Wei Marín, Oscar Wu, Qian Wang, Yongjun Wang, Xiaoqun Cell Stem Cell Resource The emergence of the three germ layers and the lineage-specific precursor cells orchestrating organogenesis represent fundamental milestones during early embryonic development. We analyzed the transcriptional profiles of over 400,000 cells from 14 human samples collected from post-conceptional weeks (PCW) 3 to 12 to delineate the dynamic molecular and cellular landscape of early gastrulation and nervous system development. We described the diversification of cell types, the spatial patterning of neural tube cells, and the signaling pathways likely involved in transforming epiblast cells into neuroepithelial cells and then into radial glia. We resolved 24 clusters of radial glial cells along the neural tube and outlined differentiation trajectories for the main classes of neurons. Lastly, we identified conserved and distinctive features across species by comparing early embryonic single-cell transcriptomic profiles between humans and mice. This comprehensive atlas sheds light on the molecular mechanisms underlying gastrulation and early human brain development. Cell Press 2023-06-01 /pmc/articles/PMC10241223/ /pubmed/37192616 http://dx.doi.org/10.1016/j.stem.2023.04.016 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Resource
Zeng, Bo
Liu, Zeyuan
Lu, Yufeng
Zhong, Suijuan
Qin, Shenyue
Huang, Luwei
Zeng, Yang
Li, Zixiao
Dong, Hao
Shi, Yingchao
Yang, Jialei
Dai, Yalun
Ma, Qiang
Sun, Le
Bian, Lihong
Han, Dan
Chen, Youqiao
Qiu, Xin
Wang, Wei
Marín, Oscar
Wu, Qian
Wang, Yongjun
Wang, Xiaoqun
The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
title The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
title_full The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
title_fullStr The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
title_full_unstemmed The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
title_short The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
title_sort single-cell and spatial transcriptional landscape of human gastrulation and early brain development
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241223/
https://www.ncbi.nlm.nih.gov/pubmed/37192616
http://dx.doi.org/10.1016/j.stem.2023.04.016
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