CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice

AIM: This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice. MATERIALS & METHODS: We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodie...

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Autores principales: Liu, Jiandong, Cang, Tianle, Jiang, Congli, Li, Kelei, Liu, Siyuan, Wang, Haixin, Wang, Meirong, Chen, Yan, Shao, Yan, Liu, Jiankai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241461/
http://dx.doi.org/10.2217/fvl-2022-0172
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author Liu, Jiandong
Cang, Tianle
Jiang, Congli
Li, Kelei
Liu, Siyuan
Wang, Haixin
Wang, Meirong
Chen, Yan
Shao, Yan
Liu, Jiankai
author_facet Liu, Jiandong
Cang, Tianle
Jiang, Congli
Li, Kelei
Liu, Siyuan
Wang, Haixin
Wang, Meirong
Chen, Yan
Shao, Yan
Liu, Jiankai
author_sort Liu, Jiandong
collection PubMed
description AIM: This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice. MATERIALS & METHODS: We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodies were detected in serum by ELISA. The IFN-γ cytokine was detected by ELISPOT. RESULTS: CpG 684 improved spike-specific IgG and IgM subtype binding antibodies and increased the neutralizing antibody titer against prototype, Delta and Beta strains. CpG 684 also improved cellular immune response. CONCLUSION: CpG 684 is an effective adjuvant for inactivated COVID-19 vaccine.
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spelling pubmed-102414612023-06-06 CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice Liu, Jiandong Cang, Tianle Jiang, Congli Li, Kelei Liu, Siyuan Wang, Haixin Wang, Meirong Chen, Yan Shao, Yan Liu, Jiankai Future Virol Short Communication AIM: This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice. MATERIALS & METHODS: We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodies were detected in serum by ELISA. The IFN-γ cytokine was detected by ELISPOT. RESULTS: CpG 684 improved spike-specific IgG and IgM subtype binding antibodies and increased the neutralizing antibody titer against prototype, Delta and Beta strains. CpG 684 also improved cellular immune response. CONCLUSION: CpG 684 is an effective adjuvant for inactivated COVID-19 vaccine. Future Medicine Ltd 2023-06-01 2023-05 /pmc/articles/PMC10241461/ http://dx.doi.org/10.2217/fvl-2022-0172 Text en © 2023 Future Medicine Ltd https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Short Communication
Liu, Jiandong
Cang, Tianle
Jiang, Congli
Li, Kelei
Liu, Siyuan
Wang, Haixin
Wang, Meirong
Chen, Yan
Shao, Yan
Liu, Jiankai
CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice
title CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice
title_full CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice
title_fullStr CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice
title_full_unstemmed CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice
title_short CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice
title_sort cpg 684: an effective adjuvant for the inactivated covid-19 vaccine in mice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241461/
http://dx.doi.org/10.2217/fvl-2022-0172
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