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Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel
Diabetes mellitus is associated with impaired wound healing. The topical use of insulin is a promising therapy because it may favor all phases of the wound healing process. This study aimed to investigate the therapeutic outcomes of insulin gel in wounds of hyperglycemic mice. After diabetes inducti...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241483/ https://www.ncbi.nlm.nih.gov/pubmed/37194835 http://dx.doi.org/10.1590/1414-431X2023e12640 |
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author | Apolinário, P.P. Zanchetta, F.C. Breder, J.S.C. Adams, G. Consonni, S.R. Gillis, R. Saad, M.J.A. Lima, M.H.M. |
author_facet | Apolinário, P.P. Zanchetta, F.C. Breder, J.S.C. Adams, G. Consonni, S.R. Gillis, R. Saad, M.J.A. Lima, M.H.M. |
author_sort | Apolinário, P.P. |
collection | PubMed |
description | Diabetes mellitus is associated with impaired wound healing. The topical use of insulin is a promising therapy because it may favor all phases of the wound healing process. This study aimed to investigate the therapeutic outcomes of insulin gel in wounds of hyperglycemic mice. After diabetes induction, a 1-cm(2) full-thickness wound was created on each animal's dorsum. The lesions were treated daily for 14 days with insulin gel (insulin group) or vehicle gel without insulin (vehicle group). Tissue samples were extracted on days 4, 7, 10, and 14 after the creation of the lesion. The samples were analyzed with hematoxylin/eosin and Sirius red staining, immunohistochemistry, Bio-Plex immunoassays, and western blotting. Insulin gel favored re-epithelialization at day 10 and increased the organization and deposition of collagen. Additionally, it modulated the expression of cytokines (interleukin (IL)-4 and IL-10) and increased the expression of arginase I, VEGF receptor 1, and VEGF on day 10. Activation of the insulin signaling pathway occurred via IRβ, IRS1, and IKK on day 10 and activation of Akt and IRS1 on day 14. These results suggested that insulin gel improved wound healing in hyperglycemic mice by modulating the expression of inflammatory factors, growth factors, and proteins of the insulin signaling pathway. |
format | Online Article Text |
id | pubmed-10241483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-102414832023-06-06 Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel Apolinário, P.P. Zanchetta, F.C. Breder, J.S.C. Adams, G. Consonni, S.R. Gillis, R. Saad, M.J.A. Lima, M.H.M. Braz J Med Biol Res Research Article Diabetes mellitus is associated with impaired wound healing. The topical use of insulin is a promising therapy because it may favor all phases of the wound healing process. This study aimed to investigate the therapeutic outcomes of insulin gel in wounds of hyperglycemic mice. After diabetes induction, a 1-cm(2) full-thickness wound was created on each animal's dorsum. The lesions were treated daily for 14 days with insulin gel (insulin group) or vehicle gel without insulin (vehicle group). Tissue samples were extracted on days 4, 7, 10, and 14 after the creation of the lesion. The samples were analyzed with hematoxylin/eosin and Sirius red staining, immunohistochemistry, Bio-Plex immunoassays, and western blotting. Insulin gel favored re-epithelialization at day 10 and increased the organization and deposition of collagen. Additionally, it modulated the expression of cytokines (interleukin (IL)-4 and IL-10) and increased the expression of arginase I, VEGF receptor 1, and VEGF on day 10. Activation of the insulin signaling pathway occurred via IRβ, IRS1, and IKK on day 10 and activation of Akt and IRS1 on day 14. These results suggested that insulin gel improved wound healing in hyperglycemic mice by modulating the expression of inflammatory factors, growth factors, and proteins of the insulin signaling pathway. Associação Brasileira de Divulgação Científica 2023-05-15 /pmc/articles/PMC10241483/ /pubmed/37194835 http://dx.doi.org/10.1590/1414-431X2023e12640 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Apolinário, P.P. Zanchetta, F.C. Breder, J.S.C. Adams, G. Consonni, S.R. Gillis, R. Saad, M.J.A. Lima, M.H.M. Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
title | Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
title_full | Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
title_fullStr | Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
title_full_unstemmed | Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
title_short | Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
title_sort | anti-inflammatory, procollagen, and wound repair properties of topical insulin gel |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241483/ https://www.ncbi.nlm.nih.gov/pubmed/37194835 http://dx.doi.org/10.1590/1414-431X2023e12640 |
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