Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells

We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and d...

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Detalles Bibliográficos
Autores principales: Kong, Nannan, Chan, Ying Wai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241970/
https://www.ncbi.nlm.nih.gov/pubmed/37252842
http://dx.doi.org/10.1016/j.xpro.2023.102286

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241970/
https://www.ncbi.nlm.nih.gov/pubmed/37252842
http://dx.doi.org/10.1016/j.xpro.2023.102286

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