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Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells
We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and d...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241970/ https://www.ncbi.nlm.nih.gov/pubmed/37252842 http://dx.doi.org/10.1016/j.xpro.2023.102286 |