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Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor
OBJECTIVE: Acyl-CoA-binding protein (ACBP)/diazepam-binding inhibitor has lately been described as an endocrine factor affecting food intake and lipid metabolism. ACBP is dysregulated in catabolic/malnutrition states like sepsis or systemic inflammation. However, regulation of ACBP has not been inve...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10242139/ https://www.ncbi.nlm.nih.gov/pubmed/37288304 http://dx.doi.org/10.3389/fendo.2023.1152444 |
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author | Schürfeld, Robin Sandner, Benjamin Hoffmann, Annett Klöting, Nora Baratashvili, Ekaterine Nowicki, Marcin Paeschke, Sabine Kosacka, Joanna Kralisch, Susan Bachmann, Anette Frille, Armin Dietel, Anja Stolzenburg, Jens-Uwe Blüher, Matthias Zhang, Ming-Zhi Harris, Raymond C. Isermann, Berend Stumvoll, Michael Tönjes, Anke Ebert, Thomas |
author_facet | Schürfeld, Robin Sandner, Benjamin Hoffmann, Annett Klöting, Nora Baratashvili, Ekaterine Nowicki, Marcin Paeschke, Sabine Kosacka, Joanna Kralisch, Susan Bachmann, Anette Frille, Armin Dietel, Anja Stolzenburg, Jens-Uwe Blüher, Matthias Zhang, Ming-Zhi Harris, Raymond C. Isermann, Berend Stumvoll, Michael Tönjes, Anke Ebert, Thomas |
author_sort | Schürfeld, Robin |
collection | PubMed |
description | OBJECTIVE: Acyl-CoA-binding protein (ACBP)/diazepam-binding inhibitor has lately been described as an endocrine factor affecting food intake and lipid metabolism. ACBP is dysregulated in catabolic/malnutrition states like sepsis or systemic inflammation. However, regulation of ACBP has not been investigated in conditions with impaired kidney function, so far. DESIGN/METHODS: Serum ACBP concentrations were investigated by enzyme-linked immunosorbent assay i) in a cohort of 60 individuals with kidney failure (KF) on chronic haemodialysis and compared to 60 individuals with a preserved kidney function; and ii) in a human model of acute kidney dysfunction (AKD). In addition, mACBP mRNA expression was assessed in two CKD mouse models and in two distinct groups of non-CKD mice. Further, mRNA expression of mACBP was measured in vitro in isolated, differentiated mouse adipocytes - brown and white - after exposure to the uremic agent indoxyl sulfate. RESULTS: Median [interquartile range] serum ACBP was almost 20-fold increased in KF (514.0 [339.3] µg/l) compared to subjects without KF (26.1 [39.1] µg/l) (p<0.001). eGFR was the most important, inverse predictor of circulating ACBP in multivariate analysis (standardized β=-0.839; p<0.001). Furthermore, AKD increased ACBP concentrations almost 3-fold (p<0.001). Increased ACBP levels were not caused by augmented mACBP mRNA expression in different tissues of CKD mice in vivo or in indoxyl sulfate-treated adipocytes in vitro. CONCLUSIONS: Circulating ACBP inversely associates with renal function, most likely through renal retention of the cytokine. Future studies need to investigate ACBP physiology in malnutrition-related disease states, such as CKD, and to adjust for markers of renal function. |
format | Online Article Text |
id | pubmed-10242139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102421392023-06-07 Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor Schürfeld, Robin Sandner, Benjamin Hoffmann, Annett Klöting, Nora Baratashvili, Ekaterine Nowicki, Marcin Paeschke, Sabine Kosacka, Joanna Kralisch, Susan Bachmann, Anette Frille, Armin Dietel, Anja Stolzenburg, Jens-Uwe Blüher, Matthias Zhang, Ming-Zhi Harris, Raymond C. Isermann, Berend Stumvoll, Michael Tönjes, Anke Ebert, Thomas Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: Acyl-CoA-binding protein (ACBP)/diazepam-binding inhibitor has lately been described as an endocrine factor affecting food intake and lipid metabolism. ACBP is dysregulated in catabolic/malnutrition states like sepsis or systemic inflammation. However, regulation of ACBP has not been investigated in conditions with impaired kidney function, so far. DESIGN/METHODS: Serum ACBP concentrations were investigated by enzyme-linked immunosorbent assay i) in a cohort of 60 individuals with kidney failure (KF) on chronic haemodialysis and compared to 60 individuals with a preserved kidney function; and ii) in a human model of acute kidney dysfunction (AKD). In addition, mACBP mRNA expression was assessed in two CKD mouse models and in two distinct groups of non-CKD mice. Further, mRNA expression of mACBP was measured in vitro in isolated, differentiated mouse adipocytes - brown and white - after exposure to the uremic agent indoxyl sulfate. RESULTS: Median [interquartile range] serum ACBP was almost 20-fold increased in KF (514.0 [339.3] µg/l) compared to subjects without KF (26.1 [39.1] µg/l) (p<0.001). eGFR was the most important, inverse predictor of circulating ACBP in multivariate analysis (standardized β=-0.839; p<0.001). Furthermore, AKD increased ACBP concentrations almost 3-fold (p<0.001). Increased ACBP levels were not caused by augmented mACBP mRNA expression in different tissues of CKD mice in vivo or in indoxyl sulfate-treated adipocytes in vitro. CONCLUSIONS: Circulating ACBP inversely associates with renal function, most likely through renal retention of the cytokine. Future studies need to investigate ACBP physiology in malnutrition-related disease states, such as CKD, and to adjust for markers of renal function. Frontiers Media S.A. 2023-05-23 /pmc/articles/PMC10242139/ /pubmed/37288304 http://dx.doi.org/10.3389/fendo.2023.1152444 Text en Copyright © 2023 Schürfeld, Sandner, Hoffmann, Klöting, Baratashvili, Nowicki, Paeschke, Kosacka, Kralisch, Bachmann, Frille, Dietel, Stolzenburg, Blüher, Zhang, Harris, Isermann, Stumvoll, Tönjes and Ebert https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Schürfeld, Robin Sandner, Benjamin Hoffmann, Annett Klöting, Nora Baratashvili, Ekaterine Nowicki, Marcin Paeschke, Sabine Kosacka, Joanna Kralisch, Susan Bachmann, Anette Frille, Armin Dietel, Anja Stolzenburg, Jens-Uwe Blüher, Matthias Zhang, Ming-Zhi Harris, Raymond C. Isermann, Berend Stumvoll, Michael Tönjes, Anke Ebert, Thomas Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor |
title | Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor |
title_full | Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor |
title_fullStr | Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor |
title_full_unstemmed | Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor |
title_short | Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor |
title_sort | renal function is a major predictor of circulating acyl-coa-binding protein/diazepam-binding inhibitor |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10242139/ https://www.ncbi.nlm.nih.gov/pubmed/37288304 http://dx.doi.org/10.3389/fendo.2023.1152444 |
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